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EC number: 406-250-0 | CAS number: 72619-32-0 HALOXYFOP R-(+)-ME HERBICIDAL CHEMICAL
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EEC: Directive 97/69/EEC, of July 31, 1992 Adapting to Technical Progress for the Seventeenth Time Council Directive 67/548/EEC, EEC Publication No. L383A, 1992
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl (R)-2-(4-(3-chloro-5-trifluoromethyl-2-pyridyloxy)phenoxy)propionate
- EC Number:
- 406-250-0
- EC Name:
- Methyl (R)-2-(4-(3-chloro-5-trifluoromethyl-2-pyridyloxy)phenoxy)propionate
- Cas Number:
- 72619-32-0
- Molecular formula:
- C16H13ClF3NO4
- IUPAC Name:
- methyl 2-(4-{[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy}phenoxy)propanoate
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Haloxyfop-R Methyl Ester Technical
Lot #: NB10150101 (TSN101748)
Purity: 98.4%
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Type and composition of metabolic activation system
- Source of S9 : Molecular Toxicology, Inc.
- Method of preparation of S9 mix: The homogenate was prepared from male Sprague-Dawley rats that had been injected (i.p.) with Aroclor™ 1254 (200 mg per ml in com oil) at 500 mg/kg.
- Concentration or volume of S9 mix: 1.00 mL - Test concentrations with justification for top dose:
- Dose range finding study: 6.67, 10.0, 33.3, 66.7, 100, 333, 667, 1000, 3330, 5000 µg/plate
Mutagenicity study: 33.3, 100, 333, 1000, 3330, 5000 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Dimethylsulfoxide (DMSO)
- Justification for choice of solvent/vehicle: At 100 mg per mL, which was the most concentrated stock dilution prepared, the test article formed a transparent, beige solution. The test article remained a solution in all succeeding dilutions prepared for the mutagenicity assay.
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- other: 2-aminoanthracene: TA100, TA1535, TA1537 (2.5 µg; with S9); WP2uvrA (25.0 µg; with S9), ICR-191: TA1537 (2.0 µg; without S9)
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration: Triplicate
- Number of independent experiments: Two (initial mutagenicity assay and confirmatory assay)
METHOD OF TREATMENT/ EXPOSURE:
- Test substance added in medium: Preincubation
TREATMENT AND HARVEST SCHEDULE:
- Preincubation period: 20 ± 2 minutes
- Exposure duration/duration of treatment: 52 ± 4 hour
METHODS FOR MEASUREMENT OF CYTOTOXICITY
- Method: Background growth inhibition - Evaluation criteria:
- For a test substance to be considered positive, it had to produce at least a 3-fold (TA98, TA1535, TA1537, and WP2uvrA) or 2-fold (TA100) dose related and reproducible increase in the mean revertants per plate of at least one tester strain over the mean revertants per plate of the appropriate vehicle control. An observed response which did not meet all three of the above criteria (magnitude, dose-responsiveness, reproducibility) was not evaluated as positive.
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Indications of cytotoxicity were observed with tester strain TA100 with S9 mix at 3330 µg/plate and above as evidenced by a slight reduction of the bacterial background lawn.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: A dose range finding study was conducted on the test article using tester strains TA100 and WP2uvrA in both the presence and absence of S9 mix (one plate per dose). Ten doses of test substance, from 5000 to 6.67 µg per plate, were tested. Indications of cytotoxicity were observed with tester strain TA100 in the presence of S9 mix at 3330 ug per plate and above as evidenced by a slight reduction of the bacterial background lawn. No cytotoxicity was observed with tester strain TA100 in the absence of S9 mix or with WP2uvrA in either the presence or absence of S9 mix as evidenced by a normal background lawn and no decrease in the number of revertants per plate. Slight article precipitate was observed at 3330 µg per plate and above in the presence of S9 mix and at 1000 and 3330 µg per plate in the absence of S9 mix. Moderate test article precipitate was observed at 5000 µg per plate in the absence of S9 mix.
Any other information on results incl. tables
Table-1: Mutagenicity assay results (mean revertants per plate)
Conc. (µg/plate) |
TA1537 |
TA1535 |
TA98 |
TA100 |
WP2uvrA |
|||||
-S9 |
+ S9 |
-S9 |
+ S9 |
-S9 |
+ S9 |
-S9 |
+ S9 |
-S9 |
+ S9 |
|
DMSO |
6 |
12 |
7 |
9 |
9 |
24 |
96 |
93 |
17 |
13 |
33.3 |
3 |
8 |
8 |
10 |
15 |
30 |
79 |
94 |
16 |
14 |
100 |
4 |
9 |
9 |
13 |
9 |
20 |
100 |
98 |
20 |
12 |
333 |
8 |
9 |
12 |
12 |
13 |
26 |
106 |
101 |
14 |
14 |
1000 |
8 |
9 |
11 |
12 |
9 |
20 |
109 |
80 |
14 |
15 |
3330 |
9 |
11 |
8 |
9 |
9 |
16 |
96 |
70 |
13 |
12 |
5000 |
9 |
8 |
7 |
12 |
11 |
20 |
98 |
71 |
9 |
15 |
Positive cotnrol |
1678 |
168 |
588 |
107 |
178 |
500 |
623 |
787 |
451 |
205 |
Table-2: Confirmatory assay results (mean revertants per plate)
Conc. (µg/plate) |
TA1537 |
TA1535 |
TA98 |
TA100 |
WP2uvrA |
|||||
-S9 |
+ S9 |
-S9 |
+ S9 |
-S9 |
+ S9 |
-S9 |
+ S9 |
-S9 |
+ S9 |
|
DMSO |
7 |
7 |
10 |
11 |
14 |
26 |
92 |
96 |
17 |
15 |
33.3 |
8 |
12 |
10 |
10 |
11 |
28 |
87 |
101 |
14 |
16 |
100 |
4 |
10 |
17 |
13 |
16 |
21 |
90 |
96 |
14 |
16 |
333 |
7 |
10 |
11 |
10 |
13 |
25 |
91 |
86 |
17 |
12 |
1000 |
9 |
5 |
10 |
8 |
11 |
22 |
84 |
103 |
15 |
14 |
3330 |
7 |
8 |
10 |
11 |
12 |
19 |
89 |
85 |
14 |
15 |
5000 |
7 |
6 |
15 |
10 |
9 |
26 |
102 |
74 |
18 |
13 |
Positive cotnrol |
1898 |
153 |
710 |
108 |
243 |
423 |
673 |
979 |
547 |
258 |
Applicant's summary and conclusion
- Conclusions:
- Negative in reverse bacterial mutation test
- Executive summary:
The test substance was evaluated in the Salmonella-Escherichia coli/mammalian-microsome reverse mutation assay following OECD guieline 471 and U S EPA OPPTS 870.5100. The tester strains used in this study were Salmonella typhimurium tester strains TA98, TA100, TA1535, TA1537, and Escherichia coli tester strain WP2uvrA. The assay was conducted using 6 doses of test substance in both the presence and absence of an externally supplied metabolic activation system (S9) along with concurrent vehicle and positive controls. The concentration of the test material ranged from 33.3 to 5000 µg/plate in the presence and absence of metabolic activation. The results of the initial mutagenicity assay were confirmed in an independent experiment. The test material did not induce a positive increase in the number of revertants per plate of any of the tester strains either in the presence or absence of microsomal enzymes (S9). Hence the test substance was classified as negative in this bacterial mutagenicity test under the experimental conditions used.
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