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EC number: 605-718-3 | CAS number: 174350-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jun 06 - Dec 02, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- July, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was insufficiently soluble to permit addition via aqueous solution. Individually weighed quantities of test substance were added directly to the appropriate test vessels.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The activated sludge inoculum was collected from the sludge return line at Pool or Burley Menston sewage treatment works (West Yorkshire, UK), which has a predominantly domestic catchment. The point of collection was to ensure that the activated sludge sample was relatively free of exogenous material.
Preparation and Maintenance
The sludge was transported to the test facility in a closed container with an adequate headspace to prevent the sample becoming anoxic. On arrival at the test facility, the sludge was aerated with a compressed air supply delivered through a suitable distribution device.
The suspended solids concentration was determined gravimetrically following homogenisation and adjusted using dechlorinated tap water to 3 g/L (± 0.3 g/L). The sludge was maintained at 20 ± 2 °C with aeration. During storage it was maintained at 20 ± 2 °C and fed with synthetic sewage concentrate at a rate of 50 mL/L.
An activated sludge sample that provided the test inoculum for the range-finder test was collected on 7 June 2016, the activated sludge sample that provided the test inoculum for the definitive limit test was collected on 20 July 2016. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.01 - 7.25
- Dissolved oxygen:
- saturated
- Nominal and measured concentrations:
- Range Finder: 1, 10, 100 and 1000 mg/L
Definitive Limit: 1, 10, 100 and 1000 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: ca 250 mL glass conical flask
- Type (delete if not applicable): open
- Aeration:compressed air supply
- No. of vessels per concentration (replicates): range finder: 2, definitive: 3
- No. of vessels per control (replicates): range finder: 1, definitive: 1
- No. of vessels per vehicle control (replicates): range finder: 4, definitive: 1
- Sludge concentration (weight of dry solids per volume): 3 g/L
- Weight of dry solids per volume of reaction mixture per unit of time:
- Nutrients provided for bacteria: no
- Nitrification inhibitor used (delete if not applicable): N-allylthiourea
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
OTHER TEST CONDITIONS
- Adjustment of pH: yes
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Justification for using fewer concentrations than requested by guideline: n.a.
- Range finding study yes
- Test concentrations: 1, 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: range finding results - Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Results with reference substance (positive control):
- 3,5-DCP:
Total Respiration: 10.1 mg/L
Nitrification Respiration: 3.3 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- There was no statistically significant inhibition of respiration observed during the test. The EC50 for the test item was greater than 1000 mg/L under these test conditions. The NOEC value was determined to be 1000 mg/L, the highest concentration tested.
- Executive summary:
The aim of this study was to determine the no observable effect concentration (NOEC) and estimate the effect concentration for an x% effect (ECx) for activated sludge exposed to the test substance, where possible. The study was undertaken according to OECD Guideline 209, Activated Sludge, Respiration and Inhibition Test (Carbon and Ammonium Oxidation), adopted July, 2010.
The activated sludge inoculum for the limit test was collected from the sludge return line at Pool Sewage Treatment Works (West Yorkshire, U.K.), which has a predominantly domestic catchment. The point of collection was to ensure that the activated sludge sample was relatively free of exogenous material.
A range-finder test, employing nominal test material concentrations of 1, 10, 100 and 1000 mg/L, was undertaken to determine the appropriate concentration levels for a definitive test. The range-finder test showed no inhibition of total, or heterotrophic respiration, but did show inhibition of nitrification respiration at 100 mg/L and 1000 mg/L.
Based on the results of the range-finding test, a definitive limit test was conducted at concentrations of 1, 10, 100 and 1000 mg/L. Blank controls were also included.
The results of the definitive test showed no inhibition of total or heterotrophic respiration at all concentrations up to, and including, 1000 mg/L. A limited effect was observed on nitrification respiration at 100 mg/L (24 % inhibition) but this was not statistically significant.
As only limited inhibition was observed, determination of EC50 was not possible and is therefore reported to be greater than 1000 mg/L (the highest concentration tested under these conditions). The NOEC for the test item is reported to be 1000 mg/L.
Reference
Description of key information
There was no statistically significant inhibition of respiration observed during the test. The EC50 for the test item was greater than 1000 mg/L under these test conditions. The NOEC value was determined to be 1000 mg/L, the highest concentration tested.
Key value for chemical safety assessment
Additional information
The aim of this study was to determine the no observable effect concentration (NOEC) and estimate the effect concentration for an x% effect (ECx) for activated sludge exposed to the test substance, where possible. The study was undertaken according to OECD Guideline 209, Activated Sludge, Respiration and Inhibition Test (Carbon and Ammonium Oxidation), adopted July, 2010.
The activated sludge inoculum for the limit test was collected from the sludge return line at Pool Sewage Treatment Works (West Yorkshire, U.K.), which has a predominantly domestic catchment. The point of collection was to ensure that the activated sludge sample was relatively free of exogenous material.
A range-finder test, employing nominal test material concentrations of 1, 10, 100 and 1000 mg/L, was undertaken to determine the appropriate concentration levels for a definitive test. The range-finder test showed no inhibition of total, or heterotrophic respiration, but did show inhibition of nitrification respiration at 100 mg/L and 1000 mg/L.
Based on the results of the range-finding test, a definitive limit test was conducted at concentrations of 1, 10, 100 and 1000 mg/L. Blank controls were also included.
The results of the definitive test showed no inhibition of total or heterotrophic respiration at all concentrations up to, and including, 1000 mg/L. A limited effect was observed on nitrification respiration at 100 mg/L (24 % inhibition) but this was not statistically significant.
As only limited inhibition was observed, determination of EC50 was not possible and is therefore reported to be greater than 1000 mg/L (the highest concentration tested under these conditions). The NOEC for the test item is reported to be 1000 mg/L.
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