3-bromoquinoline

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data from handbook or collection of data

Data source

Reference

Reference 1

Reference Type:

review article or handbook

Title:

3-Hydroxy-2-naphthalin-carbonsäure [MAK Value Documentation in German language, 2006]

Author:

The MAK Collection for Occupational Health and Safety

Year:

2012

Bibliographic source:

3-Hydroxy-2-naphthalin-carbonsäure [MAK Value Documentation in German language, 2006]. The MAK Collection for Occupational Health and Safety. 1–9. 2012.

Reference 2

Reference Type:

secondary source

Title:

SIDS Report of 3-Hydroxy-2-naphthoic acid

Author:

OECD SIDS

Year:

2004

Bibliographic source:

OECD SIDS; SIDS Initial Assessment Report For SIAM 19 Berlin, Germany; 19-22 October 2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Principles of method if other than guideline:

An ocular irritation test of 3-hydroxynaphthalene-2-carboxylic acid (CAS No: 92-70-6) was conducted on rabbits to assess its irritation potential in accordance with OECD Guide-line 405 "Acute Eye Irritation/Corrosion".

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-2-naphthoic acid

EC Number:

202-180-8

EC Name:

3-hydroxy-2-naphthoic acid

Cas Number:

92-70-6

Molecular formula:

C11H8O3

IUPAC Name:

3-hydroxy-2-naphthoic acid

Test material form:

solid

Details on test material:

- Name of test material (as cited in study report):3-Hydroxy-2-naphthoic acid
- Molecular formula:C11H8O3
- Molecular weight :188.1812 g/mol
- Substance type:Organic
- Physical state:Solid
-Smiles: c12c(cc(c(c1)C(=O)O)O)cccc2
-InChI: 1S/C11H8O3/c12-10-6-8-4-2-1-3-7(8)5-9(10)11(13)14/h1-6,12H,(H,13,14)

Specific details on test material used for the study:

- Name of test material (as cited in study report):3-Hydroxy-2-naphthoic acid
- Molecular formula:C11H8O3
- Molecular weight :188.1812 g/mol
- Substance type:Organic
- Physical state:Solid
-Smiles: c12c(cc(c(c1)C(=O)O)O)cccc2
-InChI: 1S/C11H8O3/c12-10-6-8-4-2-1-3-7(8)5-9(10)11(13)14/h1-6,12H,(H,13,14)

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

No data available

Test system

Vehicle:

other: polyethylene glycol 400

Controls:

not specified

Amount / concentration applied:

100mg (0.5ml)

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

after 1, 24, 48, 72 hours and on day 7 and 14 after application

Number of animals or in vitro replicates:

3

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): eyes were rinsed off after 24 hours
SCORING SYSTEM: Driaze method

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24/72 h

Score:

1.1

Max. score:

4

Reversibility:

not reversible

Remarks on result:

positive indication of irritation

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24/72 h

Score:

0.2

Max. score:

2

Reversibility:

not reversible

Remarks on result:

positive indication of irritation

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 24/72 h

Score:

1.9

Max. score:

3

Reversibility:

not reversible

Remarks on result:

positive indication of irritation

Results 4

Irritation parameter:

other: conjunctival swelling

Basis:

mean

Time point:

other: 24/72 h

Score:

1.3

Max. score:

4

Reversibility:

not reversible

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

1 hour after application, swelling and conjunctival injections as well as secretion (clear, tinted by the test substance) were observed in all animals. At 24, 48 and 72 hours, conjunctivitis and diffuse corneal opacities were found. One animal showed iritis at 24 and 48 hours. 7 days after the application, corneal vascularization was observed in all animals. The chemical may serious eye damage

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

The test material 3-hydroxynaphthalene-2-carboxylic acid (CAS No: 92-70-6) was considered to be irritating to rabbits’ eye with the risk of serious eye damage.

Executive summary:

An ocular irritation test of 3-hydroxynaphthalene-2-carboxylic acid (CAS No: 92-70-6) was conducted on rabbits to assess its irritation potential in accordance with OECD Guide-line 405 "Acute Eye Irritation/Corrosion".

 

During the test, about 100mg of (0.1ml) of chemical was was moistened with Polyethylene Glycol 400 was installed into the eye of each of three rabbits for 24 hours and later eyes were rinsed.

 

Theeyes were examined after 1, 24, 48, 72 hours and on day 7 and 14 after application according to Driaze method.One hour after application, swelling and conjunctival injections as well as secretion (clear, tinted by the test substance) were observed in all animals. At 24, 48 and 72 hours, conjunctivitis and diffuse corneal opacities were found. One animal showed iritis at 24 and 48 hours. 7 days after the application, corneal vascularization was observed in all animals.

 

The Draize score was 1.1 out of a maximum of 4 for corneal opacity, 0.2 out of 2 for iritis, 1.9 out of 3 for conjunctivitis, and 1.3 out of 4 for conjunctival curling.

 

Therefore on the basis of observations and mean scores, the chemical3-hydroxynaphthalene-2-carboxylic acid (CAS No: 92-70-6)was considered to be irritating to rabbits’ eye with the risk of serious eye damage.