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EC number: 500-734-6 | CAS number: 162492-01-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- toxicity control of ready biodegradability test
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 May - 17 June 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesanstalt für Umwelt, Messungen und Naturschutz Baden-Württemberg, Germany (29.06.2016)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 52.4 mg test item and 5.15 mL of the reference stock (10 g/L sodium benzoate) were added to the test vessel, corresponding to a concentration of 40 mg/L total organic carbon. Mixing was performed with a magnetic stirrer with 2 cm stir bars. The final test volume in the test vessel was 1500 mL, consisting of 1490.9 mL mineral medium and 9.1 mL activated sludge (30 mg/L dry solids). The test vessels (containing mineral medium and activated sludge) were prepared 1 d before test start, sealed, aerated with CO2-free air and mixed with a magnetic stirrer overnight. The following day, the absorber bottles were filled with 0.2 M NaOH (CO2 trap) and the test item and reference compound were introduced and the test started.
- Controls: Only inoculum (blank) - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Municipal wastewater treatment plant Breisgauer Bucht, Germany (sampled 17 May 2016)
- Preparation of inoculum for exposure: The activated sludge was washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge.
- Initial biomass concentration: 4.95 g/L mean dry solid content of activated sludge, determined after drying at 105 °C - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Test temperature:
- 22.3 - 23.6 °C
- Nominal and measured concentrations:
- 34.9 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Gas wash bottles (2000 mL); Fill volume: 1500 mL
- Aeration: With CO2-free air overnight
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Sludge concentration (weight of dry solids per volume): 30 mg/L dry solids
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Mineral medium
OTHER TEST CONDITIONS
- Light intensity: diffuse light
- Details on termination of incubation: The CO2 produced in the reactors was absorbed in two 250 mL gas wash bottles in series each filled with 200 mL 0.2 M NaOH. Sampling was performed through the lateral connecting pieces through the butlyl rubber septum using 5 mL PE syringes.
EFFECT PARAMETERS MEASURED:
Degradation was followed by determining the amount of CO2 produced and absorbed to sodium hydroxide via IC-measurement (IC = inorganic carbon) on Days 0, 4, 8, 11, 14, 21, 28, and 29.
TEST CONCENTRATIONS
- Justification for using fewer concentrations than requested by guideline: Toxicity control of ready biodegradability test (Modified Sturm Test) - Reference substance (positive control):
- yes
- Remarks:
- sodium benzoate
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 34.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: degradation
- Remarks on result:
- other:
- Remarks:
- 46.5% degradation; 52.4 mg test item in 1500 mL test medium
- Validity criteria fulfilled:
- yes
Reference
The degradation in the toxicity control after 14 d was 46.5% and thus above the criterion for inhibition effects to the inoculum. The test item had no toxic effect on the inoculum according to the validity criteria of OECD 301 on ready biodegradability.
Description of key information
NOEC (14 d) ≥ 34.93 mg/L (nominal, OECD 301)
Key value for chemical safety assessment
Additional information
The microbial toxicity of Hexamethylene diisocyanate, trimers, reaction products with 2-hydroxyethyl acrylate (CAS 162492-01-5) was inferred from the toxicity control of a standard ready biodegradability test conducted according to OECD 301 B and GLP, as recommended by the Guidance document “Chapter R.7b Endpoint specific Guidance on Information Requirements and Chemical Safety Assessment” (ECHA, 2017) in absence of a microbial toxicity test according to OECD 209.
In the CO2 evolution test (Modified Sturm Test), the toxicity control was prepared by inoculating 52.4 mg test item (corresponding to approximately 34.93 mg test item/L) and 5.15 mL of the reference compound stock solution (sodium benzoate), with activated sludge (30 mg/L dry solids) under aerobic conditions in diffuse light for 28 d. Degradation was followed by quantification of the produced CO2 trapped by sodium hydroxide, and expressed as blank corrected percentage of the theoretical CO2 demand (ThCO2).
After 14 d, degradation of the reference compound and test item in the toxicity control was 46.5% and thus above the criterion (< 25% degradation in 14 d) for inhibition effects to the inoculum. Based on this result, the test item had no toxic effect on the inoculum and a NOEC (14 d) of ≥ 34.93 mg/L was derived according to the Guidance document “Chapter R.7b Endpoint specific Guidance on Information Requirements and Chemical Safety Assessment” (ECHA, 2017).
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