Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 251-528-5 | CAS number: 33454-82-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 24 sept 2012 to 23 January 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Trifluoromethanesulphonic acid
- EC Number:
- 216-087-5
- EC Name:
- Trifluoromethanesulphonic acid
- Cas Number:
- 1493-13-6
- Molecular formula:
- CHF3O3S
- IUPAC Name:
- trifluoromethanesulfonic acid
- Test material form:
- liquid
- Details on test material:
- See confidential details on test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At test initiation (0 hour) and test termination (48 hours), a single sample was removed from each test concentration and the control and analyzed for trifluoromethanesulfonic acid. The sample analyzed at 0 hour was removed from the test solution prior to division into the replicate vessels. Samples analyzed at test termination were composited prior to analysis. Each sample was collected from the approximate midpoint of the vessels and analyzed for trifluoromethanesulfonic acid.
Three quality control (QC) samples were also prepared in dilution water at each sampling interval at nominal concentrations which approximated the test concentration range and remained with the exposure solution samples throughout the analytical process. Analysis of the QC samples was used to judge the precision and quality control maintained during the analytical process.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A 100 mg/L stock solution was prepared prior to test initiation by adding 0.2500 g of the test substance to 2.5 L of dilution water. The pH of the stock solution was adjusted from 6.7 to 8.1 using 1.56 mL of 1.0 N sodium hydroxide. The resultant solution was mixed with a stir plate and magnetic stir bar for approximately ten minutes and was observed to be clear and colorless with no visible undissolved test substance. The 100 mg/L primary stock solution was used to prepare exposure solutions of 6.3, 13, 25, 50 and 100 mg/L.
Test solutions were mixed with a glass rod for approximately one minute prior to division into replicate exposure vessels. Following mixing, all test solutions were observed to be clear and colorless with no visible undissolved test substance. Each test solution was divided into four replicate vessels, each containing approximately 200 mL of solution. A set of control solutions was also prepared containing dilution water only and maintained under the same conditions as the treatment level solutions. Exposure vessels were labeled to identify the concentration and study number.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: obtained from laboratory cultures maintained at Smithers Viscient.
- Age at study initiation (mean and range, SD): < 24h old
- Method of breeding: own culture of the lab
- Feeding during test: no
- Feeding during culture: Daphnids were fed unicellular green algae, Ankistrodesmus falcatus (4 x 107 cells/mL) in addition to a suspension of YCT (yeast, cereal leaves and flaked fish food).
ACCLIMATION
- Acclimation period: no, because own culture in the lab in the same conditions as the test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L as CaCO3
- Test temperature:
- 19 to 20ºC
- pH:
- 8.2 to 8.3
- Dissolved oxygen:
- 8.8 to 9.2 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0, 6.3, 13, 25, 50 and 100 mg/L
Measured concentrations: 0, 5.7, 12, 23, 44 and 92 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250-mL glass beakers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Test vessels were 250-mL glass beakers, each containing 200 mL of test solution.
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water used during the definitive test was from the same source as the water in the daphnid cultures
- Total organic carbon:
- Alkalinity: 96 mg/L as CaCO3
- Dissolved oxygen: 10 mg/L
- Conductivity: 620 µs/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: monthly
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:16 hours of light and 8 hours of darkness
- Light intensity: 70 to 81 footcandles (753 to 872 lux)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 0.010, 0.10, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Following 48 hours of exposure, 5, 0, 5, 0 and 0% immobilization was observed among daphnids exposed to the 0.010, 0.10, 1.0, 10 and 100 mg/L treatment levels, respectively. Based on the results of preliminary testing, nominal trifluoromethanesulfonic acid test concentrations of 6.3, 13, 25, 50, and 100 mg/L were selected for the definitive exposure. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Analytical Results:
Measured concentrations were generally consistent throughout the exposure phase and maintained the expected concentration gradient. Mean measured concentrations ranged from 89 to 92% of nominal and defined the treatment levels as 5.7, 12, 23, 44 and 92 mg/L.
Analysis of the six quality control samples resulted in measured concentrations which were consistent with the predetermined recovery range and ranged from 90.9 to 100% of the nominal fortified levels (3.17, 27.2 and 99.8 mg/L). These results established that the appropriate precision and quality control was maintained during the analysis of the exposure solutions.
Biological Results:
Following 48 hours of exposure, no immobilization or adverse effects were observed among daphnids exposed to the any of the treatment levels.
In nominal, the 48-hour EC50 value was empirically estimated to be > 100 mg/L. The No-Observed-Effect Concentration (NOEC) was determined to be 100 mg/L. The highest concentration producing 0% immobilization was 100 mg/L. - Results with reference substance (positive control):
- - Results with reference substance valid? YES.
The 24 hour reference test was conducted from 8 to 9 November 2012 and the results established that the 24-hour EC50 value for Daphnia magna and potassium dichromate was 2.4 mg/L. This result was within the expected range for daphnids and potassium dichromate.
Any other information on results incl. tables
Mean measured concentrations tested, corresponding cumulative percent and number of immobilized organisms, and observations made during the 48‑hour static acute exposure of daphnids (Daphnia magna) to trifluoromethanesulfonic acid.
Nominal Concentration (mg/L) |
Cumulative Percent Immobilization (Number of Immobilized Daphnids)a |
||||||||||
24-Hour |
|
48-Hour |
|||||||||
A |
B |
C |
D |
Mean |
|
A |
B |
C |
D |
Mean |
|
Control |
0 |
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
0 |
(0) |
(0) |
(0) |
(0) |
|
|
(0) |
(0) |
(0) |
(0) |
|
|
6.3 |
0 |
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
0 |
(0) |
(0) |
(0) |
(0) |
|
|
(0) |
(0) |
(0) |
() |
|
|
13 |
0 |
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
0 |
(0) |
(0) |
(0) |
(0) |
|
|
(0) |
(0) |
(0) |
(0) |
|
|
25 |
0 |
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
0 |
(0) |
(0) |
(0) |
(0) |
|
|
(0) |
(0) |
(0) |
(0) |
|
|
50 |
0 |
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
0 |
(0) |
(0) |
(0) |
(0) |
|
|
(0) |
(0) |
(0) |
(0) |
|
|
100 |
0 |
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
0 |
(0) |
(0) |
(0) |
(0) |
|
|
(0) |
(0) |
(0) |
(0) |
|
a The actual number of immobilized daphnids is presented in parentheses.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- - In the control, not more that 10 per cent of the daphnids were immobilised; - The dissolved oxygen concentration at the end of the test was higher than 3 mg/l in control and test vessels.
- Conclusions:
- The 48-hour EC50 value was determined to be > 100 mg/L. The No-Observed-Effect Concentration (NOEC) was determined to be 100 mg/L. Trifluoromethanesulfonic acid is not considered to be harmful to Daphnids.
- Executive summary:
The purpose of the study was to estimate the acute toxicity (EC50) of trifluoromethanesulfonic acid to daphnids (Daphnia magna) under static test conditions. This study was conducted according to OECD Guideline for Testing of Chemicals 202, the EU Method C.2 and the GLP.
The nominal concentrations tested were 6.3, 13, 25, 50, and 100 mg/L.
No immobilised daphnids were observed during the study, therefore the 48-hour EC50 value was > 100 mg/L and the No-Observed-Effect Concentration (NOEC) was determined to be 100 mg/L.
In these test conditions, Trifluoromethanesulfonic acid is not harmful to daphnids.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.