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EC number: 947-853-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Biodegradation in water: screening test, toxicity control is used to derive effect concentration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Principles of method if other than guideline:
- From biodegradation in water: screening test, toxicity control is used to derive effect concentration
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany
- Preparation of inoculum for exposure: kept aerobic and filtered through a coarse filter paper.
- Storage length: until use in the test, at least 1 h
- Concentration of sludge: 1 mL/L - Test type:
- other: aerobic
- Water media type:
- other: mineral medium
- Limit test:
- no
- Total exposure duration:
- 28 d
- Test temperature:
- 20 (± 1 °C)
- Details on test conditions:
- TEST CONDITIONS
- Composition of medium: ( a ) KH 2 PO 4 8.50 g/L
K2HPO4 21.75 g/L
Na2HPO4 26.64 g/L
NH4Cl 0.51 g/L
( b ) CaCl2 x 2H2O 36.41 g/L
( c ) MgSO4 x 7H2O 22.50 g/L
( d ) FeCl3 x 6H2O 0.25 g/L.
The mineral medium applied in the test contained 1 mL/L of the mineral stock solution a–d, respectively. The mineral medium was strongly aerated for 20 minutes and allowed to stand for 20 h at test temperature.
- Test temperature: 20 °C (± 1 °C)
- Aeration of dilution water: yes
- Continuous darkness: yes
SAMPLING
- Sampling frequency: day 3*, 6, 10*, 14, 21*, and 28 (* without toxicity control) using an O 2 electrode.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 16 vessels containing only inoculum
- Toxicity control: 8 vessels containing test item (40 mg/L), reference item (2 mg/L) and inoculum
STATISTICAL METHODS:
Theoretical Oxygen Demand (ThOD):
The Theoretical Oxygen Demand (ThOD) was calculated on the basis of the sum formula of the test and reference item considering complete nitrification.
Biochemical Oxygen Demand (BOD):
The Biochemical Oxygen Demand (BOD) was calculated on the basis of the test raw data by BOD [mg/mg] = mg O2 uptake corrected by blank per mg test item.
The percent degradation was calculated according to the following formula:
D t = [(C t –C b ) / ThOD] x 100
D t : degradation (%) at time t;
C t : mean oxygen consumption (mg/L) in the test suspension at time t;
C b : mean oxygen consumption (mg/L) in the blanks at time t;
ThOD: Theoretical oxygen demand of the test suspension (mg/L). - Reference substance (positive control):
- yes
- Remarks:
- sodium benzoate
- Key result
- Duration:
- 14 d
- Dose descriptor:
- other: % degradation (O2 consumption)
- Effect conc.:
- 26 other: %
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Remarks on result:
- other: O2 consumption after 14 days
- Details on results:
- - Blank controls oxygen uptake rate: O2 consumption/ % degradation after 14 d 96.9% within the procedural control.
- Results with reference substance (positive control):
- The biodegradation of sodium benzoate was 87.4% after 28 days.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- In a study concucted according to OECD guideline 301D 1992, the toxicity of hydrogenated tallow/nortallow based IQAC to microorganisms was determined. The test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 40 mg/L.
- Executive summary:
The toxicity of hydrogenated tallow/nortallow based IQAC to microorganisms was investigated during a ready biodegradation study according to EU Method C.4-D (2008) and OECD guideline 301D (1992), Closed Bottle Test over a period of 28 days and using an inoculum obtained from activated sludge from a predominantly domestic sewage treatment plant.
Inoculum blank, procedural/functional control with the reference substance Sodium benzoate and a toxicity control with 20 and 40 mg/L test item and 2 mg/L reference item Sodium benzoate were performed.
The reference item degraded normally (87.4 % by within 28 days).
The biodegradation of the item mixture in the toxicity control was found to be 75.5 % after 14 days of incubation. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 40 mg/L, as the biodegradation in the toxicity control was higher than 25% within 14 days of incubation.
Reference
Description of key information
- study conducted according to OECD guideline 301 D (Closed Bottle Test), domestic sewage treatment plant sludge was incubated with 40 mg/L test item and 2 mg/L reference item, after 14 days degradation was > 25%, thus the substance is not inhibitory.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 40 mg/L
Additional information
The biodegradation of hydrogenated tallow/nortallow based IQAC, aqueous dispersion (5% ) was investigated over a 28-day period in a Closed Bottle Test according to OECD guideline 301 D (1992). The test medium was inoculated with activated sludge from a sewage treatment plant mainly fed with municipal wastewater. The rate of degradation was monitored by measuring the oxygen consumption over the 28-d period.
The test item was tested with a concentration of 20 and 40 mg/L in duplicates, corresponding to a carbon content (ThOC) of 2.83 and 5.66 mg C/L in the test vessels.
Degradation is followed by analysis of dissolved oxygen over a 28-day period. The amount of oxygen taken up by the microbial population during biodegradation of the test item (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD.
The 10 % level (beginning of biodegradation of the test item) was reached within an adaptation phase of 3 days for the procedural control. The percentage of degradation of the functional control reached the pass level of 60 % after 3 days.
The highest mean biodegradation rate of hydrogenated tallow/nortallow based IQAC aqueous dispersion (5 %) within the 28 days of incubation in the static test was found to be 6 % (day 6) in the assays with 20 mg/L and 3 % (day 21) in the assays with 40 mg/L. Thus, no biodegradation within a 10-day-window could be obtained. The biodegradation of the item mixture in the toxicity control was found to be 26 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item hydrogenated tallow/nortallow based IQAC, aqueous dispersion (5 %) can be identified as non-toxic in a ready biodegradability test. The reference item sodium benzoate was degraded to 97 % within the first 14 days, which was the highest degradation rate found within the test duration. In conclusion, according to the guideline, the test item hydrogenated tallow/nortallow based IQAC aqueous dispersion (5 %) must be identified as not readily biodegradable under the chosen test conditions.
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