2-cyano-N-methylacetamide

Endpoint summary

Description of key information

The acute oral toxicity of 2 -cyano-N-methylacetamide was investigated in male and female rats according to a procedure similar to OECD testing guideline 423. Doses of 215 mg/kg bw, 316 mg/kg bw, 681 mg/kg bw, 1000 mg/kg bw, 1470 mg/kg bw and 2150 mg/kw bw were applied to 5 animals per sex per dose. The main symptoms of intoxication were dyspnea, apathy, abnormal body positions, staggering, tonic cramps, ruffled haircoat, skin redness, hypohydration and a general bad condition of the animals, starting at the lowese dose level partially already 15 min after application. The substance produced a variety of systemic effects. All male animals (as well as one female rat) died in the dose group of 681 mg/kg bw.  Due to this results, the oral toxicity is characterised by a LD50 value of 681 mg/kg bw for both male and female rats together. 

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion

Dose descriptor:

LD50

Value:

681 mg/kg bw

Additional information

Acute oral toxicity:

The acute oral toxicity of 2 -cyano-N-methylacetamide was investigated in male and female rats (BASF 1981a). Doses of 215 mg/kg bw, 316 mg/kg bw, 681 mg/kg bw, 1000 mg/kg bw, 1470 mg/kg bw and 2150 mg/kw bw were applied to 5 animals per sex per dose. The substance was administered by oral gavage. The main symptoms of intoxication were dyspnea, apathy, abnormal body positions, staggering, tonic cramps, ruffled haircoat, skin redness, hypohydration and a general bad condition of the animals, starting at the lowest dose level partially already 15 min after application. All male animals (as well as one female rat) died in the dose group of 681 mg/kg bw. Two femals died within 5 days at a dose of 1000 mg/kg bw. In the 1470 mg/kg bw dose group one female died after 2 days, two further animals died within 5 days. Two animals died within 24 h after a dose of 2150 mg/kg bw, followed by one more animal within the following 5 days. No animal died in the lowest dose group. Dead animals were examined as soon as possible, finding included accumulative hyperemia, bloody ulcerations in the stomach glands region and multiple hämatinised contents in the intestine. No oberservations like this were made in the sacrified animals after 14 days. Due to this results, the oral toxicity is characterised by a LD50 value of 681 mg/kg bw for both male and female rats together.

Acute toxicity after i.p. administration:

The test substance was also administered via the intraperitonial route to male and female mice (BASF 1981b). Doses of 200 mg/kg bw and 700 mg/kw bw were applied to 5 animals per sex per dose in a single application. Main symptoms of intoxication were dyspnea, apathy, abnormal body positions, staggering, tonic cramps, ruffled haircoat, hypohydration and a general bad condition of the animals, starting at the lowest dose level partially already 15 min after application. After 15 min the first clinical signs were observed at both dose levels, including dyspnea, apathy, staggering, convulsions, ruffled haircoat, hypohydration and (a general) bad condition. Additionally, an application of 200 mg/kg resulted in twitching, clonic cramps and spastic gaits of the animals. Petrusions of the eyeballs were observed at the dose level of 700 mg/kg only. After the application of 700 mg/kg all mice (male and female) were dead after 1 day. Three males and one female died 1 day after the application of 200 mg/kg test substance. Neither precipitation of the substance nor adhesions were found in the abdomen of dead and sacrified animals. The LD50 value for both, male and female together was found at 200 mg/kg bw.

Justification for classification or non-classification

Dangerous Substance Directive (67/548/EEC)

The available studies are considered reliable and suitable for classification purposes under 67/548/EEC. As a result the substance is considered to be classified for acute oral toxicity with Xn; R22 under Directive 67/548/EEC, as amended for the 28th time in Directive 2001/59/EC.

Classification, Labelling, and Packaging Regulation (EC) No. 1272/2008

The available experimental test data are reliable and suitable for classification purposes under Regulation 1272/2008. As a result the substance is not considered to be classified for acute oral toxicity with Category 4 (H302) under Regulation (EC) No. 1272/2008, as amended for the second time in Directive (EC 286/2011).