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EC number: 615-984-2 | CAS number: 73547-70-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 August and 2 September 2004.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- yes
- Remarks:
- Deviation was not considered to affect the integrity of the study or the validity of the conclusions drawn
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- The test substance was received at Brixham Environmental Laboratory on 19 May 2004 and assigned the Brixham test substance number 04-0169. The test substance (Batch Sample Ref SP04027/GLP) was supplied as a white/cream solid.
The sample was stored, at ambient temperature, in the container in which it was received until required for testing, when appropriate subsamples were provided for the test operators. - Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Four treatments were prepared. Three of the treatments served as controls; the biological control (containing inoculum only), the procedural control (containing 300 mg C L-1 aniline, as the reference substance, plus inoculum) and a chemical control (containing 10 mg r1 ceftazidime dihydrochloride with no inoculum). The remaining treatment, termed the biotic exposure, contained 10 mg r1 ceftazidime dihydrochloride plus inoculum . After being made up to volume (3000 ml}, the pH of each treatment was measured and adjusted to between 6.5 and 8.0 using 2 M sodium hydroxide or 2 M hydrochloric acid as appropriate. Each treatment was then divided equally between two replicate flasks, providing an initial test volume of 1500 ml. The flasks were covered with aluminium foil to exclude light, provided with gentle aeration and stirred (magnetic stirrer) for 14 days at a nominal 20 to 25°C. Prior to sampling, the volume in each flask was measured and evaporative losses were corrected as necessary by the addition of deionised water. The pH of each flask was recorded on weekdays during the experimental period and adjusted to between 6.5 and 8.0 as necessary.
- Duration of test (contact time):
- ca. 14 d
- Initial conc.:
- ca. 10 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- TOC removal
- Details on study design:
- Principle of the test method
The concentration of ceftazidime dihy
lnoculum
Activated sludge was obtained from Buckland Sewage Treatment Works Newton Abbot, Devon, UK. This works treats sewage of predominantly domestic origin. At the laboratory the activated sludge was kept aerated at room temperature and the pH maintained at approximately 7. The day before the test the activated sludge was allowed to settle, the supernatant liquid decanted and the remaining sludge washed with test nutrient solution. Finally the supernatant was decanted and the total solids concentration determined on the remaining sludge. The activated sludge used in the test was not adapted to the test or reference substances.
Nutrient solution
The test medium was made up according to the OECD 302 B guideline and contained approximately the following nutrients per litre of deionised water: 85 mg of KH2P04, 217.5 mg of K2HP04, 334 mg of Na2HP04.2H20, 5 mg of NH4Cl, 22.5 mg of MgS04.7H20, 36.4 mg of CaCl2.2H20, 0.25 mg of FeCl3.6H20 and 0.40 mg of EDTA (disodium salt).
Test and reference substance solutions
A 100 mg L-1 stock solution of ccftazidirne dihydrochloride was prepared by dissolving a known quantity in deionised water and stirred for 15 minutes.
A 3000 mg C L-1 (ie expressed as mg of carbon per litre) stock solution of aniline was prepared by dissolving a known quantity in deionised water .
Both stock solutions were observed to be clear and colourless.
Experimental design
Four treatments were prepared as shown in Table 1. Three of the treatments served as controls; the biological control (containing inoculum only), the procedural control (containing 300 mg C L-1 aniline, as the reference substance, plus inoculum) and a chemical control (containing 10 mg L-1 ceftazidime dihydrochloride with no inoculum). The remaining treatment, termed the biotic exposure, contained 10 mg L-1 ceftazidime dihydrochloride plus inoculum . After being made up to volume (3000 ml}, the pH of each treatment was measured and adjusted to between 6.5 and 8.0 using 2 M sodium hydroxide or 2 M hydrochloric acid as appropriate. Each treatment was then divided equally between two replicate flasks, providing an initial test volume of 1500 ml. The flasks were covered with aluminium foil to exclude light, provided with gentle aeration and stirred (magnetic stirrer) for 14 days at a nominal 20 to 25°C. Prior to sampling, the volume in each flask was measured and evaporative losses were corrected as necessary by the addition of deionised water.
The pH of each flask was recorded on weekdays during the experimental period and adjusted to between 6.5 and 8.0 as necessary.
Total organic carbon (TOC) analysis
During the course of the test, measured aliquots were removed from the biological and procedural controls for TOC analysis (Ref 2). Prior to analysis the samples were centrifuged, at approximately 13 800 G for 15 minutes, to remove particulate matter. This was performed 0, 1, 3 and 8 hours after the start of the test and on days 1, 4, 7, 12 and 14 .
Specific analysis by HPLC
During the course of the test measured aliquots were removed from the biological and chemical controls and the biotic exposure for specific ceftazidime dihydrochloride analysis. Prior to analysis the samples were centrifuged, at approximately 13 800 G for 15 minutes, to remove the particulate matter. These were taken 0, 1, 3 and 8 hours after the start of the test and on days 1, 4, 7, 12 and 14 for the biotic exposure. The biological and chemical controls were sampled at O hours, plus days 1 and 14. The supernatant was analysed using the high performance liquid chromatography. - Reference substance:
- aniline
- Remarks:
- Aniline (300 mg C L-1)
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- ca. 0
- Sampling time:
- 0 h
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- < 5
- Sampling time:
- 8 h
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- ca. 8
- Sampling time:
- 1 d
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- ca. 29
- Sampling time:
- 4 d
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- ca. 46
- Sampling time:
- 7 d
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- ca. 61
- Sampling time:
- 12 d
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- ca. 65
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- ca. 0
- Sampling time:
- 1 h
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- ca. 0
- Sampling time:
- 3 h
- Results with reference substance:
- The results for the procedural control show that a minimum of 70% aniline biodegradation had been achieved within 14 days, confirming the viability of the activated sludge
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Analysis of the biotic exposure at the end of the 14 day test period showed mean degradation of 65%. Analysis of the chemical control at the end of the 14 day test period showed mean degradation of 31 %. It is, therefore, considered that the degradation of ceftazidime dihydrochloride in this study was partially an abiotic process.
- Executive summary:
Subject: Determination of inhere~t biodegradability (Zahn-Wellens test)
Guideline: OECD Guidelines for the Testing of Chemicals (1993). Test Guideline 302 B, Zahn-Wellens/EMPA Test. Adopted 17 July 1992
Test substance concentration: 10mg L-1
Duration of test: 14 days
Reference substance: Aniline (300 mg C L-1)
Time Ceftazidime dihydrochloride Chemical control Reference substance
0 h - - -
1 h - - -
3 h - - -
8 h <5 - <5
1 d 8 <5 <5
4 d 29 - 34
7 d 46 - 93
12 d 61 - 94
14 d 65 31 98
Comments: Analysis of the biotic exposure at the end of the 14 day test period showed mean degradation of 65%. Analysis of the chemical control at the end of the 14 day test period showed mean degradation of 31 %. It is, therefore, considered that the degradation of ceftazidime dihydrochloride in this study was partially an abiotic process.
Reference
Description of key information
Analysis of the biotic exposure at the end of the 14 day test period showed mean degradation of 65%. Analysis of the chemical control at the end of the 14 day test period showed mean degradation of 31 %. It is, therefore, considered that the degradation of ceftazidime dihydrochloride in this study was partially an abiotic process.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
- Type of water:
- freshwater
Additional information
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