Ethyl undec-10-enoate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 - 27 Sept 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 202 of 1984 and additional suggestions for OECD 202 part 2 of 1993

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isopropyl myristate

EC Number:

203-751-4

EC Name:

Isopropyl myristate

Cas Number:

110-27-0

Molecular formula:

C17H34O2

IUPAC Name:

isopropyl myristate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Two stock solutions with nominal concentrations of 1 and 100 mg/L M4 medium; 16 h to 7 d magnetic stirring; suspensions were filtered via glas fibre filter (pre-treated with NaOH)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture, originally obtained from BGA Berlin, May 1994
- Feeding during test: yes
- Food type: Scenedesmus subspicatus
- Amount: 0-7 d old animals: 2.5*10E6 cells/daphnid/d; >7d old animals: 5.0*10E6 cells/daphnid/d
- Frequency: daily

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Post exposure observation period:

not relevant

Test conditions

Test temperature:

21.6 - 23.2°C

pH:

7.7 - 8.2

Dissolved oxygen:

84 - 100% saturation (90-100% in control; 84-97% in 1 mg/L; 86-98% in 100 mg/L)

Nominal and measured concentrations:

nominal: 0, 1 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL Erlenmeyer flask
- Type: closed with watchglass
- Material, size, headspace, fill volume: 50 mL test solution
- Renewal rate of test solution (frequency/flow rate): every 2 to 3 days
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: 300 Lux

EFFECT PARAMETERS MEASURED: number of offspring per daphnid

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOELR

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: filtered test solution

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: filtered test solution

Basis for effect:

reproduction

Details on results:

- Mortality of parent animals: 0%
- No. of offspring produced per day per female: 4.3 daphnids/day in the control; 4.1 daphnids/day in 1 mg/L TS and 4.0 daphnids/day in 100 mg/L test substance
- Time to first brood release or time to hatch: 7 days after test begin in the control

Any other information on results incl. tables

Table 1: Reproduction and mortality after 21 days:

Concentration (mg/L)	Average reproduction (offspring/daphnid/day)	Mortality (%)
0	4.3	0
1	4.1	0
100	4.0	0