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Diss Factsheets
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EC number: 701-300-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 November 2011 - 01 december 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for possible analysis were taken from all test concentrations and the control:
Frequency: at t=0 h, t=24 h and t=72 h
Volume: 4.8 ml
Storage: Samples were stored in a freezer until analysis.
Compliance with the Quality criteria regarding maintenance of actual concentrations was demonstrated by running a test vessel at the highest substance concentration but without algae and samples for analysis were taken at the start, after 24 hours of exposure and at the end of the test period.
Additionally, reserve samples of 4.8 ml were taken from all test solutions for possible analysis. - Vehicle:
- no
- Details on test solutions:
- Preparation of test solutions started with aquatic mixtures prepared individually at loading rates of 1.0, 10 and 100 mg/l. The mixtures were treated with ultrasonic waves for 8 minutes and then magnetically stirred for two days. The resulting dispersions were filtered through a 0.45 μm membrane filter (Whatman; RC55) to remove the remaining undissolved material. The resulting filtrates were all clear and colourless.
After preparation, volumes of 50 ml were added to each replicate of the respective test concentration. Subsequently, 1 ml of an algal suspension was added to each replicate providing a cell density of 10^4 cells/ml. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: NIVA CHL 1
- Source (laboratory, culture collection): In-house laboratory culture
- Method of cultivation: Algae stock cultures were started by inoculating growth medium with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light in a climate room at a temperature of 21-24°C. 3 days before the start of the test, cells from the algal stock culture were inoculated in culture medium at a cell density of 1 x 10^4 cells/ml. The pre-culture was maintained under the same conditions as used in the test. The cell density was measured immediately before use.
ACCLIMATION
- Culturing media and conditions (same as test or not): stock culture medium = M1; Pre-culture medium = M2 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
- Hardness:
- 24 mg CaCO3/l
- Test temperature:
- 21.2 - 22.9 ºC
- pH:
- 7.9 - 8.0
- Dissolved oxygen:
- No information
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- No detectable concentrations of either Al, Ce and Tb were present during the 72-hour exposure period. To demonstrate proper preparation of the test solutions an aliquot of the filter residue was analysed. The elements Al, Ce and Tb were observed in the filter residue solution. It demonstrated that the test substance was used for the preparation of the test solutions.
Similar results were obtained for the highest concentration incubated without algae. - Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 ml, all-glass, containing 50 ml of test solution
- Initial cells density: 1 x 10^4 cells/ml
- Control end cells density: 134.4 x 10^4 cells/ml
- No. of vessels per concentration (replicates): 6 for highest concentration, 3 for each lower concentration
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M2 medium
OTHER TEST CONDITIONS
- Photoperiod: Continuously using TLD-lamps of the type „Cool-white‟ of 30 Watt, with a light intensity within the range of 77 to 88 µE.m-2.s-1.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber at beginning of test, spectrophotometer used thereafter.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Combined Limit- Range finding study
- Test concentrations: Filtered solutions prepared at loading rates of 1.0, 10 and 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Remarks on result:
- other: The 72h-EC50 exceeds and the 72h-NOEC equals the maximum soluble concentration of test substance in test medium.
- Details on results:
- The EC50 exceeds and the NOEC equals the maximum soluble concentration of test substance in test medium.
No significant differences were recorded between the values for growth rate at any of the test concentrations when compared to the control group.
Microscopic observations at the end of the test revealed a normal and healthy appearance of the exposed cells when compared to the control. - Results with reference substance (positive control):
- Potassium dichromate reduced growth rate of this fresh water algae species at nominal concentrations of 0.56 mg/l and higher.
The EC50 for growth rate reduction (ERC50: 0-72h) was 1.5 mg/l with a 95% confidence interval ranging from 1.1 to 2.1 mg/l. The historical ranges for growth rate reduction lie between 0.82 and 2.3 mg/l.
Hence, the ERC50: 0-72h for the algal culture tested corresponds with this range. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study with Pseudokirchneriella subcapitata, no reduction of growth rate was recorded in a filtered solution prepared at a loading rate of 100 mg/l after 72 hours of exposure. The EC50 for growth rate reduction exceeded the concentration present in a filtered solution prepared at a loading rate of 100 mg/l, which was considered the maximum soluble concentration in test medium. Due to the very low solubility of CAT in test medium, concentration levels that could affect algal growth could not be reached.
Reference
Description of key information
CAT was tested in a fresh water algal growth inhibition test (OECD 201). No reduction of growth rate was recorded at a 0.45 μm filtered solution prepared at a loading rate of 100 mg/l after 72 hours of exposure.
Key value for chemical safety assessment
Additional information
CAT was tested in a fresh water algal growth inhibition test (OECD 201). Under the conditions of the study with Pseudokirchneriella subcapitata, no reduction of growth rate was recorded at a 0.45 μm filtered solution prepared at a loading rate of 100 mg/l after 72 hours of exposure. The 72h-EC50 for growth rate reduction exceeded the concentration present in a 0.45 μm filtered solution prepared at a loading rate of 100 mg/l, which was considered the maximum soluble concentration in test medium. The 72h-NOEC equaled the maximum soluble concentration in test medium.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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