Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-582-3 | CAS number: 1333-22-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental Phase: 20 July 2001 to 03 August 2001. Report issued: 26 November 2001.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken for analysis of total and dissolved copper at the start (0 hours) and at the end (72 hours) of the experiment from both preliminary and definitive tests. Samples were taken from the preparations remaining after filling the test and control flasks at the start and from the pooled replicates at the end of the tests.
Two sets of samples were taken for analysis of copper at each sampling time. One set was unfiltered (for total copper) and one set was filtered (for dissolved copper) using 0.45 μm 'Acrodisc' filters. Each 25 ml sample was acidified with 0.5 ml of 70% nitric acid (AR grade). The samples were stored in a refrigerator prior to transportation to the analytical laboratory at the end of the study for analysis of copper. - Vehicle:
- no
- Details on test solutions:
- For the preliminary test 31.98 mg of the test substance as received (tribasic copper sulphate technical) was weighed out and made up to 1000 ml with algal medium. This stock solution was stirred at high speed using a magnetic stirrer for 19 hours before test dilutions were prepared. Ten minutes of ultrasonication was also applied to disperse the test substance, which consisted of a paste. After dispersion a very pale blue slightly cloudy solution was formed.
Test dilutions were prepared by taking aliquots of the stock solution while it was still being stirred at high speed. The aliquots taken were weighed to the nearest 0.01g before making up to 500 ml with the test medium. - Test organisms (species):
- other: Selenastrum capricornutum
- Details on test organisms:
- The alga was originally obtained from the CCAP, Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria. A culture in liquid medium containing Jaworski's medium was received at CEMAS on 06 June 2001 from CCAP and designated SC05. Sub-cultures were held in liquid medium until used for testing.
The inoculum used for the test was obtained from cultures growing in the medium recommended by CCAP (Jaworski's medium). From experience gained during the culture of Selenastrum capricornutum it was found that variability in growth between replicates was reduced when inoculum was taken from cultures growing in Jaworski's medium, rather than those growing in the medium described in EEC92/69 Part C3, l383, 1992. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- Not stated
- Test temperature:
- During the definitive test temperatures ranged from 21.5°C to 23.5°C with a mean value of 22.6°C.
- pH:
- During the definitive test the pH ranged from 7.50 to 7.70 with a mean value of 7.60.
- Dissolved oxygen:
- Not stated
- Salinity:
- Not stated
- Conductivity:
- Not stated
- Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 26.5 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 318.6 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 30.9 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 92.2 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Reported statistics and error estimates:
- A t-test was undertaken on the growth curves.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, growth of Selenastrum capricornutum was almost completely inhibited at and above 3103.3 μg tribasic copper sulphate technical/L. Growth was significantly (P=0.05) inhibited at 92.2 μg/L, but was not affected at 30.9 μg/L. The LOEC was therefore 92.2 μg tribasic copper sulphate technical/L (equivalent to 29.7 μg Cu/L) and the NOEC was 30.9 μg tribasic copper sulphate technical/L (equivalent to 10.0 μg Cu/L). The EC50 was 318.6 μg/L.
- Executive summary:
Introduction
This study was carried out to determine the toxicity of the tribasic copper sulphate technical to the green algae Selenastrum capricornutum . The test was conducted following the method described in:
OECD test guideline 201, Freshwater Alga and Cyanobacteria, Growth Inhibition Test. Adopted 23 March 2006.
And
EU test guideline No.L383, A/179-186, C3 Algal Inhibition Test, (Official Journal of the European Communities, 29.12.92).
Method
The alga was exposed to a series of concentrations of tribasic copper sulphate technical prepared by vigorously mixing the tribasic copper sulphate technical with the dilution water for approximately 24 hours. A preliminary or range finding test was carried out to establish a range of concentrations for use in the definitive test. For the definitive test the following concentrations of tribasic copper sulphate technical were used; 0 (control), 9.2, 30.9, 92.2, 310.9, 930.6, 3103.3 and 9309.6 μg/L.
The test vessels consisted of 250 ml conical flasks containing 100 ml of the test or control medium. The vessels were continuously illuminated and the pH and temperature of the test solutions in the definitive test ranged from 7.50 to 7.70 and 21.5°C to 23.5°C respectively. Algal growth in each vessel was measured by carrying out absorbance measurements on samples removed each day. Growth was determined in three ways; (1) final cell numbers at 72 hours, (2) rate of increase in cell numbers per day and (3) areas under the growth curves. Samples of filtered (0.45 μm) and unfiltered exposure media for the analytical determination of dissolved and total copper concentrations were taken at the start and end of the preliminary and the definitive tests.
Results and Conclusion
Based on nominal concentrations, growth of Selenastrum capricornutum was almost completely inhibited at and above 3103.3 μg tribasic copper sulphate technical/L. Growth was significantly (P=0.05) inhibited at 92.2 μg/L, but was not affected at 30.9 μg/L. The LOEC was therefore 92.2 μg tribasic copper sulphate technical/L (equivalent to 29.7 μg Cu/L) and the NOEC was 30.9 μg tribasic copper sulphate technical/L (equivalent to 10.0 μg Cu/L). The EC50 was 318.6 μg/L.
Reference
Description of key information
Based on nominal concentrations, growth ofSelenastrum capricornutumwas almost completely inhibited at and above 3103.3 μg tribasic copper sulphate technical/L. Growth was significantly (P=0.05) inhibited at 92.2 μg/L, but was not affected at 30.9 μg/L. The LOEC was therefore 92.2 μg tribasic copper sulphate technical/L (equivalent to 29.7 μg Cu/L) and the NOEC was 30.9 μg tribasic copper sulphate technical/L (equivalent to 10.0 μg Cu/L). The EC50 was 318.6 μg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.319 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.