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EC number: 264-796-3 | CAS number: 64346-30-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of read across chemicals
- Justification for type of information:
- Data from handbook or collection of data
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- WoE was prepared to determine the effect of test chemical on the mobility of aquatic invertebrates Daphnai magna.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- WoE 2, 3: The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution.
WoE 4:
The stock solution (10 mg/L) was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24 and 48 hrs
- Hardness:
- 145.5 mg of CaCO3
- Test temperature:
- 20±2 ° C
- pH:
- WoE 2: 7.1
WoE 4: Sample at concentration 0.32 mg/L: pH= 8.0 changed to pH= 7.9 during the test
Control: pH= 8.0 changed to pH= 7.9 during the test - Dissolved oxygen:
- higher than 8 mg/L at the end of test
- Nominal and measured concentrations:
- WoE 2, 3: 0.125mg/L, 0.25mg/L, 0.5mg/L,1mg/L, 2mg/L nominal concentrations were used.
WoE 4: 0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
WoE 4:
Details on test conditions
TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: WoE - 2
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.125 - < 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: WoE - 3
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.116 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: WoE - 4
- Validity criteria fulfilled:
- yes
- Conclusions:
- 2: Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on test Daphnia was determine to be 0.5 mg/L.
3: Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on Daphnia magna was determine to be <0.25 mg/L but >0.125 mg/L.
4: The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 0.016 mg/L on the basis of mobility inhibition effects in a 48 hour study.
Under the test condition by observing the effects on the mobility of Daphnia magna, it was consider that the chemical was toxic and can be consider to be classified in aquatic acute 1/chronic 1 as per the CLP classification criteria. - Executive summary:
Data available for the test chemical has been reviewed to determine the toxicity of test chemical on the mobility of aquatic invertebrates. The studies are as mentioned below:
Principle of this study was to observe the effect of test chemical on the mobility pattern of aquatic invertebrates. Test conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism. The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution. 10 Daphnids was added per concentration. Test conducted under the static system for 48 hours. The effect of test chemical was calculated on the basis of immobility observations of Daphnia magna. Observations including (immobility, pH, Temperature, dissolved oxygen content) were recorded in the interval of 24 and 48 hours. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on Daphnia was determine to be 0.5 mg/L. Based on the EC50 value, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.
Above study was further supported by the study from experimental source. Principle of this study was to observe the effect of test chemical on the mobility pattern of aquatic invertebrates. Test conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism. The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution. 10 Daphnids was added per concentration. Test conducted under the static system for 48 hours. The effect of test chemical was calculated on the basis of immobility observations of Daphnia magna. Observations including (immobility, pH, Temperature, dissolved oxygen content) were recorded in the interval of 24 and 48 hours. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on test daphnia was determine to be <0.25 mg/L but >0.125 mg/L. Thus based on the EC50 value, it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.
Similar study for determination of the inhibition of the mobility of Daphnia magna was carried out with the test substance conducted according to OECD Guideline 202. Test performed under the static system. The test substance was tested at the concentrations 0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L. Effects on immobilisation were observed for 48 hours. EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 0.016 mg/L on the basis of mobility inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrate Daphnia magna and can be classified as aquatic acute category 1/ chronic 1 as per the CLP criteria.
Under the test condition by observing the effects on the mobility of Daphnia magna, it was consider that the chemical was toxic and can be consider to be classified in aquatic acute 1/chronic 1 as per the CLP classification criteria.
Reference
Description of key information
2: Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on test Daphnia was determine to be 0.5 mg/L.
3: Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on Daphnia magna was determine to be <0.25 mg/L but >0.125 mg/L.
4: The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 0.016 mg/L on the basis of mobility inhibition effects in a 48 hour study.
Under the test condition by observing the effects on the mobility of Daphnia magna, it was consider that the chemical was toxic and can be consider to be classified in aquatic acute 1/chronic 1 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.5 mg/L
Additional information
Data available for the test chemical and structurally and functionally similar read across chemicals extracted using mechanistic approach has been reviewed to determine the toxicity of test chemical on the mobility of aquatic invertebrates. The studies are as mentioned below:
Principle of this study was to observe the effect of test chemical on the mobility pattern of aquatic invertebrates. Test conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism. The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution. 10 Daphnids was added per concentration. Test conducted under the static system for 48 hours. The effect of test chemical was calculated on the basis of immobility observations of Daphnia magna. Observations including (immobility, pH, Temperature, dissolved oxygen content) were recorded in the interval of 24 and 48 hours. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on Daphnia was determine to be 0.5 mg/L. Based on the EC50 value, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.
Above study was further supported by the study from experimental source. Principle of this study was to observe the effect of test chemical on the mobility pattern of aquatic invertebrates. Test conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism. The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution. 10 Daphnids was added per concentration. Test conducted under the static system for 48 hours. The effect of test chemical was calculated on the basis of immobility observations of Daphnia magna. Observations including (immobility, pH, Temperature, dissolved oxygen content) were recorded in the interval of 24 and 48 hours. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on test daphnia was determine to be <0.25 mg/L but >0.125 mg/L. Thus based on the EC50 value, it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.
Similar study for determination of the inhibition of the mobility of Daphnia magna was carried out with the test substance conducted according to OECD Guideline 202. Test performed under the static system. The test substance was tested at the concentrations 0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L. Effects on immobilisation were observed for 48 hours. EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 0.016 mg/L on the basis of mobility inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrate Daphnia magna and can be classified as aquatic acute category 1/ chronic 1 as per the CLP criteria.
Under the test condition by observing the effects on the mobility of Daphnia magna, it was consider that the chemical was toxic and can be consider to be classified in aquatic acute 1/chronic 1 as per the CLP classification criteria.
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