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EC number: 948-264-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 23, 2018 - December 21, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- July 17, 1992; §14 corrected July 26, 1993
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- dated 22 November 2017
Test material
- Reference substance name:
- Complexation products of bismuth(3+) neodecanoate with propane-1,2-diol, propoxylated and 2,2',2'',2'''-ethylenedinitrilotetraethanol
- EC Number:
- 948-264-4
- IUPAC Name:
- Complexation products of bismuth(3+) neodecanoate with propane-1,2-diol, propoxylated and 2,2',2'',2'''-ethylenedinitrilotetraethanol
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Laboratory preparation:
A filtration through a fine sieve (about 1 mm) was performed in order to remove coarse particles. After removal of any coarse particles, the sludge was washed by decantation in a mineral medium until sludge was considered free from excess substrate or inhibitor.
Inoculum pre-conditioning:
Pre-conditioning consisted in aerating sludge (in mineral medium) for 5-7 days before the test and at the test temperature. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST ITEM PREPARATION
The test item “BiCAT 8842” was tested at 100 mg/L in mineral medium.
The test item was directly introduced with sludge into each test vessels.
The test medium was the mineral medium, a weakly saline aqueous medium
PROTOCOL
Principle:
A measured volume of inoculated medium containing a known concentration of the test item (100 mg/L) as the nominal sole source of Carbon, was stirred in a closed vessel at a constant temperature up to 28 days.
Microbial respiration consumes O2 and releases CO2 which was absorbed by soda lime pellets present in the headspace of flasks. This produces a decrease in pressure in the flask proportional to the amount of oxygen taken up by the microbial population during biodegradation, which was expressed as a percentage of ThOD (Theoretical Oxygen Demand).
Allowance was made for the endogenous activity of the inoculum by running parallel blanks with inoculum but without the test item.
Furthermore, in order to check the procedure, a reference item control (sodium benzoate 60.2 mg/L + inoculum) and a toxicity control (sodium benzoate + inoculum + test item) were run in parallel.
The pH of the contents was measured at the start and the end of the experiment.
Tes system: The inoculum was a fresh sample of activated sludge and was collected from the aeration tank of a sewage treatment plant receiving predominantly domestic sewage.
Incubation:
The test was performed in darkness between 20 to 24°C (constant temperature ±1°C).
MATERIAL AND METHOD
Inoculum preparation:
Sample site = Water treatment plant 'STEP de Lille', 33290 Blanquefort (France) - Tank 1
Date of sampling = May 31, 3018
Inoculum physico-chemical characteristic at the beginning of the test:
Suspended solid = 2.75 g/L
pH = 6.0
Dissolved oxygen = 9.1 mg/L
Activated sludge preparation:
1. Filtration through a 1 mm sieve
2. 3 successive decantations into mineral medium
3. Activated sludge aeration during 5 days
Preparation of the test solutions:
The test item “BiCAT 8842” was tested at 100 mg/L in mineral medium.
The test item was directly introduced with sludge into each test vessels.
The test medium was the mineral medium, a weakly saline aqueous medium.
Reference solution:
Solution of Sodium Benzoate at 1 g/L: weighing of 500.02 mg in 500mL mineral medium.
A solution of sodium benzoate was produced: weighing of 200.15 mg in 200 mL (final volume) mineral medium.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- The theorical Oxygen Demand ThOD(NH4) is 1.922 mg O2 per mg of test item.
The theorical Oxygen Demand ThOD(NO3) is 2.197 mg O2 per mg of test item. - Test performance:
- All validity criteria were successful.
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 9.04
- Sampling time:
- 28 d
- Remarks on result:
- other: See 'Remarks'
- Remarks:
- Evaluation of biodegradation based as ThOD(NH4).
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7.9
- Sampling time:
- 28 d
- Remarks on result:
- other: See remarks
- Remarks:
- Evaluation of biodegradation based as ThOD(NO3).
- Details on results:
- The test item “BiCAT 8842” contains nitrogen according to the elementary analysis, the evaluation of biodegradation has to be based in the following expressed as ThODNH4 and ThODNO3.
Based on ThODNH4, the 10-Day window failed and the degradation rate of the test item “BiCAT 8842” reached 9.04% after 28 days of incubation.
