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EC number: 942-597-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial photocatalytic activity
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- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September - December, 2014
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- 1986
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- GR-50-1408 is the Givaudan identification code which was employed for ROSYFOLIA during the early, developmental and testing period.
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge :
Activated sludge was obtained from a water treatment plant treating predominantly domestic sewage (Bois-de Bay, 1242 Satigny, Switzerland). The sludge was kept aerobic until being used on the next day. The pH was 8.0.
The suspended solids content was determined by drying 50 ml of the sludge and weighing: dry weight of suspended solids: 8.90 g/l.
By adding 2950 ml of water to 1500 ml sludge, a concentration of suspended solids of 3.0 g/l was obtained. With the final dilution during the test, a concentration (i.e. in the range 0.8 to 1.6 g/l as recommended in ISO 8192-1986) was obtained.
Synthetic sewage feed :
A synthetic sewage feed was made by dissolving the following amounts of substances in 1 litre of tap water:
- 16.0 g peptone (from casein trypsin-digested)
- 11.0 g yeast extract powder
- 3.0 g urea
- 0.7 g NaCl
- 0.4 g CaCl2 · 2 H2O
- 0.3 g MgSO4 · 7 H2O
- 2.8 g K2HPO4 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- The whole experiment was carried out at 20 ± 2°C (temp controlled water bath)
- pH:
- Activated sludge was obtained from a water treatment plant treating predominantly domestic sewage (Bois-de Bay 1242 Satigny, Switzerland). The sludge was kept aerobic until being used on the next day. The pH was 8.0.
- Nominal and measured concentrations:
- The test item was tested as a limit test at the nominal test concentration of 100 mg/L.
- Details on test conditions:
- The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed is measured after a contact time of 3 hours.
The respiration rate of the same activated sludge in the presence of several concentrations of the test substance under otherwise identical conditions is also measured.
The inhibitory effect of the test substance at a particular concentration is expressed as a % of the mean respiration rates of 6 controls. An EC50 value if calculated from determinations performed on a range of test substance conditions.
Note: the highest conc tested is 100mg/L
3,5-dichlorophenol was used as a reference substance to check the activity of the sludge: its EC50 (3hours) should be within the range 2-25mg/L.
A synthetic sewage feed was made by dissolving the following amounts of substances in 1 litre of tap water:
16.0g peptone (from casein trypish-digested)
11.0g yeast exract powder
3.0g urea
0.7g NaCl
0.4g CaCl2 – 2 H2O
0.3g MgSO4 – 7H2O
2.8g K2HPO4
Activated sludge was obtained from a water treatment plant treating predominantly domestic sewage (Bois-de Bay 1242 Satigny, Switzerland). The sludge was kept aerobic until being used on the next day. The pH was 8.0.
The suspended solids content was determined by drying 50ml of the sludge and weighing: dry weight of suspended solids: 8.90g/l.
By adding 2950ml of water to 1500ml sludge, a concentration of suspended solids of 3.0g/l was obtained. With the final dilution during the test, a concentration (i.e. in the range 0.8 to 1.6 g/l as recommended in ISO 8192-1986) was obtained. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: The average % inhibition at the limit test concentration of 100 mg/L was 34.2% (29.5%, 36.6%, 36.6%)
- Details on results:
Conditions for the validity of the test:
- The variation coefficient of the controls was 15.0%. This is within the validity range (30%).
- the EC50 (3 hours) of 3,5-dichlorophenol is between 2 and 25 mg/L.
The tested concentration of the test item (100 mg/l) produced inhibitions below 50% (range 29.5 to 36.6%)
Therefore:
The EC50 (3 hours) of GR-50-1408 is > 100 mg/l- Results with reference substance (positive control):
- The EC50 (3 hours) of 3,5-dichlorophenol is between 2 and 25 mg/l :
- Average Inhibition % at 2 mg/L = 20.1%
- Average Inhibition at 25 mg/L = 56.2% - Validity criteria fulfilled:
- yes
- Conclusions:
- The tested concentration (100 mg/L) produced inibitions below 50% (range 29.5 to 36.6%).
Therefore EC50(3h) of GR-50-1408 > 100 mg/L
Reference
The results of the test are tabulated below.
Conditions for the validity of the test:
The variation coefficient of the controls was 15.0%. This is within the validity range (30%) and the EC50 (3hours) of 3,5-dichlorophenol is between 2 and 25 mg/L.
vessel |
pH |
O2 (mg/l) |
Time (min) |
rate |
% inhibition |
Conc. Final (mg/l) |
Log (conc) |
C1 |
8.5 |
1.54 |
5.0 |
0.31 |
- |
- |
- |
C2 |
8.4 |
1.54 |
5.0 |
0.31 |
- |
- |
- |
C3 |
8.4 |
1.55 |
5.0 |
0.31 |
- |
- |
- |
S1 |
8.1 |
1.25 |
5.0 |
0.25 |
29.5 |
100.0 |
2.00 |
S2 |
8.2 |
1.13 |
5.0 |
0.23 |
36.6 |
100.0 |
2.00 |
S3 |
8.3 |
1.13 |
5.0 |
0.23 |
36.6 |
100.0 |
2.00 |
R1 |
8.4 |
1.38 |
5.0 |
0.28 |
22.5 |
2.0 |
0.30 |
R2 |
8.3 |
1.38 |
5.0 |
0.28 |
22.5 |
2.0 |
0.30 |
R3 |
8.4 |
1.50 |
5.0 |
0.30 |
15.4 |
2.0 |
0.30 |
R4 |
8.4 |
0.88 |
5.0 |
0.18 |
50.7 |
25.0 |
1.40 |
R5 |
8.4 |
0.75 |
5.0 |
0.15 |
57.7 |
25.0 |
1.40 |
R6 |
8.5 |
0.71 |
5.0 |
0.14 |
60.2 |
25.0 |
1.40 |
C4 |
8.4 |
1.88 |
5.0 |
0.38 |
- |
- |
- |
C5 |
8.4 |
2.13 |
5.0 |
0.43 |
- |
- |
- |
C6 |
8.5 |
2.00 |
5.0 |
0.40 |
- |
- |
- |
Description of key information
An activated sludge respiration inhibition test was performed with GR-50-1408 in line with OECD Guideline for the Testing of Chemicals No. 209. In a limit test a test concentration of 100mg/L was tested. The results can be expressed as follows:
The EC50(3h) of GR-50-1408 > 100 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
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