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EC number: 250-792-9 | CAS number: 31765-95-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- published in O.J.L 142, 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: Saturn Grey LCGCAS: 31765-95-4Batch No.: 8010/2008Appearance: black powderStorage: dark dry room, closed container, at laboratory temperatureExpiration date: February 2018
- Analytical monitoring:
- yes
- Details on sampling:
- The analytical determination of the test substance concentrations were performed at the beginning of the test, before renewal of the test solutions, after renewal of the test solutions and at the end of the test. The samples for analysis were taken from the highest (100 mg/l) and the lowest test concentration (1 mg/l). All samples for analysis were delivered in transport box to analytical laboratory immediately after preparation and after each test period (0-24, 24-48, 48-72 and 72-96 hours). An analysis of samples was started immediately after delivery. All samples were stored at laboratory temperature.
- Vehicle:
- no
- Details on test solutions:
- DILUTION WATERStandard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D in 1000 mL of deionized water.A. 117.6 g CaCl2·2H20 in 1L deionized waterB. 49.3 g MgSO4·7H2O in 1L deionized waterC. 25.9 g NaHCO3 in 1L deionized waterD. 2.3 g KCl in 1L deionized waterThe dilution water should have a pH 7.8 ± 0.2, values measured in this study were 7.8 – 8.0 in the preliminary semi-static test. The content of sum Ca + Mg ions should have the value of 2.50 ± 0.10 mmol/l, values measured in this study were 2.52 – 2.54 mmol/l in the preliminary semi-static test. Measured values meet the requirements.PREPARATION OF SOLUTIONS OF THE TEST SUBSTANCEThe stock solution of the test substance was prepared in the dilution water. 200 mg of the test substance was weighed into 2000 ml of the dilution water for the preliminary semi-static test. The concentrations of solutions used in the preliminary semi-static test were obtained by dilution of the stock solution with dilution water.
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- Species: Poecilia reticulata Supplier: certified supplier EMPLA s.r.o., Hradec Králové (Czech Republic) Date of delivery: 23.3.2016 Length: 2.0 – 3.0 cmAge: 3 - 4 months oldHousing: The fish were kept in all-glass aquaria in aerated drinking water.Diet: “TetraMin” – complete feed for aquarium fish (manufacturer: Tetra GmbH, Germany), once a day.Medical treatment: NoneSensitivity test: Species is periodically monitored by testing with the reference substance - potassium dichromate.Preparation for test: Fish were acclimatized for 7 days in dilution water.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- no
- Test temperature:
- 22 ± 2°C, with a variation of ± 1°C at each individual test
- pH:
- 7.6 - 8.4
- Dissolved oxygen:
- 6.4 - 7.8 mg/l
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- no mortality observed
- Results with reference substance (positive control):
- REFERENCE TESTThe sensitivity of the test species and correctness of test performance is periodically verified in six-month period by testing the reference substance, potassium dichromate.The results of verification test with K2Cr2O7 are the following:96 hour: LC50 = 152 mg/l48 hour: LC50 = 178 mg/l INTERLABORATORY TEST DATAFrom the result of the last interlaboratory test arranged by CSlab spol. s r.o. in 2015 we have obtained data for potassium dichromate 96h – EC50 generated at 12 laboratories. The following reference range of values could be calculated from those data:96 hour: LC50 = 78 – 183 mg/l
- Validity criteria fulfilled:
- yes
- Conclusions:
- The experiment with the concentration of 100 mg/l included in the preliminary semi-static test has been performed using 7 fish, there through the conditions of the limit test have been fulfilled. It was demonstrated that the LC50 is higher than this concentration.
- Executive summary:
The test substance, Direct Black 112, was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method C.1 – Acute toxicity for fish, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed in a range of the test substance nominal concentrations 1 – 100 mg/l. The test was performed as semi-static. The test solutions were renewed after 24 hours. The test substance concentrations were measured at the beginning, after 24, 48, 72 hours (before and after renewal) and at the end of the test at the highest (100 mg/l) and the lowest concentration (1 mg/l). The analytical results from the preliminary semi-static test showed, that the test substance was stable in dilution water at conditions of the test.
Based on no toxicity of the test substance found in the preliminary semi-static test, the study was terminated.The experiment with the concentration of 100 mg/l included in the preliminary semi-static test has been performed using 7 fish, therethrough the conditions of the limit test have been fulfilled. It was demonstrated that the LC50is higher than this concentration.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.
Test results:
24 hour – LC50 > 100 mg/l
48 hour – LC50 > 100 mg/l
72 hour – LC50 > 100 mg/l
96 hour – LC50 > 100 mg/l
24 hour – LC0 = 100 mg/l
48 hour – LC0 = 100 mg/l
72 hour – LC0 = 100 mg/l
96 hour – LC0 = 100 mg/l
24 hour – LC100 > 100 mg/l
48 hour – LC100 > 100 mg/l
72 hour – LC100 > 100 mg/l
96 hour – LC100 > 100 mg/l
Reference
Description of key information
The experiment with the concentration of 100 mg/l included in the preliminary semi-static test has been performed using 7 fish, there through the conditions of the limit test have been fulfilled. It was demonstrated that the LC50 is higher than this concentration.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
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