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EC number: 279-075-9 | CAS number: 79135-28-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From August 29th to September 26th, 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Details on the read-across are available in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Deviations:
- yes
- Remarks:
- sterilization instead of steril filtration; determination of bacteria concentration: by plating; inoculum: effluent of a domestic sewage treatment plant
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (old version) (Ready Biodegradability: Modified AFNOR Test)
- Principles of method if other than guideline:
- SOP MBA 001 02, with modification of inoculum: effluent of a domestic sewage treatment plant
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Mixture of polyvalent bacteria collected on 29.08.89, effluent of a domestic sewage treatment plant (ARA-Rhein).
Bacteria concentration:5 × 10^5/ml - Duration of test (contact time):
- 28 d
- Details on study design:
- Test concentration: 40 mg/l DOC nominal
Water/ nutrient: according to the specification of the EEC Annex V, Part. C.4 resp. OECD method 301A.
Temperature: 23 ± 1°C
Duration: 28 days
Lighting: indirect daylight
Estimation technique: TOC/DOC
SHIMADZU TOC-500 - Reference substance:
- other: glucose
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: DOC removal was 0 in the three replicates
- Results with reference substance:
- Biodegradation corrected by the blank control and measured as DOC (mg/i) is: 100 % (28th day)
- Validity criteria fulfilled:
- no
- Remarks:
- pH and detailed concentration of the medium are missing
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The substance is not readily biodegradable. No inhibition on the activity of the bacteria can be found (degradation of the reference substance > 95 %)
- Executive summary:
The determination of biodegradation in percent of DOC (Dissolved Organic Carbon) was evaluated on the test substance. Modifications to the guideline were applied: sterilization instead of steril filtration; determination of bacteria concentration: by plating; type of inoculum: effluent of a domestic sewage treatment plant.
Test substance was found to be not readily biodegradable under the conditions of the test. Biodegradation corrected by blank control and measured as DOC (mg/l) was 0 % (on day 28).
Reference
The measured was done in triplicate.
0 %DOC Removal (corrected by the blank control) |
% DOC removal after . . . days |
||||
Flask |
3 |
7 |
14 |
28 |
|
1 |
0 |
0 |
0 |
0 |
|
2 |
0 |
0 |
0 |
0 |
|
3 |
0 |
0 |
0 |
0 |
|
13 |
89.0 |
91.6 |
85.3 |
100 |
|
14 |
47.5 |
47.6 |
43.6 |
40.8 |
Flask 1,2,3: test material and inoculum
Flask 13: test material, glucose and inoculum (glucose control)
Flask 14: test material, glucose and inoculum (inhibitory action)
Description of key information
Not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
No experimental study is available to assess biodegradation in water of Acid Brown 303. Available data on a structural analogue, i.e. Similar Substance 01, was used in a read-across approach. Details on the read-across are available in section 13.
The biodegradability of Similar Substance 01 was assessed according to EU method C.4 -A (Dissolved Organic Carbon (DOC) Die-Away Test), for 28 days.
The tested substance was found to be not readily biodegradable, based on a DOC removal of 0 % after 28 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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