Based on ThODNO3, the 10-Day window failed and the degradation rate of the test item “BiCAT 8842” reached 7.90% after28 days of incubation.
According to the OECD guideline 301F, the test item is not considered to be biodegradable whatever the ThOD.
Biodegradation of the reference item: “Sodium benzoate”:
The reference item “Sodium benzoate” was degraded up to 93.02% after 14 days.
Therefore, it confirms the suitability of the used aerobic sludge inoculum.
Biiodegradation of the Toxicity Control:
A biodegradation of 30.51 and 31.20% after 14 days of incubation was noted in the Toxicity control (containing both of the test item and the reference item) based on ThODNH4. At the end on the test, 28 days of incubation, the result was 37.83% and 41.25% respectively for both replicates.
A biodegradation of 27.89 and 28.52% after 14 days of incubation was noted in the Toxicity control (containing both of the test item and the reference item) based on ThODNO3. At the end on the test, 28 days of incubation, the result was 34.58 and 37.71% respectively for both replicates.
Therefore, the test item was not inhibitory at the tested concentration on the aerobic activated sludge micro-organism whatever the nitrification because degradation was higher than 25% within 14 days (validity criterion).
BOD5 / COD results
- Results with reference substance:
- The reference item “Sodium benzoate” was degraded up to 93.02% after 14 days.
Therefore, it confirms the suitability of the used aerobic sludge inoculum.
Any other information on results incl. tables
Percentage (%) Biodegradation during 28 days with ThOD(NH4) = 1.922 mg O2/mg test item
Time (days) | Toxicity control | Test item | ||||
Measuring system | 25 | 17 | 35 | 24 | 7 | Average |
0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
1 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2 | 20.72 | 20.72 | 0.00 | 0.00 | 0.00 | 0.00 |
3 | 23.12 | 23.60 | 0.00 | 0.00 | 0.00 | 0.00 |
4 | 26.66 | 26.66 | 0.00 | 0.00 | 0.00 | 0.00 |
5 | 28.10 | 27.62 | 0.00 | 0.00 | 0.00 | 0.00 |
6 | 28.61 | 28.13 | 0.00 | 0.00 | 0.00 | 0.00 |
7 | 29.09 | 29.09 | 0.00 | 0.00 | 0.00 | 0.00 |
8 | 29.57 | 29.57 | 0.00 | 0.00 | 0.00 | 0.00 |
9 | 30.02 | 29.55 | 0.00 | 0.00 | 0.00 | 0.00 |
10 | 29.69 | 29.69 | 0.00 | 0.00 | 0.00 | 0.00 |
11 | 29.69 | 30.17 | 0.00 | 0.00 | 0.00 | 0.00 |
12 | 30.17 | 30.17 | 0.00 | 0.00 | 0.00 | 0.00 |
13 | 30.33 | 30.67 | 0.00 | 2.19 | 0.00 | 0.73 |
14 | 30.51 | 31.20 | 0.00 | 4.13 | 0.49 | 1.54 |
15 | 30.51 | 31.54 | 0.00 | 4.13 | 3.40 | 2.51 |
16 | 31.90 | 31.22 | 0.00 | 4.37 | 4.37 | 2.91 |
17 | 33.11 | 33.11 | 0.00 | 4.86 | 4.86 | 3.24 |
18 | 35.16 | 35.51 | 1.94 | 5.64 | 5.64 | 4.41 |
19 | 35.51 | 38.24 | 4.86 | 6.36 | 5.64 | 5.62 |
20 | 36.24 | 38.63 | 4.67 | 6.12 | 6.12 | 5.64 |
21 | 36.08 | 39.50 | 5.88 | 7.34 | 5.88 | 6.36 |
22 | 36.24 | 40.00 | 6.12 | 8.31 | 6.12 | 6.85 |
23 | 37.08 | 39.82 | 6.36 | 8.55 | 7.09 | 7.34 |
24 | 36.96 | 40.70 | 7.09 | 9.28 | 7.09 | 7.82 |
25 | 37.28 | 41.39 | 7.09 | 9.28 | 8.55 | 8.31 |
