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Diss Factsheets
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EC number: 205-324-8 | CAS number: 138-32-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study was conducted according to OECD guideline 201 as well as in compliance with GLP with acceptable restrictions. Chemical analyses were not performed.
- Justification for type of information:
- Direct read-across from C16 TMAC to Cetrimonium chloride is claimed as valid because they are both trimmoniums sharing a common alkyl chain length n=16. C16 TMAC is also known as the cetrimonium ion, exhibts properties and effects consistent with a category of quaternary ammonium compounds known as the alkyl trimoniums. C16 TMAC is commonly known as the cetrimonium ion. In pure form QACs also contain an anion such as chloride, bromide or methosulfate. When in solution the substances will be dissociated and the anion the QACs were once associated with will be irrelevant.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- KH2PO4 and NaHCO3 concentrations were 160 and 100 mg/L
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Substance type: Quaternary ammonium compound; surfactant- Physical state: Solid- Analytical purity: >97%- Lot/batch No.: G4 2892- Stability under test conditions: stable- Storage condition of test material: ambient conditions in the dark
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 1 g/L was prepared in deionised water. Test solutions were prepared by dilution of the stock solution in OECD medium.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Common name: Selenastrum capricornutum ATCC 22662- Source (laboratory, culture collection): ATCC- Age of inoculum (at test initiation): exponentially growing - Method of cultivation: identical as during the test
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- Standard OECD medium
- Test temperature:
- 22.5 - 23°C
- pH:
- 6.8 - 7.6
- Dissolved oxygen:
- -
- Salinity:
- Standard OECD medium
- Nominal and measured concentrations:
- Nominal: 0, 0.01, 0.02, 0.04, 0.08 and 0.16
- Details on test conditions:
- The culturing apparatus was a cabinet in which the temperature was maintained between 22 and 24°C, and continuous uniform illumination was provided in the spectral range of 400 to 700 m. The light intensity is in the range of 6000 to 10000 lux which is obtained by using seven 30 W fluorescenr lamps (colour temperature of approximately 4300 K), at a distance of 0.35 m from the algal culture. The culture flasks were rotated continuously at 100 rpm to prevent sedimentation of the algae.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate 99.5% purity lot H0234 JT baker
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.08 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.07 - 0.08 mg/L 95% confidence limits
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.05 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 0.04 - 0.05 mg/L 95% confidence limits
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.04 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth and growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.047 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- The absorbance measurement data of potassium dichromate included in the report. From results, an ErC50 and EbC50 (0-72 h) of potassiumdichromate of 0.57 and 0.23 mg/L, respectively, was calculated. These values of the reference compound were in accordance with data of an international interlaboratory test showing that the test organisms have not changed significantly and that the slight modification of the mineral salts medium do not influence the result.
- Reported statistics and error estimates:
The EC50 values were computed from the best fitted line (least square method) through the points given by the probit of the percentage inhibition and the logarithm of the concentration of the test substance. The EC50 value calculated with the area under the growth curve is termed EbC50 (0-72 h) whereas the EC value calculated with the specific growth rate is termed ErC50. Confidence limits were computed based on Fieller's theorem. All computations were performed with a programme (Akzo programme, Algal version 1.0) using the software package SAS.- Validity criteria fulfilled:
- yes
- Remarks:
- but no chemical analysis was performed
- Conclusions:
- Under the study conditions, the EbC50 (0 -72 h) and ErC50 of test substance for Selenastrum capricornutum were 0.05 and 0.08 mg a.i./L, respectively. The NOEC for effect on algal growth and growth rate was determined to be 0.04 mg/L.
Based on read-across from TMAC C16, and using the category approach for alkyl trimmoniums, this result is deemed valid for cetrimonium chloride. - Executive summary:
The NOEC for effect on algal growth and growth rate was determined to be 0.04 mg a.i./L and the ErC10 value was 0.047 mg a.i./L. This ErC10 with a value of 0.047 mg/L is just a bit higher than the NOEC. The CV is now much higher than the max of 35% indicating that this study is based on the 2008 criteria not valid. Also here there is only one value which gives a partial response indicating that the test concentrations chosen were too widely distributed.
Reference
Description of key information
In a study using the read-across source, C12 -18 TMAC, under the study conditions, the EbC50 (0 -72 h) and ErC50 of test substance for Selenastrum capricornutum were 0.05 and 0.08 mg a.i./L, respectively. The NOEC for effect on algal growth and growth rate was determined to be 0.04 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.08 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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