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EC number: 200-641-8 | CAS number: 67-03-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 October 1992 to 16 November 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Composite samples (about 150 mL per test concentration and control) were drawn by mixing identical volumes of the test solutions taken from the approximate centre of the test vessels.
- They were taken immediately before exposure and after 48 hours exposure and kept at -18 to -22 °C until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- 100.3 mg test material was mixed and made up to 1000 mL with water.
- Calculated amounts of the stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. The algae were then transferredTEST SYSTEM
- Test vessel: 100 mL Erlenmeyer flasks, stoppered with aluminium caps, on Lab-shaker
- Material, size, headspace, fill volume: 50 mL
- Initial cells density: 9400 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Composition according to the guideline
- Intervals of water quality measurement: Temperature was continuously measured and maintained at 23 ± 1°C. pH was measured at 0 and 72 h exposure.
OTHER TEST CONDITIONS
- Photoperiod: Continuous illumination
- Light intensity and quality: cold white fluorescent light, 109 µE/m^2 sec (approx. 8000 lux)
EFFECT PARAMETERS MEASURED:
- Cell densities were measured at 24, 48 and 72 hours exposure on a “TOA” cell counter.
into the flasks. The test material was homogeneously distributed in the test vessels at all test times and test concentrations. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Source (laboratory, culture collection): Collection of algae cultures, Pflanzenphysiologisches Institut, University, Nikolausberger Weg 18, 0-3400 Göttingen, Germany
- Method of cultivation: cultured according to OECD 201
ACCLIMATION
- Acclimation period: 3 days under test conditions - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 ± 1 °C
- pH:
- 6.6 - 8.4
- Nominal and measured concentrations:
- Nominal: 1.23, 3.7, 11, 33 and 100 mg/L
Measured (0 hours): 1.2, 3.6, 10.4, 31.5 and 96.5 mg/L
Measured (72 hours): 1.3, 3.3, 10.1, 30.9 and 94.2 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL Erlenmeyer flasks, stoppered with aluminium caps, on Lab-shaker
- Material, size, headspace, fill volume: 50 mL
- Initial cells density: 9400 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Composition according to the guideline
- Intervals of water quality measurement: Temperature was continuously measured and maintained at 23 ± 1°C. pH was measured at 0 and 72 h exposure.
OTHER TEST CONDITIONS
- Photoperiod: Continuous illumination
- Light intensity and quality: cold white fluorescent light, 109 µE/m^2 sec (approx. 8000 lux)
EFFECT PARAMETERS MEASURED:
- Cell densities were measured at 24, 48 and 72 hours exposure on a “TOA” cell counter. - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Reported statistics and error estimates:
- The EbC-50 values were calculated according to the maximum likelihood method, probit model (Mc Cullagh, P., Nelder, J.A., 1983: Generalised linear models, Chapman & Hall, London)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of this study the EbC50 (0-72 h) was >100 mg/L and the NOEbC was 100 mg/L.
- Executive summary:
The short-term toxicity of the test material to aquatic algae was investigated in accordance with the standardised guideline OECD 201, under GLP conditions.
Green algae (Scenedesmus subspicatus) was exposed to the test material at nominal concentrations of 1.23, 3.7, 11, 33 and 100 mg/L for 72 hours. Each test concentration was tested in triplicate and 6 control flasks were also used. Cell densities were measured at 24, 48 and 72 hours using a cell counter.
The test material concentrations were analytically determined using UV-Vis and were shown to be between 89 - 106 % of the nominal values.
Under the conditions of this study the EbC50 (0-72 h) was >100 mg/L and the NOEbC was 100 mg/L.
Reference
Table 1: Summary of Results
Nominal Concentration (mg/L) |
Mean Cell Density (cells/mL*10000) |
Inhibition of growth(%) |
||
24 hours |
48 hours |
72 hours |
0-72 h |
|
Blank |
3.3 |
17.3 |
85.7 |
0.0 |
1.23 |
3.3 |
16.9 |
123.0 |
0.0 |
3.7 |
3.3 |
17.6 |
129.0 |
0.0 |
11 |
3.0 |
16.9 |
112.2 |
0.0 |
33 |
3.7 |
17.8 |
114.8 |
0.0 |
100 |
3.8 |
12.0 |
74.2 |
17.3 |
Description of key information
Under the conditions of this study the EbC50 (0-72 h) was >100 mg/L and the NOEbC was 100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
The short-term toxicity of the test material to aquatic algae was investigated in accordance with the standardised guideline OECD 201, under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).
Green algae (Scenedesmus subspicatus) was exposed to the test material at nominal concentrations of 1.23, 3.7, 11, 33 and 100 mg/L for 72 hours. Each test concentration was tested in triplicate and 6 control flasks were also used. Cell densities were measured at 24, 48 and 72 hours using a cell counter.
The test material concentrations were analytically determined using UV-Vis and were shown to be between 89 - 106 % of the nominal values.
Under the conditions of this study the EbC50 (0-72 h) was >100 mg/L and the NOEbC was 100 mg/L.
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