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EC number: 905-459-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
non-sensitizing to the skin in guinea pigs
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.6 (Skin Sensitisation)
- GLP compliance:
- no
- Type of study:
- Buehler test
- Justification for non-LLNA method:
- study performed in 1984, before the validation of the LLNA method
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- not specified
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: acetone/dioxane/guinea pig fat (7:2:1)
- Concentration / amount:
- 10% w/v in FCA
- Route:
- intradermal
- Vehicle:
- other: acetone/dioxane/guinea pig fat (7:2:1)
- Concentration / amount:
- 10% w/v in FCA
- Details on study design:
- The Kodak methods employed both a) topical application of the test material in a unique solvent designed to enhance penetration and dermal absorption and b) subcutaneous injection of the material into the foot pad of the test guinea pigs. The solvent that was used was prepared in the laboratory and consisted of 70% acetone, 20% dioxane, and 10% guinea pig fat. The skin was also depilated prior to application to enhance contact and absorption. To stimulate the immune system, Freund’s Complete Adjuvant was injected into the guinea pig footpad.
- Positive control substance(s):
- not specified
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 10%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 10%. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Remarks on result:
- not measured/tested
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- positive control
- Remarks on result:
- not measured/tested
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Based upon the results of this study, p-diisopropylbenzene is not a dermal sensitizer in guinea pigs
- Executive summary:
In a Kodak standard dermal sensitization assay that is similar to the Buehler method, both topical and subcutaneous injections of test materials suspended or dissolved in agents known to stimulate the immune system. The Kodak method utilized ten guinea pigs that were treated by both a) topical application of the test material in a unique solvent designed to enhance penetration and dermal absorption and b) subcutaneous injection of the material into the foot pad of the test guinea pigs. The solvent that was used was prepared in the laboratory and consisted of 70% acetone, 20% dioxane, and 10% guinea pig fat. The skin was also depilated prior to application to enhance contact and absorption. To stimulate the immune system upon challenge, the test material was incorporated into Freund's Complete Adjuvant and was injected into the footpad of test animals. Following induction, animals were subjected to challenge with the test material that was evaluated 24 hours after exposure, wherein none of the test animals displayed evidence of dermal sensititization.
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- GLP compliance:
- yes
- Type of study:
- other: Kodak method
- Justification for non-LLNA method:
- study performed in 1985, before the validation of the LLNA method
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- male/female
- Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- No. of animals per dose:
- 10
- Details on study design:
- The Buehler test used three 6-hour applications of test substance at one-week intervals, followed by a challenge two weeks later.
- Positive control substance(s):
- no
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 0% v/v
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: 0% v/v. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 100% v/v
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 100% v/v. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Group:
- positive control
- Remarks on result:
- not measured/tested
- Remarks:
- no information available
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test substance, m-diisopropylbenzene, is not sensitizing in guinea pigs
- Executive summary:
The sensitization potential of m-diisopropylbenzene was assessed in guinea pigs using a Buehler test design. Following 3 weekly applications of a 100% test substance, animals were challenged 2 weeks later by application of a 100% test substance. There were no signs of sensitization in any of the test animals. Consequently, the test substance was determined to be non-sensitizing in guinea pigs.
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.6 (Skin Sensitisation)
- GLP compliance:
- yes
- Type of study:
- other: Kodak method
- Justification for non-LLNA method:
- study performed in 1985, before the validation of the LLNA method
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- male/female
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: 70% acetone+20% dioxane+10% guinea pig fat
- Concentration / amount:
- 1% v/v
- Route:
- intradermal
- Vehicle:
- other: 70% acetone+20% dioxane+10% guinea pig fat
- Concentration / amount:
- 10% v/v
- No. of animals per dose:
- 10
- Details on study design:
- The Kodak test involved induction by application of 0.05 mL of a 1% solution of m-diisopropylbenzene in Freund's Complete Adjuvant (FCA). Prior to application, the skin was also depilated prior to application to enhance contact and absorption. Subsequently, animals were challenged by subcutaneous injection of a 10% v/v solution of the material into the foot pad of the test guinea pigs. The solvent that was used was prepared in the laboratory and consisted of 70% acetone, 20% dioxane, and 10% guinea pig fat.
- Challenge controls:
- Guinea pigs that were treated during the induction phase with FCA without test substance
- Positive control substance(s):
- no
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 0% v/v
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: 0% v/v. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 10% v/v
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 10% v/v. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test substance, m-diisopropylbenzene, is not sensitizing in guinea pigs
- Executive summary:
The dermal application of 0.05 mL of a 1% solution of m-diisopropylbenzene in Freund's Complete Adjuvant (FCA) topically to guinea pigs produced slight to moderate erythema and slight edema after 1 -3 days. Animals were subsequently challenged by subcutaneous injection of a 10% v/v solution of the material into the foot pad of the test guinea pigs. At 24 hours, there was no evidence of sensitization in any of the test guinea pigs. Consequently, m-diisopropylbenzene was determined to be non-sensitizing in guinea pigs.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
No study is available with DIPB (reaction mass of 1,3- and 1,4- isomers), but data are available on each isomer.
1,3 -DIPB:
The sensitization potential of m-diisopropylbenzene was assessed in guinea pigs using a Buehler test design. Following 3 weekly applications of a 100% test substance, animals were challenged 2 weeks later by application of a 100% test substance. There were no signs of sensitization in any of the test animals.
In a supporting study, the dermal application of 0.05 mL of a 1% solution of m-diisopropylbenzene in Freund's Complete Adjuvant (FCA) topically to guinea pigs produced slight to moderate erythema and slight edema after 1 -3 days. Animals were subsequently challenged by subcutaneous injection of a 10% v/v solution of the material into the foot pad of the test guinea pigs. At 24 hours, there was no evidence of sensitization in any of the test guinea pigs.
1,4 -DIPB:
In a Kodak standard dermal sensitization assay that is similar to the Buehler method, both topical and subcutaneous injections of test materials suspended or dissolved in agents known to stimulate the immune system. The Kodak method utilized ten guinea pigs that were treated by both a) topical application of the test material in a unique solvent designed to enhance penetration and dermal absorption and b) subcutaneous injection of the material into the foot pad of the test guinea pigs. The solvent that was used was prepared in the laboratory and consisted of 70% acetone, 20% dioxane, and 10% guinea pig fat. The skin was also depilated prior to application to enhance contact and absorption. To stimulate the immune system upon challenge, the test material was incorporated into Freund's Complete Adjuvant and was injected into the footpad of test animals. Following induction, animals were subjected to challenge with the test material that was evaluated 24 hours after exposure, wherein none of the test animals displayed evidence of dermal sensititization.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
Based on the classification criteria of UN/EU GHS, and given the absence of positive reactions in the available skin sensitisation data with 1,3 -DIPB and 1,4 -DIPB, DIPB (as a reaction mass of 1,3- and 1,4 -isomers) is not classified as a skin sensitizer.
No data are available for respiratory sensitisation; therefore no conclusion can be made on the classification of this endpoint.
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