26 | 37.28 | 41.39 | 7.09 | 10.01 | 8.55 | 8.55 |
27 | 38.15 | 41.23 | 6.85 | 9.76 | 9.76 | 8.79 |
28 | 37.83 | 41.25 | 7.09 | 10.01 | 10.01 | 9.04 |
Percentage (%) Biodegradation during 28 days with ThOD(NO3) = 2.197 mg O2/mg test item
Time (days) | Toxicity control | Test item | ||||
Measuring system | 25 | 17 | 35 | 24 | 7 | Average |
0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
1 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
2 | 18.94 | 18.94 | 0.00 | 0.00 | 0.00 | 0.00 |
3 | 21.13 | 21.57 | 0.00 | 0.00 | 0.00 | 0.00 |
4 | 24.37 | 24.37 | 0.00 | 0.00 | 0.00 | 0.00 |
5 | 25.68 | 25.24 | 0.00 | 0.00 | 0.00 | 0.00 |
6 | 26.15 | 25.71 | 0.00 | 0.00 | 0.00 | 0.00 |
7 | 26.59 | 26.59 | 0.00 | 0.00 | 0.00 | 0.00 |
8 | 27.03 | 27.03 | 0.00 | 0.00 | 0.00 | 0.00 |
9 | 27.44 | 27.01 |
0.00 |
0.00 |
0.00 |
0.00 |
10 |
27.14 |
27.14 |
0.00 |
0.00 |
0.00 |
0.00 |
11 |
27.14 |
27.58 |
0.00 |
0.00 |
0.00 |
0.00 |
12 |
27.58 |
27.58 |
0.00 |
0.00 |
0.00 |
0.00 |
13 |
27.72 |
28.04 |
0.00 |
1.91 |
0.00 |
0.64 |
14 |
27.89 |
28.52 |
0.00 |
3.61 |
0.42 |
1.35 |
15 |
27.89 |
28.83 |
0.00 |
3.61 |
2.97 |
2.19 |
16 |
29.16 |
28.54 |
0.00 |
3.82 |
3.82 |
2.55 |
17 |
30.27 |
30.27 |
0.00 |
4.25 |
4.25 |
2.83 |
18 |
32.14 |
32.45 |
1.70 |
4.93 |
4.93 |
3.85 |
19 |
32.45 |
34.96 |
4.25 |
5.57 |
4.93 |
4.92 |
20 |
33.12 |
35.31 |
4.08 |
5.36 |
5.36 |
4.93 |
21 |
32.98 |
36.10 |
5.14 |
6.42 |
5.14 |
5.57 |
22 |
33.12 |
36.56 |
5.36 |
7.27 |
5.36 |
5.99 |
23 |
33.89 |
36.39 |
5.57 |
7.48 |
6.21 |
6.42 |
24 |
33.77 |
37.21 |
6.21 |
8.12 |
6.21 |
6.84 |
25 |
34.08 |
37.83 |
6.21 |
8.12 |
7.48 |
7.27 |
26 |
34.08 |
37.83 |
6.21 |
8.75 |
7.48 |
7.48 |
27 |
34.87 |
37.68 |
5.99 |
8.54 |
8.54 |
7.69 |
28 |
34.58 |
37.71 |
6.21 |
8.75 |
8.75 |
7.90 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under conditions of OECD Guideline 301F, the test item is not considered to be biodegradable.
- Executive summary:
The aim of this study was to determine the effects of the test item according to the OECD 301F guideline, ready biodegradability within an experimental period of 28 days by respirometric method.
The amount of oxygen taken up by the microbial population during the biodegradation of the test item (corrected by blank
inoculum, run in parallel) was determined from the change in pressure in apparatus and will be expressed as percentage of theoretical Oxygen Demand (ThOD).
All the validity criteria were successful.
Based on ThODNH4, the 10-Day window failed and the degradation rate of the test item “BiCAT 8842” reached 9.04% after 28 days of incubation.
Based on ThODNO3, the 10-Day window failed and the degradation rate of the test item reached 7.90% after
28 days of incubation.
The results obtained show that the test item is not considered to be readily biodegradable whatever the ThOD, after 28 days of incubation.
Under conditions of OECD Guideline 301F, the test item is not considered to be biodegradable.
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