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EC number: 812-549-0 | CAS number: 881539-89-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Feb - 02 Mar 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- yes
- Remarks:
- Temperature during test was 20.1–23.6 °C (OECD 201: 21-24 °C ± 2°C), pre-culture preparation: 5 days before test start (OECD 201: 2-4 days before stest start). As exponential growth was observed in the blank control, this was stated as uncritical.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Rheinland-Pfalz, Mainz, Germany
Test material
- Reference substance name:
- disodium 4-[(1-hydroxyprop-2-en-1-yl)amino]-4'-(prop-2-enamido)-[1,1'-biphenyl]-2,2'-disulfonate
- EC Number:
- 812-549-0
- Cas Number:
- 881539-89-5
- Molecular formula:
- C18H14N2Na2O8S2
- IUPAC Name:
- disodium 4-[(1-hydroxyprop-2-en-1-yl)amino]-4'-(prop-2-enamido)-[1,1'-biphenyl]-2,2'-disulfonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), 10, 22, 46, 100 and 200 mg/L (0, 72 h)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution containing 220.0 mg/L in algal medium, lower treatments were prepared by dilution of this stock solution with algal medium
- Controls: nutrient medium only mixed with algal pre-culture
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- SAG Strain Number: 86.81
- Source (laboratory, culture collection): obtained in January 2016 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen, Germany)
- Age of inoculum (at test initiation): Five days before the start of each test, an aliquot of the permanent culture was brought into nutrient medium and incubated under continuous lighting for 96 hours (5000 lux, 20.4 – 23.1°C)
- Method of cultivation: Kept as stock culture on solid agar at 2 - 8°C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 20.1 - 23.6°C
- pH:
- 7.4 - 7.8
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 10, 22, 46, 100 and 220 mg/L
Measured concentrations initial (t-=0) /test end (t-72h):- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks, total volume 65 mL
- Type: open: covered with perforated plastic foil acting as a stopper
- Fill volume: 45 ± 1 mL
- Aeration: no
- Initial cells density: 2.3*10^3 cells/mL
- Control end cells density: 157.623*10^3
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes: OECD TG 201 medium
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: pH (0, 72 h)
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous light
- Light intensity and quality: 5000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : cell number (0, 24, 48, 72 h), appearance of the algae (72 h)
- Determination of cell concentrations: electronic particle counter (Cell Counter Beckman Coulter Z2)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.09 - 2.2- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): normal and healthy shape in all tested concentration - Results with reference substance (positive control):
- - 72h ErC50: 0.90 mg/L (95% confidence limits: 0.77 – 1.1 mg/L)
- Reference substance results are within the recommended range of 1.19 mg/L ± 0.27 mg/L (ISO guideline 8692) - Reported statistics and error estimates:
- The estimation of the biological data was accomplished using the software ToxRat® Professional, version 3.2.1.
Any other information on results incl. tables
Table 1: Cell number/mL
Nominal Concentration in mg/L |
Parameter |
Cell Number/mL |
|||
0 h |
24 h |
48 h |
72 h |
||
Blank control |
Mean |
2340 |
8747 |
36457 |
157623 |
Blank control |
SD |
0 |
884 |
2310 |
11232 |
10 |
Mean |
2340 |
8587 |
35660 |
155993 |
10 |
SD |
0 |
147 |
1652 |
15427 |
22 |
Mean |
2340 |
8647 |
35913 |
156287 |
22 |
SD |
0 |
162 |
1508 |
18212 |
46 |
Mean |
2340 |
8780 |
34520 |
146667 |
46 |
SD |
0 |
473 |
2589 |
23275 |
100 |
Mean |
2340 |
9000 |
35253 |
145593 |
100 |
SD |
0 |
714 |
1798 |
6972 |
220 |
Mean |
2340 |
9320 |
34700 |
134393 |
220 |
SD |
0 |
278 |
322 |
4302 |
Table 2: Daily growth rates of the blank controls
Growth rates |
days 0 – 1 |
days 1 – 2 |
days 2 – 3 |
CV of sectional daily growth rates |
days 0 – 3 |
Replicate 1 |
1.280 |
1.338 |
1.518 |
9% |
1.379 |
Replicate 2 |
1.340 |
1.427 |
1.532 |
7% |
1.433 |
Replicate 3 |
1.178 |
1.634 |
1.465 |
16% |
1.426 |
Replicate 4 |
1.354 |
1.396 |
1.482 |
5% |
1.411 |
Replicate 5 |
1.472 |
1.297 |
1.387 |
6% |
1.385 |
Replicate 6 |
1.261 |
1.488 |
1.399 |
8% |
1.383 |
Mean |
1.314 |
1.430 |
1.464 |
9% |
1.403 |
Standard deviation |
0.100 |
0.120 |
0.060 |
|
0.024 |
CV |
8% |
8% |
4% |
|
2% |
Table 3: Measured concentration and % of nominal
Nominal Concentration Test Item |
Measured concentration t = 0 h |
Measured concentration t = 72 h |
% of Nominal concentration t = 0 h |
% of Nominal concentration t = 72 h |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
< LOQ |
<LOQ |
-- |
-- |
10 |
8.27 |
9.64 |
82.74 |
96.39 |
22 |
20.85 |
22.35 |
94.77 |
101.58 |
46 |
43.01 |
45.16 |
93.50 |
98.17 |
100 |
96.00 |
98.24 |
96.00 |
98.24 |
220 |
204.48 |
216.84 |
92.95 |
98.56 |
LOQ (Limit of quantification) = 4 mg/L
The recovery of the QC sample of 93.7% (0h) and 96.5% (72h) was taken into account
Table 4: Validity criteria for OECD 201.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
67 |
yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35% |
9% |
yes |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. |
2% |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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