Naphthalene-2,6-dicarboxylic acid

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Bioaccumulation Study of Chemicals" described in "About Test Methods for New Chemical Substances" (Kanpogyo Notification No. 5, Yakuhatsu Notification No. 615, and 49 Kikyoku Notification No. 392)

GLP compliance:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch No.of test material: K-599

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: Samples were taken in duplicate 2, 3, 4 and 6 weeks after test start
- Sampling intervals/frequency for test medium samples: Samples were taken in duplicate twice weekly
- Sample storage conditions before analysis: Analysis of the samples was done immediately after sampling
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):

Vehicle:

not specified

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION
Test substance was dissolved in an aqueous solution adjusted to pH 12 with sodium hydroxide. After this, the solution was re-adjusted to pH 7 with hydrochloric acid and filled up with demineralized water to obtain a stock solution of 100 mg/L.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp
- Strain: TFC 840321
- Source: Yatsushiro City, Kumamoto Prefecture Kitamura nursery school
- Age at study initiation: 6 weeks
- Length at study initiation: 9.8 cm (average body lenth)
- Weight at study initiation: 22.42 g (mean body weight)
- Lipid content at test initiation: 4.1% (average lipid content)
- Description of housing/holding area: 100mL glass aquarium
- Feeding during test
- Food type: Carp diet (manufactured by Nippon Blended Manufacturing Co. Ltd.)
- Amount: Approximately 2% equivalent weight of fish weight
- Frequency: Twice a day

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Test temperature:

25 +/- 2°C

Dissolved oxygen:

6.5-7.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 L glass aquarium
- Renewal rate of test solution (frequency/flow rate): 582 L/day
- No. of organisms per vessel: 20

Nominal and measured concentrations:

100 µg/L (first group)
10 µg/L (second group)

Reference substance (positive control):

not specified

Details on estimation of bioconcentration:

The bioconcentration factor (BCF) was calculated according to the following formula:

BCF = (Cfn-Cfb)/Cwn

Cfn: Concentration of the test substance in the sample fish analysis sample
Cwn: Averages of measured concentrations by test water analyzes conducted up to weekday
Cfb: Concentration of the test substance in the fish body (released) in empty test

Conc. / dose:

100 µg/L

Temp.:

25 °C

Type:

BCF

Value:

0.6 L/kg

Basis:

whole body w.w.

Key result

Conc. / dose:

10 µg/L

Temp.:

25 °C

Type:

BCF

Value:

>= 5.2 - <= 7.7 L/kg

Basis:

whole body w.w.

Validity criteria fulfilled:

not specified

Conclusions:

The maximum BCF of the test substance to the carp was 7.7 L/kg, corresponding to a log BCF of 0.89.

Key value for chemical safety assessment

BCF (aquatic species):

7.7 L/kg ww