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EC number: 257-775-5 | CAS number: 52238-69-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Remarks:
- source study on the analogous substance
- Adequacy of study:
- key study
- Study period:
- From November 18 to December 07, 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- adopted February 24, 1987
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Similar Substance 01
- IUPAC Name:
- Similar Substance 01
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Wiga, Sulzfeld, West-Germany.
- Age at study initiation: 9 weeks.
- Weight at study initiation: 308 - 377 g males; 229 - 234 g females.
- Housing: individually housed in polycarbonate cages containing purified sawdust as bedding material (Woody Clean supplied by Broekman Institute, Someren, The Netherlands).
- Diet: standard pelleted laboratory animal diet (RMH-B from Hope Farms, Woerden, The Netherlands), ad libitum.
- Water: tap-water, diluted with decalcified water, ad libitum.
- Acclimation period: 5 days under laboratory conditions.
ENVIRONMENTAL CONDITIONS
- Temperature: 21 ± 3 °C
- Relative dumidity: 30 - 70 %
- Air changes: 7.5-15 air changes per hour.
- Photoperiod: 12 hours artificial fluorescent light/12 hours dark per day.
Administration / exposure
- Vehicle:
- water
- Remarks:
- prepared by reverse osmosis
- Details on dermal exposure:
- FORMULATION
The formulations were prepared immediately prior to dosing. The test article was weighed into a glass flask on an analytical balance and the vehicle (w/w) was added.
Homogeneity of the test article in vehicle was obtained by a homogeniser.
TREATMENT
- Shaving: one day before treatment (day -1) an area of approximately 5 x 7 cm on the back of the animal was clipped.
- Application: approximately 25 cm^2 (5 x 5 cm) for males and 18 cm2 (3.5 x 5 cm) for females was applied using a gauze patch fixed, successively, to aluminium foil and flexible bandage (Cohan, 3M, St. Paul, U.S.A.), with drops of vaseline.
- Dose volume: 10 ml/kg bw - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 5 males and 5 females
- Details on study design:
- - Duration of observation period following administration: 14 days.
- Frequency of observations: at periodic intervals on the day dosing (day 1) and twice daily thereafter for at least 14 days. All signs of reaction to treatment were recorded with particular attention paid to changes in the skin, fur, eyes and mucous membranes, as well as to behaviour pattern, tremors, convuision, salivations, diarrhoea, lethargy, sleep and coma. Changes of the treated skin were described immediately after bandage removal (day 2), on day 5, 8 and 15.
- Frequency of weighing: test days 1 (pre-administration), 8 and 15.
- Necropsy of survivors performed: all animals were necropsied and descriptions of all macroscopic abnormalities were recorded. All animals surviving to the end of the observation period were sacrified by carbon dioxide asphyxiation and subjected to necropsy.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- no indication of skin irritation up to the relevant limit dose level
- Mortality:
- No mortality occurred during the study period.
- Clinical signs:
- There were no treatment related signs of toxicity over the 15 day observation period.
The treated skin surface of animals showed no irritation. During the study period all animals showed orange discolouration of the treated skin, due to staining by the test substance. - Body weight:
- With the exception of animal 9, all animals showed body weight gain during the study period.
- Gross pathology:
- Macroscopic examination of all animals at the end of the study showed in one female red discolouration and thickened wall of the forestomach accompanied by blister-like areas.
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified, according to the CLP Regulation (EC) No 1272/2008
- Conclusions:
- LD50 (male and female) > 2000 mg/kg bw
- Executive summary:
The substance was administered to rats of both sexes by dermal application at 2000 mg/kg body weight for 24 hours. No mortality occurred during the study period.
There were no treatment related signs of toxicity over the 15 day observation period. With the exception of animal 9, all animals showed body weight gain during the study period. The treated skin surface of animals showed no irritation. During the study period all animals showed orange discolouration of the treated skin, due to staining by the test substance. Macroscopic examination of all animals at the end of the study showed in one female red discolouration and thickened wall of the forestomach accompanied by blister-like areas.
The dermal LD50 value in rats of both sexes was estimated to exceed 2000 mg/kg body weight.
Conclusion
LD50 (male and female) > 2000 mg/kg bw
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