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Diss Factsheets
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EC number: 827-581-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Details on sampling:
- After 3 hours, the respiration rate was measured every minute.
- Details on test solutions:
- 5mg, 10mg, 20mg, 40mg and 80mg test substance was added to 200mL test medium respectively. The suspensions were treated with ultrasonic wave for 15min to make the suspension homogeneous.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The activated sludge was obtained from Liede Sewage Treatment Plant of Guangzhou. The sludge was removed any coarse particles and impurities by filtration, then was washed with tap water (repeated for 3 times), after centrifuging the supernatant liquid phase was decanted and the solid material resuspended in deionized water. A small amount of the washed sludge was weighed, thereafter dried and the ratio of wet to dry weight was calculated as 14.4g/L. The final concentration of activated sludge during the test suspension was 1.6g dry material per litre. The sludge was not used on the day of collection, so 50mL synthetic sewage was added to each litre of the activated sludge prepared as described above; the sludge was then aerated at 20°C±2°C until use. Before used the pH was checked
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Test temperature:
- 20.5°C-22.0°C
- Nominal and measured concentrations:
- 10, 20, 40, 80 and 160 mg/L test substance concentration.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dochlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 160 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 160 mg/L
- Nominal / measured:
- nominal
- Results with reference substance (positive control):
- The 3h- EC50 of 3, 5-dichlorophenol was 7.2mg/L within the accepted range of 5 mg/L to 30 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- An OECD 209 Activated Sludge Repiration Inhibition Test on sodium 2-(dodecanoyloxy)propane-1-sulfonate (SLMI) was performed. The 3h-EC50 of the test substance was higher than 160 mg/L and the NOEC may be established at 160 mg/L.
- Executive summary:
An OECD 209 Activated Sludge Repiration Inhibition Test on sodium 2 -(dodecanoyloxy)propane-1 -sulfonate (SLMI) was performed. The 3h-EC50 of the test substance was higher than 160 mg/L and the NOEC may be established at 160 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Letter report of well conducted GLP summary
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Details on test solutions:
- The test substance was weighed directly into the test chambers at concentrations ranging from 62.5 to 1000 mg/L.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Test type:
- not specified
- Total exposure duration:
- 3
- Test temperature:
- 20
- pH:
- 7.2
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a study performed to OECD 209, the test substance (SCI) did not inhibit microorganism activity at the highest concentration tested (1000 mg/L)
Referenceopen allclose all
The results showed that the respiration rates of control at the beginning and end of test were 27.4mg/(L·h) and 26.1mg/(L·h), respectively; the difference between the two sets of control was 2.5%, within 15% of each other. The test solutions with test substance concentrations of 10mg/L, 20mg/L, 40mg/L, 80mg/L, 160mg/L were exposed to activated sludge and respiration rates were 27.7mg/(L·h), 28.4mg/(L·h), 27.2mg/(L·h), 27.6mg/(L·h) and 29.7mg/(L·h). The respiration inhibition rates were -3.4%, -6.3%, -1.5%, -3.2% and -11.0%, respectively. Under 5 concentrations, the test substance had no significant inhibitory effect (<15%) on the respiration rate of activated sludge after the incubation period of three hours and was stimulative of the expiration rates. The results revealed that the 3h-EC50 of the test substance was clearly higher than the nominal concentration of 160 mg/L under the present test conditions and the NOEC may be stabolished at 160 mg/L.
Description of key information
In a key ASRIT study performed to OECD 209, the read-across substance SLMI exhibited a 3h EC50 >160 mg/L. The NOEC may be established at 160 mg/L but in reality the NOEC is likely higher than this (artifact of experimental design)
In a supporting ASRIT study performed to OECD 209 an EC50 of 1000 mg/L was established for the read-across substance SCI.
Although not tested for toxicity to microorganisms, SCMI is expected to exhibit an 3h EC50 value of >1000 mg/L i.e. non-toxic/inhibitory.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 160 mg/L
Additional information
Two studies have been performed to establish the toxicity effect on micro-organisms.
In a study performed to OECD 209 on the read-across substance SCI established a EC50 (3h) of >1000 mg/L.
Another study performed to OECD 209 on SLMI established a NOEC of 160 mg/L (highest concentration tested).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.