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EC number: 221-297-5 | CAS number: 3058-38-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- purity of test substance; acclimation period, details on test animals/environmental conditions, detailed test procedure and results not reported
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- yes
- Remarks:
- purity of test substance; acclimation period, details on test animals/environmental conditions, detailed test procedure and results not reported
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Remarks:
- pre-GLP
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- At the time of study completion (1976), the LLNA was not available.
Test material
- Reference substance name:
- 2,4,6-trinitrobenzene-1,3,5-triamine
- EC Number:
- 221-297-5
- EC Name:
- 2,4,6-trinitrobenzene-1,3,5-triamine
- Cas Number:
- 3058-38-6
- Molecular formula:
- C6H6N6O6
- IUPAC Name:
- trinitrobenzene-1,3,5-triamine
- Test material form:
- not specified
- Details on test material:
- - Name of test material as cited in the report: TATB ; 1,3,5-triamino-2,4,6-trinitrobenzene
- Lot/batch No.of test material: B-318
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- male
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 300-400 g
- Housing: Animals were housed individually
- Diet: Commercial laboratory ration supplemented on alternate days with fresh lettuce or cabbage, ad libitum
Study design: in vivo (non-LLNA)
Induction
- Route:
- intradermal
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 1%
- Concentration / amount:
- 0.1%
1st injection of 0.05 mL; other 9 injections of 0.1 mL (10 injections) - Day(s)/duration:
- 2 weeks
Challenge
- No.:
- #1
- Route:
- intradermal
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 1%
- Concentration / amount:
- 0.05 mL
- Day(s)/duration:
- 24 hours
- No. of animals per dose:
- 10 animals
- Details on study design:
- PRE-TREATMENT: Before each injection, the test site was clipped free of hair with electric small-animal clippers.
INDUCTION EXPOSURE
Test substance was administered in a series of ten "sensitising" injections into the lower back and flanks of the guinea pigs. Injections were made randomly over the test area every other day using tuberculin syringe. The volume of the first injection was 0.05 mL; the other nine injections were each 0.1 mL. Twenty-four hours after each injection, the resulting reaction was scored for redness, height, and diameter. Redness and height were scored as described by Draize method; diameters of reactions were measured in millimeters using micrometer calipers.
CHALLENGE EXPOSURE
Two weeks after administration of the tenth sensitising injection, the lower back and flanks of each experimental guinea pig were clipped free of hair, and a challenge injection of 0.05 mL was administered. Each animal's reactions were graded 24 hours later and compared with those from the sensitizing injections. - Challenge controls:
- None
- Positive control substance(s):
- not specified
Results and discussion
- Positive control results:
- None
In vivo (non-LLNA)
Resultsopen allclose all
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 0.1%
- No. with + reactions:
- 0
- Clinical observations:
- None
- Remarks on result:
- no indication of skin sensitisation
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 0.1%
- No. with + reactions:
- 0
- Clinical observations:
- None
- Remarks on result:
- no indication of skin sensitisation
- Group:
- positive control
- Remarks on result:
- not measured/tested
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the test conditions, the test substance is not classified as skin sensitiser, therefore it is not classified according to the annex I of the Regulation EC No. 1272/2008 (CLP) and to the GHS.
- Executive summary:
In a skin sensitisation study, the test substance was administered to group of male Hartley guinea pigs as induction and challenge phase. Test substance was diluted to a concentration of 0.1% with a 1% solution of carboxymethylcellulose. The control group received the 1% solution or of carboxymethylcellulose alone.
Induction phase: Test substance was administered in a series of ten "sensitising" injections into the lower back and flanks of the guinea pigs. The volume of the first injection was 0.05 mL; the other nine injections were each 0.1mL. Twenty-four hours after each injection, the resulting reaction was scored for redness, height, and diameter.
Challenge phase: Two weeks after administration of the tenth sensitising injection, the lower back and flanks of each experimental guinea pig were clipped free of hair, and a challenge injection of 0.05 mL was administered. Each animal's reactions were graded 24 hours later and compared with those from the sensitizing injections.
No skin sensitization reactions were observed in the treated animals.
Under the test conditions, the test substance is not classified as skin sensitiser, therefore it is not classified according to the annex I of the Regulation EC No. 1272/2008 (CLP) and to the GHS.
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