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Reaction mass of Chromate(1-), [2-(3-chlorophenyl)-2,4-dihydro-4-[[2-hydroxy-5-(methylsulfonyl)phenyl]azo]-5-methyl-3H-pyrazol-3-onato(2-)][methyl [7-hydroxy-8-[[2-hydroxy-5-(methylsulfonyl)phenyl]azo]-1-naphthalenyl]carbamato(2-)]-, sodium and sodium bis[2-(3-chlorophenyl)-2,4-dihydro-4-[[2-hydroxy-5-mesylphenyl]azo]-5-methyl-3H-pyrazol-3-onato(2-)]chromate(1-) and sodium bis[methyl [7-hydroxy-8-[[2-hydroxy-5-mesylphenyl]azo]-1-naphthyl]carbamato(2-)]chromate(1-)
EC number: 915-704-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-10-10 to 2016-11-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992-07-17
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Version / remarks:
- 2008-05-30
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of Chromate(1-), [2-(3-chlorophenyl)-2,4-dihydro-4-[[2-hydroxy-5-(methylsulfonyl)phenyl]azo]-5-methyl-3H-pyrazol-3-onato(2-)][methyl [7-hydroxy-8-[[2-hydroxy-5-(methylsulfonyl)phenyl]azo]-1-naphthalenyl]carbamato(2-)]-, sodium and sodium bis[2-(3-chlorophenyl)-2,4-dihydro-4-[[2-hydroxy-5-mesylphenyl]azo]-5-methyl-3H-pyrazol-3-onato(2-)]chromate(1-) and sodium bis[methyl [7-hydroxy-8-[[2-hydroxy-5-mesylphenyl]azo]-1-naphthyl]carbamato(2-)]chromate(1-)
- EC Number:
- 915-704-1
- Molecular formula:
- C36H28ClCrN7O10S2.Na / C34H26Cl2CrN8O8S2.Na / C38H30CrN6O12S2.Na
- IUPAC Name:
- Reaction mass of Chromate(1-), [2-(3-chlorophenyl)-2,4-dihydro-4-[[2-hydroxy-5-(methylsulfonyl)phenyl]azo]-5-methyl-3H-pyrazol-3-onato(2-)][methyl [7-hydroxy-8-[[2-hydroxy-5-(methylsulfonyl)phenyl]azo]-1-naphthalenyl]carbamato(2-)]-, sodium and sodium bis[2-(3-chlorophenyl)-2,4-dihydro-4-[[2-hydroxy-5-mesylphenyl]azo]-5-methyl-3H-pyrazol-3-onato(2-)]chromate(1-) and sodium bis[methyl [7-hydroxy-8-[[2-hydroxy-5-mesylphenyl]azo]-1-naphthyl]carbamato(2-)]chromate(1-)
- Test material form:
- solid
- Details on test material:
- Appearance
- physical state. solid
- color: brown
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany. The inoculum was collected on 19 October 2016 from the aeration tank of the plant.
- Preparation of inoculum for exposure: A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. To reduce the content of inorganic carbon in the blank controls the activated sludge was aerated with carbon dioxide free air for about 24 hours at 22 ± 2 °C.
- Pretreatment: At the day of exposure the suspension was washed one time with drinking water. Therefore the aeration was stopped and the sludge was allowed to settle. After settling the supernatant was discarded and the remaining sludge suspension was filled up with drinking water.
- Concentration of sludge: 6.0 g/L dry weight - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
- Initial conc.:
- ca. 44 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The used mineral medium complies with the test guideline OECD 301B. It was prepared as follows:
Solution A: KH2PO4 8.50 g, K2HPO4 21.75 g, Na2HPO4 x 2 H2O 33.40 g, NH4Cl 0.50 g; the compounds were dissolved with deionized water to 1000 mL; the pH value was adjusted to 7.4.
Solution B: CaCl2 x 2 H2O 36.40 g; the compound was dissolved with deionized water to 1000 mL.
Solution C: MgSO4 x 7 H2O 22.50 g; the compound was dissolved with deionized water to 1000 mL.
Solution D: FeCl3 x 6 H2O 0.25 g; Tte compound was dissolved with deionized water to 1000 mL.
15 mL solution A, 1.5 mL solution B, 1.5 mL solution C and 1.5 mL solution D was used for the preparation of the test assays.
- Test temperature: 22 ± 2 °C
- pH: 7.4 ± 0.2
- pH adjusted: yes
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Culturing apparatus: 2 L incubation bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: The incubation bottles were stirred on magnetic stirrers; the aeration was performed with carbon dioxide free air at a flow of approximately 800 mL per hour
- Measuring equipment: The bottles were connected to two serial scrubbing bottles (total volume 250 mL) filled with 100 mL 0.05 mol sodium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes.
