Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-942-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling schedule: Control and test concentration at 0 and 72 hours
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM:
- Source : Strain of the test species obtained from 'The Collection of Algal Cultures' of the Institute of Plant Physiology at the University of Göttingen (Germany).
- Maintenance and Acclimatisation : Exponentially-growing stock cultures are maintained in the test facility under constant temperature conditions (23 +/- 2 °C) at a light intensity in the range 60 – 120 μE. x m-2 x s-1 (measured in the range 400 to 700 nm using a spherical quantum flux meter). The nutrient medium (according to BRINGMANN & KÜHN (1977) is renewed once a week. Cell density measurements are made using a microcell counter.
- Preparation of pre cultures : Pre cultures were set up three days before the start of a test. They were grown under identical exposure conditions as the stock cultures, except from the use of a different growth medium.
- Test cultures : The algal inocula for the test were taken from an exponentially growing pre culture and were mixed with the nutrient medium to make up to a final cell density of about 5000 cells per millilitre in the test medium. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 1.3 °dH, corresponding to 22.5 mg/L CaCO3
- Test temperature:
- No data
- pH:
- pH range: 8.3/7.9 (at test start, after 72 h)
- Dissolved oxygen:
- No data
- Salinity:
- n.a.
- Nominal and measured concentrations:
- Nominal concentrations: 0.13, 0.25, 0.5 and 1 mg/L
Measured concentrations: 0.073, 0.132, 0.307 and 0.730 mg/L (HPLC mean value [mg/L] after 72 h)
Measured concentration of 1.0 mg/L without algal inoculum after 72 hours: 0.894 mg/L - Details on test conditions:
- PRETREATMENT OF TEST ITEM:
- 1.4 mg of the test item were added into 1 L of dilution water
- treated for 60 seconds at 8000 rpm with an ultra turrax, afterwards stirred for 24 h on a magnetic stirrer
TEST SYSTEM:
- test vessels: 300 mL Erlenmeyer flasks with cotton stoppers
- culturing apparatus: temperature 21 to 25 °C, continuous uniform illumination (spectral range 400 to 700 nm)
- light intensity: 60 -120 µE x mE-2 x sE-1 or an equivalent range of 4000-8000 lx
- test cultures: final cell density 5000 cells/mL
- test concentration: 4 (0.13, 0.25, 0.50 1.0 mg/L) plus 1 control
- replicates: 3 (per concentration), 6 (per control)
- method of administration: stock solution
- test parameter: growth and growth rate
- chemical analysis: HPLC + MSD-detection
MONITORING DATA (concerning the highest test concentration):
- cell density measurements of growth (daily)
- analysis: twice (at test start, after 72 h) - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.282 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.235-0.321 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.002 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.905-1.145 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.163 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.189 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 0.081-0.267 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.488 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 0.382-0.646 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.163 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- Recovery rates ranged from 77.5-83.1 % of nominal values at 0 hours, and from 52.6-89.4 % of nominal values at 72 hours, respectively.
- Validity criteria fulfilled:
- yes
- Remarks:
- (- The factor of biomass parameter 80.8 >16; - The mean of the replicate coefficient of variation in the section-by-section growth 8.0 % <35 %; - The coefficient of variation of the mean specific growth rate replicates in the control 0.9 % <7 %.)
- Conclusions:
- An ErC50 of 1.002 mg/L was determined and a NOEC[r] of 0.163 mg/L was calculated after 72 hrs. Recovery rates ranged from 77.5 - 83.1 % of nominal values at 0 hours, and from 52.6 - 89.4 % of nominal values at 72 hours, respectively.
- Executive summary:
The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 3 'Algal inhibition test' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 201 'Alga, Growth Inhibition Test'. Exponentially growing algal cells of the planktonic freshwater algal species Desmodesmus subspicatus were exposed for a period of 72 hours to a range of concentrations, nominally 0.13, 0.25, 0.5 and 1.0 mg/L of the substance dissolved in water. Auxiliaries used to prepare the test media were an ultra turrax and a magnetic stirrer. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth (index b) and growth rate (index r), relative to control cultures grown under identical conditions. Growth and growth rates were used to calculate a No Observed Effect Concentration and a Lowest Observed Effect Concentration according to DUNNETT (1955, 1964). An ErC50 of 1.002 mg/L was determined and a NOEC[r] of 0.163 mg/L was calculated after 72 hrs. Recovery rates ranged from 77.5 - 83.1 % of nominal values at 0 hours, and from 52.6 - 89.4 % of nominal values at 72 hours, respectively.
This toxicity study is classified as acceptable and satisfies the guideline requirements for the Algae study.
Reference
Description of key information
An ErC50 of 1.002 mg/L was determined and a NOEC[r] of 0.163 mg/L was calculated after 72 hrs. Recovery rates ranged from 77.5 - 83.1 % of nominal values at 0 hour, and from 52.6 - 89.4 % of nominal values at 72 hours, respectively (Weyers, 2007).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.002 mg/L
- EC10 or NOEC for freshwater algae:
- 0.163 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.