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EC number: 271-272-8 | CAS number: 68527-63-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 September 2021 - 22 December 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted on 1 June 2019
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- lot number of test material: 10419
- Purity, including information on contaminants, isomers, etc.: 93%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient (21 to 29˚C), dry and dark
- Date of Manufacture : 2021/4/23
- Date of Expiry : 2023/4/2 - Analytical monitoring:
- yes
- Remarks:
- - during main study, samples from test item stock solution was collected at 0 hour and at 96 hours; - analytical method: HPLC - test concentrations during main study were prepared by diluting the stock solution
- Details on sampling:
- - Concentrations:
- DRF: 0.25 mg/L, 1.10 mg/L, 4.84 mg/L, 21.30 mg/L and 93.70 mg/L
- main study: 0.129 mg/L, 0.270 mg/L, 0.567 mg/L, 1.190 mg/L and 2.5 mg/L
- Sampling method: collection at 0 hour (fresh solution) and at 96 hours (spent solution). A volume of 9 mL of test samples was collected in duplicates from stock solution. - Vehicle:
- yes
- Remarks:
- tap water
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: common carp
- Source: in-housed maintained procured from Fisheries Research and Information Center (Inland), Hesaraghatta, Bangalore, Karnataka, India.
- Age at study initiation: juvenile
- Length at study initiation: 2.5 to 3.5 cm (dose range finding study) and 3.1 to 3.8 cm (main study)
ACCLIMATION
- Acclimation period: 9 days (48 hours settling-in + 7 days acclimatization) prior to test initiation
- Acclimation conditions: Water temperature during acclimatization was between 22.0 °C to 22.9 °C during dose range finding study and 22.2 °C to 22.9 °C during main study. CaCO3/L and 177.6 mg CaCO3/L and dissolved oxygen content of ˃82.3 and ˃83.9% was maintained during dose range finding study and main study respectively.
- Type and amount of food during acclimation: ad libitum with commercial feed pellets (Kijaro Grow)
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): No mortality was observed during acclimatization of both dose range finding study and main study. - Test type:
- static
- Water media type:
- other: Reverse osmosis purified water (Tap water)
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 176.7 mg/L CaCO3
- Test temperature:
- maintained between 22.1 °C to 22.9 °C
- pH:
- 6.79 to 6.99
- Dissolved oxygen:
- 86.9 to 95.4 %
- Nominal and measured concentrations:
Nominal Conc. (mg/mL) Obtained Conc. (mg/mL) Recovery (%)
Date of Analysis: 29/10/2021
Sample 1 0.00 BLOQ -
Sample 2 10.00 8.97 89.66
Date of Analysis: 02/11/2021
Sample 1 0.00 BLOQ -
Sample 2 10.00 9.81 98.07- Details on test conditions:
- TEST SYSTEM
- Test vessel: thoroughly cleaned rectangular glass aquaria having the water holding capacity of 50 liters. Test vessels were labeled for identification (study no., test item code, study code, group no. and concentration, exposure start and exposure end) prior to experiment initiation.
- No. of organisms per vessel: 7
- Biomass loading rate: 0.27 g and 0.28 g wet weight of fish/L during dose range finding study and main study respectively
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: inhouse
- Total organic carbon: ≤ 2 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12 hours darkness
- Light intensity: 574 to 686 lux (during dose range finding study), 584 to 722 lux (during main study)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 4.4 for dose range finding study; 2.1 for main study
- Range finding study: 0.25 mg/L, 1.10 mg/L, 4.84 mg/L, 21.30 mg/L and 93.70 mg/L
- Test concentrations: 1.10 mg/L, 4.84 mg/L, 21.30 mg/L and 93.70 mg/L
- Results used to determine the conditions for the definitive study: during dose range finding study, no mortalities were recorded in control group and at the tested concentration of 0.25 mg/L. Mortalities of 85.7 %, 100.0 %, 100.0 % and 100 % were observed at the tested concentrations of 1.10 mg/L, 4.84 mg/L, 21.30 mg/L and 93.70 mg/L, respectively - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.497 mg/L
- 95% CI:
- 0.344 - 0.72
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other abnormalities:
Dose Range Finding Study: Clinical signs of hypoactivity, abnormal surface distribution/behavior and abnormal bottom istribution/behavior were observed during the 5 hours observation period at the tested concentration of 4.84 mg/L.
