6-chlorohexan-1-ol

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Aug 27 2020- Jun 8 2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Test temperature:

35°C

Details on study design: HPLC method:

The HPLC method was performed at neutral pH.
Solutions of reference substances with known log Koc values based on soil adsorption data and the test item were analyzed. The capacity factor (k') of each compound was calculated from its retention time. The log k’ values of the references substances were plotted against the known log Koc values. A linear regression program was used to calculate the calibration curve. Linear regression analysis was performed using the least squares method. The coefficient of correlation (r) was calculated. The log Koc value for the test item was calculated by substituting its mean log k’ in the calibration curve. The value of log Koc obtained from duplicate measurements was within +- 0.25 log units.

ANALYTICAL METHODS
Instrument: Acquity UPLC system(Waters, Milford, MA, USA)
Detector: Acquity Refractive Index detector (Waters)
Column:Acquity UPLC HSS Cyano, 100 mm x 2.1 mm i.d., dp = 1.8 μm (Waters)
Column temperature: 35°C +- 1°C
Injection volume: 5 μL
Mobile phase: 55/45 (v/v) methanol/water
Flow: 0.4 mL/min
RI detection

Temperature: 35°C
Sensitivity:4
Time constant: 1 seconds
Polarity: positive

Key result

Sample No.:

#2

Type:

log Koc

Value:

3.06

Temp.:

35 °C

Key result

Sample No.:

#1

Type:

log Koc

Value:

1.59

Temp.:

35 °C

Validity criteria fulfilled:

yes

Conclusions:

The HPLC method using soil-adsorption-reference data was applied for the determination of
the adsorption coefficient (Koc) of the test substance
The Koc and log Koc values of the test item at neutral pH were:
1. Test item peak 1 (major): Koc: 3.9 * 101; log Koc: 1.59; Peak area: 99.4 %
2. Test item peak 2: Koc: 1.1 * 103; log Koc: 3.06; Peak area: 0.6 %

Description of key information

The HPLC method using soil-adsorption-reference data was applied for the determination of
the adsorption coefficient (Koc) of the test substance
The Koc and log Koc values of the test item at neutral pH were:
1. Test item peak 1 (major): Koc: 3.9 * 101; log Koc: 1.59; Peak area: 99.4 %
2. Test item peak 2: Koc: 1.1 * 103; log Koc: 3.06; Peak area: 0.6 %

Key value for chemical safety assessment

Koc at 20 °C:

3.9