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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1974
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

1
Chemical structure
Reference substance name:
Disodium 2-[[4,5-dihydro-3-methyl-5-oxo-1-(4-sulphonatophenyl)-1H-pyrazol-4-yl]azo]benzoate
EC Number:
228-809-6
EC Name:
Disodium 2-[[4,5-dihydro-3-methyl-5-oxo-1-(4-sulphonatophenyl)-1H-pyrazol-4-yl]azo]benzoate
Cas Number:
6359-83-7
Molecular formula:
C17H14N4O6S.2Na
IUPAC Name:
disodium 2-[[4,5-dihydro-3-methyl-5-oxo-1-(4-sulphonatophenyl)-1H-pyrazol-4-yl]azo]benzoate
Test material form:
solid: particulate/powder

Test animals

Species:
rat
Strain:
other: Tif:RAI
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 90 - 100g
- Housing: goup cages (5 animals), Macrolon
- Diet (e.g. ad libitum): ad lib.
- Water (e.g. ad libitum): ad lib.
- Acclimation period: min. 5 d
- Fasting before gavage: 12 h

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 - 23°C
- Humidity (%): 50 - 60% rh
- Photoperiod (hrs dark / hrs light): 10 / 14

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Doses:
1000, 3000, 10000, 15000
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 d
- Frequency of observations and weighing: not specified
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 15 000 mg/kg bw
Based on:
test mat.
Remarks on result:
not determinable due to absence of adverse toxic effects
Mortality:
No mortality occured in any treatment
Clinical signs:
1000 mg/kg: reduction in spontaneous motility, muscular hypotonia, hyperreflexia, no symptoms after 24 h
3000 mg/kg: ditto, hypoventilation, staxia, no symptoms after 7 d
10000 mg/kg: ditto, dyspnoea, spasmophilia, tremor, cyanosis
15000 mg/kg: ditto, exophthalmos, no symptoms after 10 d
Body weight:
155 - 167g, no effect

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
The substance did not cause mortality in male/female rats up to the 15000 mg/kg bw. The LD50 (oral, gavage) in rat was determined to be >15000 mg/kg bw. The substance is not classifiable according to CLP criteria.