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EC number: 271-571-3 | CAS number: 68585-64-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 - 23 Sep 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- no
- Remarks:
- This study was conducted in compliance with Good Laboratory Practice Standards with slight exceptions. For details please refer to the field "Any other information on Material and Methods".
Test material
- Reference substance name:
- Dihydrogen bis[P,P-dioctyl diphosphato(2-)-O'',O''''][hydroxyacetato(2-)-O1,O2]titanate(2-), branched and linear
- EC Number:
- 271-571-3
- EC Name:
- Dihydrogen bis[P,P-dioctyl diphosphato(2-)-O'',O''''][hydroxyacetato(2-)-O1,O2]titanate(2-), branched and linear
- Cas Number:
- 68585-64-8
- Molecular formula:
- not applicable (UVCB substance)
- IUPAC Name:
- ({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)[(2-{[({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)(hydroxy)phosphoryl]oxy}-4-oxo-1,3-dioxa-2-titanacyclopentan-2-yl)oxy]phosphinic acid; {[({bis[(2-ethylhexyl)oxy]phosphanyl}oxy)({[({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)(hydroxy)phosphoryl]oxy})[(2-hydroxyacetyl)oxy]titanio]oxy}({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)phosphinic acid
1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Easton Wastewater Treatment Facility, Easton, Maryland. Collected on September 17, 2018
- Preparation of inoculum for exposure: The sludge was sieved using a 2-mm screen and adjusted to approximately 1000 mg total suspended solids/L with mineral media and then aerated at test temperature until use.
- Initial cell/biomass concentration: 77 × 10^3 colony forming units (CFU)/mL
- Total suspended solids (TSS) measurement performed on the inoculum: 893 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 2470 mL of high grade water, 3 mL calcium chloride solution (2.75%), 3 mL of ferric chloride solution (0.025%), 3 mL of magnesium sulfate solution (2.25%) 30 mL of phosphate buffer (pH 7.4).
- Solubilising agent: Test substance was mixed with silica gel in 2:1 ratio prior to addition to the test system.
- Test temperature: 19.15-21.60 ºC
- pH: 7.4
- pH adjusted: yes
- Suspended solids concentration: A volume of activated sludge soil-amended inoculum to achieve a final TSS concentration of ≤30 mg/L.
TEST SYSTEM
- Culturing apparatus: 4-liter bottles
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: The biodegradation test was started by bubbling CO2-free air through each of the test chambers at a rate of approximately 55 mL per minute. The air entering the chambers was passed through Drierite™ to remove ambient moisture and then through Ascarite® to produce CO2-free air.
- Measuring equipment: Shimadzu Model TOC-VCSH carbon analyzer
- Test performed in closed vessels: yes
- Details of trap for CO2: The CO2 produced from the degradation of organic carbon sources within the test chamber was trapped as potassium carbonate (K2CO3) in the KOH solution and the amount of inorganic carbon in the trapping solution was measured at various intervals during the study, using a Shimadzu Model TOC-VCSH carbon analyzer.
SAMPLING
- Sampling frequency and method: On days 3, 6, 8, 11, 15, 19, 22 and 26, the CO2 trap nearest the test chamber was collected for analysis of inorganic carbon. The two remaining traps remained and a new trap was placed on the end of the series. The two remaining traps were thus one position closer to the test chamber as each new trap was added. On the 28th day of the test, an aliquot of the contents of each test chamber was removed and the pH determined. The contents of all chambers were then acidified by the addition of 1 mL of concentrated hydrochloric acid to drive off inorganic carbonate. All chambers were aerated overnight and then a sample from each test chamber was removed for dissolved organic carbon (DOC) analysis and the trapping solutions closest to the test chambers were analyzed for inorganic carbon.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3
- Reference control: 3
- Toxicity control: 1 (10 mg C/L reference substance and test substance respectively)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 10 mg C/L
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 72.2
- Sampling time:
- 28 d
- Details on results:
- - The toxicity control achieved > 25% degradation by Day 14. Therefore the substance is not considered inhibitory to sludge microorganisms at the concentration tested in this study.
- According to the “Guidance on Information Requirements and Chemical Safety Assessment, Chapter R.7b: Endpoint specific guidance” and to the Guidance “Introduction to the OECD Guidelines for Testing of Chemicals, Section 3: Degradation and Accumulation” the 10-day window is not applicable for UVCB substances.
