Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 812-497-9 | CAS number: 1893414-79-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Limit test instead of full test - due to an error of the testing laboratory the effect concentration observed was below the lowest the effect concentration of other tested taxa - this finding was not reported to the sponsor. Test will be repeated.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- Limit test instead of full test - due to an error of the testing laboratory the effect concentration observed was below the lowest the effect concentration of other tested taxa - this finding was not reported to the sponsor. Test will be repeated.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: Phosphoric acid, C14-15 branched and linear alkyl esters, potassium salts
CAS No.: 1893414-79-3
Physical state: white solid at 20 °C
Batch No.: PU61810016
Re-certification date of batch: 09 March 2018
Purity: 100 % (UVCB, lyophilized solid, water content 0.85 % (w/w))
Stability: stable under test conditions
Storage condition of test material: Room temperature, protected from light - Analytical monitoring:
- yes
- Details on sampling:
- Analytical data are required by the guidelines for verification of test item concentrations as well as the stability of the test item over the entire test period. Analytical samples were taken in 24 h intervals from fresh and aged test solutions. For each sampling also a retain sample was taken. 500 µL samples were taken and stabilized with 500 µL methanol (MeOH). All samples were stored deep frozen until they were transferred to the analytical laboratory.
- Vehicle:
- no
- Details on test solutions:
- Based on the lowest EC50 value of existing and reliable algae or acute invertebrate toxicity data, the following nominal test item concentration was tested in the main test: 100, 45.5, 20.7, 9.39 und 4.27 mg/L and control (see description of deviations caused by testing laboratory above). The necessary amount of test item for preparing the stock solution S1 was weighed on a weighing scoop and transferred to a volumetric flask. Test medium was added up to the bench mark and the stock solution was homogenised by shaking. Afterwards the solution was slightly turbid milky and white flakes appeared. The stock solution S1 was then transferred to the test vessel. The preparation procedure was repeated every 24 hours.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The species used for the test was Danio rerio Hamilton (Cypriniformes: Cyprinidae). This species is a recommended sensitive indicator species for testing the side effects of chemicals. Fish from the own stock breeding of the test facility were used. The parent animals were originally purchased from the Karlsruhe Institute of Technology (KIT, Karlsruhe, Germany) in a healthy condition. According to the supplier they had been bred from wild strain (WIK) zebrafish before. Since they have been purchased, the zebrafish were continuously reared in the laboratory of the testing facility. Only fish in good health and free from any apparent malformation were used. The last feeding was performed one day prior test start.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- The holding water was composed of reconstituted test water consisting of analytical grade salts dissolved in purified water. The water parameters were controlled 3 times a week. The total hardness was between 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH.
- Test temperature:
- The water temperature was 21 to 25 °C.
- pH:
- The pH-value of the aerated water was between 6.0 - 8.5.
- Dissolved oxygen:
- For at least seven days prior to testing the dissolved oxygen was above 80 % saturation.
- Salinity:
- The holding water was composed of reconstituted test water consisting of analytical grade salts dissolved in purified water. The ratio of Ca:Mg and Na:K was 4:1 and 10:1, respectively, based on molarity. The water parameters were controlled 3 times a week.
- Nominal and measured concentrations:
- The content of the substance in test solutions was analytically determined to confirm the correct application of the test item. The measured content of the test item in fresh test solutions was between 16.5 % and 39.7 % of nominal. The measured content of the test item in aged test solutions was between 6.79 % and 45.3 % of nominal. Since the measured concentrations of the test item in all test item solutions were below 80 % of nominal concentrations, the biological endpoints were evaluated using the mean measured test item concentration. Using mean measured concentrations for study evaluation is an acceptable according to the Guideline OECD 203, when the start values are outside the range of 80 % and 120 %.
- Details on test conditions:
- Biological Assessment
Fish were observed at 0, 4, 24, 48, 72 and 96 hours after test start. Fish were considered dead if there was no visible movement (e.g. gill movement), and if touching of the caudal peduncle produced no reaction. Records were made on visible abnormalities as: loss of equilibrium, swimming behaviour, respiratory function, pigmentation and all other observed events. Dead fish were removed if observed and mortality, length and weight were recorded. At termination of the test, all remaining fish were euthanized and all fish were weighed and measured.
Physico-Chemical Assessments
Measurements of temperature, pH-value and oxygen saturation were performed in 24 hour intervals in fresh and aged test solutions. Water hardness of the untreated control was determined at the beginning of the test. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- not specified
- Reported statistics and error estimates:
- not determined
- Validity criteria fulfilled:
- not applicable
- Remarks:
- see description below
- Conclusions:
- According to the results of the OECD 203 limit test, the LC50 (96 h) of the test item was determined to be > 23.4 mg/L based on nominal test item concentrations and > 6.32 mg/L based on mean measured concentrations. The corresponding NOEC (mortality) (96 h) was 23.4 mg/L based on nominal test item concentrations and 6.32 mg/l based on mean measured concentrations.
- Executive summary:
According to the results of the OECD 203 limit test, the LC50 (96 h) of the test item was determined to be > 23.4 mg/L based on nominal test item concentrations and > 6.32 mg/L based on mean measured concentrations. The corresponding NOEC (mortality) (96 h) was 23.4 mg/L based on nominal test item concentrations and 6.32 mg/l based on mean measured concentrations. No sublethal effects were observed in the control and at the threshold concentration of 23.4 mg/L (test item nominal) and 6.32 mg/L (test item mean measured) after 96 h. Since the measured concentration, 6.32 mg/L is below the measured concentration of the most sensitive organism Daphnia magna, 7.96 mg/L (Kümmich, F. 2017), the conclusion that fish is not the most sensitive organism is not possible.
Reference
Description of key information
According to the results of the OECD 203 limit test, the LC50 (96 h) of the test item was determined to be > 23.4 mg/L based on nominal test item concentrations and > 6.32 mg/L based on mean measured concentrations. The corresponding NOEC (mortality) (96 h) was 23.4 mg/L based on nominal test item concentrations and 6.32 mg/l based on mean measured concentrations.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.