Clinical signs of abnormal surface distribution/behavior and corkscrew swimming were observed during the 2 hours observation period at the tested concentration of 21.30 mg/L.
Clinical signs of surface distribution/behavior were observed during 2 hours observation period at the tested concentrations of 93.70 mg/L.
Main study:
Clinical signs of abnormal bottom distribution/behavior were observed at the tested concentration of 0.270 mg/L during 96 hours of exposure period.
Clinical signs of hypoactivity and abnormal bottom distribution/behavior were observed at the tested concentrations of 0.567 mg/L and 1.190 mg/L during the 96 hours exposure period.
Clinical signs of abnormal bottom distribution/behavior and abnormal surface distribution/behavior were observed at the tested concentration of 2.5 mg/L during the first 30 hours of the exposure period.
- Mortality of control: No mortality was observed at control group - Reported statistics and error estimates:
- The acute Median Lethal Concentration (LC50) of Diethyl sulphate, compound with 2-
(heptadec-8-enyl)-4,5-dihydro-1H-imidazole-1-ethanol (1:1) was evaluated by using the Probit analysis (Finney, 1971) with 95% confidence limits based on the nature of the mortality data. The LC50 values were evaluated with fiducial limits and graph was plotted with percent mortality against test concentration for recommended exposure periods of 96 hours. - Sublethal observations / clinical signs:
During the main study, no clinical signs of toxicity were observed at the control group and at the tested concentration of 0.129 mg/L during the 96 hours of observation period.
Clinical signs of abnormal bottom distribution/behavior were observed at the tested concentration of 0.270 mg/L during 96 hours of exposure period.
Clinical signs of hypoactivity and abnormal bottom distribution/behavior were observed at the tested concentrations of 0.567 mg/L and 1.190 mg/L during the 96 hours exposure period.
Clinical signs of abnormal bottom distribution/behavior and abnormal surface distribution/behavior were observed at the tested concentration of 2.5 mg/L during the first 30 hours of the exposure period.
No mortality was observed in control group and at the tested concentration of 0.129 mg/L during the 96 hours exposure period. Percent mortalities of 14.3 %, 71.4 %, 85.7 % and 100.0 % were observed at the tested concentrations of 0.270 mg/L, 0.567 mg/L, 1.190 mg/L and 2.5 mg/L of test item respectively during the 96 hours exposure period.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hours acute Median Lethal Concentration (LC50) value of Diethyl sulphate, compound with 2-(heptadec-8-enyl)-4,5-dihydro-1H-imidazole-1-ethanol (1:1) is 0.497 mg/L.
- Executive summary:
In accordance to OECD 203, the acute toxicity of Diethyl sulphate, compound with 2-(heptadec-8-enyl)-4,5-dihydro-1H-imidazole-1-ethanol (1:1) has been assessed on fish (Cyprinus carpio (Common carp)).
Percent mortalities of 14.3 %, 71.4 %, 85.7 % and 100.0 % were observed at the tested concentrations of 0.270 mg/L, 0.567 mg/L, 1.190 mg/L and 2.5 mg/L. The 96 hours acute Median Lethal Concentration (LC50) value of Diethyl sulphate, compound with 2-(heptadec-8-enyl)-4,5-dihydro-1H-imidazole-1-ethanol (1:1) is 0.497 mg/L, with 95 % confidence limits, lower limit of 0.344 mg/L and upper limit of 0.720 mg/L.
Reference
Description of key information
In accordance to OECD 203, the acute toxicity of Diethyl sulphate, compound with 2-(heptadec-8-enyl)-4,5-dihydro-1H-imidazole-1-ethanol (1:1) has been assessed on fish (Cyprinus carpio (Common carp). The 96 hours acute Median Lethal Concentration (LC50) value of Diethyl sulphate, compound with 2-(heptadec-8-enyl)-4,5-dihydro-1H-imidazole-1-ethanol (1:1) is 0.497 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 0.497 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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