BOD5 / COD results
- Results with reference substance:
- An average of 98.3% of theoretical CO2 was evolved over the test period. More than 60% biodegradation was achieved on Day 14.
Any other information on results incl. tables
According to the “Guidance on Information Requirements and Chemical Safety Assessment, Chapter R.7b: Endpoint specific guidance” and to the Guidance “Introduction to the OECD Guidelines for Testing of Chemicals, Section 3: Degradation and Accumulation” the 10-day window is not applicable for UVCB substances. Therefore the substance is considered readily biodegradable (72.2% average cumulative biodegradation within 28 d).
Table 1: Dissolved Organic Carbon (DOC) and pH of Test Solutions at Test Termination.
Test Chamber (Nominal Concentration) | DOC* (mg C/L) |
pH |
Control Rep. 1 | 1.4 | 7.57 |
Control Rep. 2 | 1.4 | 7.57 |
Control Rep. 3 | 1.3** | 7.57 |
Sodium Benzoate Rep. 1 (10 mg C/L) | 1.7 | 7.62 |
Sodium Benzoate Rep. 2 (10 mg C/L) | 1.4 | 7.62 |
Sodium Benzoate Rep. 3 (10 mg C/L) | 1.4 | 7.63 |
Test substance rep 1 | 2.3 | 7.53 |
Test substance rep 2 | 2.2 | 7.53 |
Test substance rep 3 | 2.1 | 7.51 |
Toxicity Control (10/10 mg C/L) | 2.1 | 7.56 |
* Samples were filtered (0.45 µm) and acidified prior to analysis.
** Extrapolated from standard calibration curve.
Table 2: Cumulative Percent of Theoretical Carbon Dioxide Evolved.
Date | Day | Blank Control 1 | Blank Control 2 | Blank Control 3 | Sodium Benzoate 1 | Sodium Benzoate 2 | Sodium Benzoate 3 | Test Substance 1 | Test Substance 2 | Test Substance 3 | Toxicity Control |
23. Sep 18 | 3 | NA | NA | NA | 35.7 | 40.4 | 42 | 1.3 | 2.7 | 2.2 | 21.6 |
26. Sep 18 | 6 | NA | NA | NA | 64 | 71.2 | 67.4 | 7 | 9 | 6.6 | 40.2 |
28. Sep 18 | 8 | NA | NA | NA | 76.6 | 81.6 | 77.8 | 15 | 14.1 | 11.2 | 50.4 |
1-Oct-18 | 11 | NA | NA | NA | 84.9 | 89.4 | 85.4 | 28.8 | 28.8 | 25 | 62.7 |
5-Oct-18 | 15 | NA | NA | NA | 89.4 | 93.8 | 90.5 | 41.3 | 49.3 | 42.1 | 73.5 |
9-Oct-18 | 19 | NA | NA | NA | 91.7 | 95.7 | 92.8 | 50.1 | 61.1 | 52.2 | 80.1 |
12-Oct-18 | 22 | NA | NA | NA | 94.1 | 97.9 | 96.4 | 59.4 | 67 | 58.2 | 84 |
16-Oct-18 | 26 | NA | NA | NA | 94.4 | 98.8 | 99.3 | 65.1 | 74.3 | 62.9 | 87.2 |
19-Oct-18 | 29 | NA | NA | NA | 94.1 | 99.9 | 100.8 | 71.3 | 79.3 | 66.1 | 87.8 |
Cumulative Average Day 29 (n=3) | 98.3 | 72.2 | 87.8 | ||||||||
Standard Deviation | 3.6 | 6.6 |
Table 3: Validity criteria for OECD 301.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
13.2 at the end of the test |
yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
Biodegradation on Day 11: 84.9 – 89.4% |
yes |
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC. |
1.3 – 2.7% |
yes |
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
18 mg CO2/L |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
Biodegradation on Day 11: 62.7% |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Interpretation of results:
- readily biodegradable
- Remarks:
- According to the “Guidance on Information Requirements and Chemical Safety Assessment, Chapter R.7b: Endpoint specific guidance” and to the Guidance “Introduction to the OECD Guidelines for Testing of Chemicals, Section 3: Degradation and Accumulation” the 10-day window is not applicable for UVCB substances.
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