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Diss Factsheets
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EC number: 422-030-7 | CAS number: 91538-82-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Apr 11-13, 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- none
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Remarks:
- The water solubility was determined in the corresponding study (reference 4.8-1).
- Vehicle:
- no
- Details on test solutions:
- Reconstituted water according to ELENDT (1990):
Macro nutrients (mg/L):
CaCI2 x 7 H20 293.80
MgSO4 x 7 H20 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3 x 9H20 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Trace elements (mg/L):
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Macro nutrients (mg/L):
Na2EDTA x 2H20 2.50
Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75
After preparation, the reconstituded water was aerated for 24 hours.
- Hardness: about 14° dH (about 250 mg/L CaCO3)
- pH: 7.9 ± 0.3, after an aeration for 24 hours.
References:
ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990 - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Strain: Straus
- Source: in house
- Age at study initiation (mean and range, SD): not older than 24 hours
- Feeding during test: yes
- Food type: suspension of fresh water algae
- Amount: sufficient
- Frequency: once a week - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 14 ° dH (about 250 mg/L CaCO3)
- Test temperature:
- 21 °C
- pH:
- 7.9- 8.0
- Dissolved oxygen:
- 95-98 %
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- -- Preparation
The test medium (reconstituted water and test material) was freshly prepared. Therefore, the test material was solved in the vehicle, reconstituted water
-- Administration
At the start of the experimental phase, 5 Daphnia magna were placed into 10 ml of reconstituted water (control group) or test medium (test material group). The Daphnia magna were not fed, and the control medium and test medium were not aerated during the test.
The test vessels were labeled to assure unique identification.
-- Dose levels
A single test material concentration of 0.1 g/L was tested in an open static system.
-- Observation schedule
The mobility was determined by visual control and recorded after 24 and 48 hours.
-- pH and oxygen concentration
The pH-values and dissolved oxygen concentration (O2) were measured in the control and all test material concentrations at the beginning and at the end of the experimental part.
-- Temperature
During the experimental part, the temperature was registered in a control vessel with an electronic thermometer containing a maximum and minimum memory display.
-- Archive statement
All raw data, specimens and the final report are stored in the archives of the test facility. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: >0.0114 mg/L (limit of water solubility)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: >0.0114 mg/L (limit of water solubility)
- Details on results:
- A nominal test concentration of 100 mg/L revealed no aquatic toxicity in this test system. The solubility of the test item was determined to be >0.0114 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg per L of test item were not affected. For the test material the following EC50 values for Daphnia magna were determined: 24 h EC50 > 0,0114 mg/L (nominal > 100 mg/L) 48 h EC50 > 0,0114 mg/L (nominal > 100 mg/L)
- Executive summary:
Purpose
The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments.
Study design
For this purpose, juvenile Daphnia magna were exposed to an aqueous test material preparation over 48 hours, under defined conditions. The GLP study was performed as a limit test according to OECD TG 202.
Results
The follwing results have been obtained:
Nominal concentration Immobilization immobilized / exposed
[g/L] 24 hours 48 hours 0.0 0/20 0/20 0.1 0/20 0/20 Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg per L of test item were not affected.
For the test material the following EC50 values for Daphnia magna were determined: 24 h EC50 > 0,0114 mg/L (nominal > 100 mg/L)
48 h EC50 > 0,0114 mg/L (nominal > 100 mg/L)
NOEC > 0.1 g/L (nominal)
LOEC > 0.1 g/L (nominal)
Conclusions
Under the given experimental conditions, the test material showed no aquatic toxicity. The 48 hours EC50 to Daphnia magna is > 100 mg/L.
Reference
The concentration of the test item and the immobilization data are shown in the following table:
|
Immobilized/Exposed Daphnia Magna |
|
Nominal concentration [mg/L] |
24 h |
48 h |
0 |
0/20 |
0/20 |
100 |
0/20 |
0/20 |
A dose group with nominal 100 mg/L of the test item (maximal water solubility 0.0114 mg/L) revealed no aquatic toxicity in this test system. No remarkable observations were made concerning the appearance of the solution of the test material preparation. The test medium was a clear preparation and stayed unchanged throughout the study.
Description of key information
A dose group with nominal 100 mg/L of the test item (maximal water solubility 0.0114 mg/L) revealed no aquatic toxicity in this test system.
Key value for chemical safety assessment
Additional information
A study was conducted according to OECD Guideline 202 in accordance with good laboratory practice. 20 Daphnias (Strauss) were exposed to the test item in different concentrations. Control experiments were run witth water without andy further additives. After 24 and 48 h the immobilized animals were determined. The test item had did not show any effect at a nominal concentration if 100 mg/L with a maximum water solubility of 0.0114 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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