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: s.a.
SAMPLING
- Sampling frequency: twice a week
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no
- Other: Inhibition control, positive control (reference substance, aniline)
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Degree of biodegradation of the reference substance after 14 days: 85 % CO2/ThCO2
Any other information on results incl. tables
Table 1: Measurement of Total Inorganic Carbon, measured data: TIC [mg/L]
Day |
|
BC NaOH |
BC1 |
BC2 |
RS |
IH |
TS1 |
TS2 |
0 |
a |
0.9 |
- |
- |
- |
- |
- |
- |
b |
0.8 |
|||||||
1 |
a |
0.7 |
6.7 |
7.0 |
6.8 |
6.9 |
5.1 |
7.6 |
b |
0.8 |
6.9 |
6.8 |
6.8 |
6.8 |
5.0 |
7.6 |
|
4 |
a |
0.9 |
24.3 |
25.1 |
91.2 |
109 |
17.8 |
23.5 |
b |
1.0 |
24.2 |
25.1 |
91.2 |
109 |
17.5 |
23.2 |
|
7 |
a |
0.8 |
30.5 |
30.0 |
128 |
115 |
23.8 |
21.7 |
b |
0.7 |
29.7 |
29.4 |
129 |
116 |
23.6 |
21.3 |
|
11 |
a |
0.9 |
37.4 |
35.6 |
104 |
73.3 |
26.7 |
27.1 |
b |
0.8 |
36.5 |
35.1 |
104 |
73.0 |
26.2 |
26.8 |
|
14 |
a |
0.8 |
24.6 |
25.4 |
46.4 |
31.0 |
20.7 |
18.8 |
b |
0.9 |
23.9 |
25.1 |
46.1 |
31.0 |
20.5 |
18.9 |
|
18 |
a |
1.2 |
26.7 |
25.9 |
38.2 |
28.2 |
23.0 |
21.3 |
b |
1.2 |
26.1 |
25.4 |
37.8 |
27.8 |
23.1 |
21.3 |
|
22 |
a |
0.8 |
15.2 |
17.7 |
31.2 |
21.6 |
24.7 |
22.0 |
b |
0.8 |
15.1 |
17.7 |
31.1 |
21.5 |
24.8 |
22.0 |
|
25 |
a |
0.9 |
14.5 |
15.3 |
26.0 |
17.5 |
19.1 |
19.4 |
b |
0.8 |
14.7 |
15.1 |
26.0 |
17.4 |
19.0 |
19.2 |
|
27 |
a |
0.7 |
10.4 |
12.4 |
17.3 |
12.7 |
14.8 |
15.3 |
b |
0.8 |
10.2 |
12.3 |
17.6 |
12.6 |
14.6 |
15.4 |
|
28 |
a |
0.8 |
7.1 |
7.4 |
11.0 |
8.1 |
8.9 |
10.3 |
b |
0.7 |
7.0 |
7.3 |
11.2 |
8.2 |
8.7 |
10.0 |
|
flask 1 29 |
a |
|
15.2 |
17.6 |
15.2 |
15.8 |
16.3 |
11.1 |
b |
15.1 |
17.3 |
15.1 |
16.0 |
16.4 |
11.4 |
||
flask 2 29 |
a |
4.6 |
3.7 |
4.1 |
4.7 |
4.6 |
4.5 |
|
b |
4.5 |
3.7 |
4.0 |
4.5 |
4.6 |
4.5 |
The NaOH blank value is determined on filling the absorption vessels.
The measured values, for instance applies on day 0 for the first and the second sampling.
The NaOH blank value was subtracted from each test vessel.
Table 2: Produced carbon dioxide amount in the test vessels
[mg CO2/test vessel] |
mg CO2 added up after subtraction of the mean value of the blank controls. |
||||||||
Duration of exposure [days] |
BC mv |
RS |
IH |
TS1 |
TS2 |
RS |
IH |
TS1 |
TS2 |
1 |
2.2 |
2.2 |
2.2 |
1.5 |
2.5 |
0.0 |
0.0 |
-0.7 |
0.3 |
4 |
8.7 |
33.2 |
39.7 |
6.2 |
8.3 |
24.5 |
31.0 |
-3.2 |
-0.1 |
7 |
10.7 |
46.9 |
42.1 |
8.4 |
7.6 |
60.7 |
62.4 |
-5.5 |
-3.2 |
11 |
12.9 |
37.8 |
26.5 |
9.4 |
9.5 |
85.6 |
76.0 |
-9.0 |
-6.6 |
14 |
8.8 |
16.7 |
11.1 |
7.3 |
6.6 |
93.5 |
78.3 |
-10.5 |
-8.8 |
18 |
9.2 |
13.6 |
10.0 |
8.1 |
7.5 |
97.9 |
79.1 |
-11.6 |
-10.5 |
22 |
5.7 |
11.1 |
7.6 |
8.8 |
7.8 |
103.3 |
81.0 |
-8.5 |
-8.4 |
25 |
5.0 |
9.1 |
6.0 |
6.6 |
6.6 |
107.4 |
82.0 |
-6.9 |
-6.8 |
27 |
3.9 |
6.1 |
4.3 |
5.1 |
5.3 |
109.6 |
82.4 |
-5.7 |
-5.4 |
28 |
2.3 |
3.8 |
2.7 |
2.9 |
3.4 |
110.7 |
82.9 |
-4.9 |
-6.0 |
29 |
6.9 |
6.5 |
7.0 |
7.1 |
5.2 |
|
|
|
|
mg CO2 in the test assay = (TICmv x 44 x 100) / 12 x 1000
TICmv = measured TIC mean value after subtraction of the NaOH blank value
12 = atomic weight of carbon
44 = molecular mass of carbon dioxide
100/1000 = conversion into 100 mL absorption solution
mg CO2 added up = CO2mv - CO2mv BC + CO2pd
CO2mv = CO2 amount in the test assay at sampling day
CO2mv BC = CO2 amount in the blank control at sampling day
CO2pd = CO2 amount in the test assay at previous sampling day
Table 3: Calculation of degree of biodegradation, Theoretical Carbon dioxide [mg/test assay]:
RS : 109.6 |
IH : 220.2 |
TS1 : 111.1 |
TS2 : 109.9 |
Table 4: Degree of Biodegradation [% CO2/ThCO2]
Test duration [days] |
RS |
IH |
TS1 |
TS2 |
TS mv |
0 |
0 |
0 |
1 |
0 |
0 |
1 |
0 |
0 |
-1 |
0 |
-1 |
4 |
22 |
14 |
-3 |
0 |
-2 |
7 |
55 |
28 |
-5 |
-3 |
-4 |
11 |
78 |
35 |
-8 |
-6 |
-7 |
14 |
85 |
36 |
-9 |
-8 |
-9 |
18 |
89 |
36 |
-10 |
-10 |
-10 |
22 |
94 |
37 |
-8 |
-8 |
-8 |
25 |
98 |
37 |
-6 |
-6 |
-6 |
27 |
100 |
37 |
-5 |
-5 |
-5 |
28 |
101 |
38 |
-4 |
-5 |
-5 |
[% CO2/ThCO2] = (CO2Ta / ThCO2Ta) x 100
CO2Ta = CO2 amount at sampling day
ThCO2Ta = Theoretical CO2 amount in the test assay
Table 5: Measurement of Dissolved Organic Carbon
Measured values [mg/L], determination of DOC after centrifugation
|
[mg/L DOC] |
||||||||
BC1 |
BC2 |
BC mv |
RS |
IH |
TS1 |
TS2 |
TS mv |
||
At begin of exposure |
a |
0.5 |
0.4 |
0.5 |
18.9 |
- |
- |
- |
|
b |
0.5 |
0.4 |
19.0 |
- |
- |
- |
|||
At the end of exposure |
a |
1.0 |
0.8 |
0.9 |
0.9 |
- |
- |
- |
|
b |
1.0 |
0.9 |
0.9 |
- |
- |
- |
|||
Degradation of DOC [%] |
100 |
- |
- |
- |
- |
Degradation of DOC [%] = {1 - [(CTAt - CBCt) / (CTA0 - CBC0)]} x 100
CTAt= average DOC conc. of the reference substance assays; current values
CBCt= average DOC conc. of the blank control assays; current values
CTA0= average DOC conc. of the reference substance assays at day 0
CBC0= average DOC conc. of the blank control assays at day 0
List of abrevations:
BC: blank control assay
DIC: Dissolved Inorganic Carbon
DOC: dissolved organic carbon equiv.: equivalent
IH: inhibition control assay
Mv: mean value
RS: reference substance assay
TC: total carbon
ThCO2: Theoretical amount of formed Carbon dioxide
TIC: total inorganic carbon
TOC: total organic carbon
TS: test substance assay
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance is not readily biodegradable according to OECD criteria. it is poorly biodegradable.
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