Benzoic acid, 2-hydroxy-5-[[4-[[4-[[8-hydroxy-7-[[4-[(8-hydroxy-3,6-disulfo-1-naphthalenyl)azo]-2-methoxy-5-methylphenyl]azo]-3,6-disulfo-1-naphthalenyl]amino]-6-(phenylamino)-1,3,5-triazin-2-yl]amino]phenyl]azo]-, pentasodium salt

Endpoint summary

Description of key information

Short-term toxicity on Daphnia magna:

EC50 (48h) > 100 mg/l

EC0 (48h) = 10 -100 mg/l

Toxicity to aquatic plants other than algae:

EC50 (7 d) > 482 mg/l of active ingredient, referred to the growth rate of frond number,

EC50 (7 d) > 482 mg/l of active ingredient, referred to the growth rate of dry weight.

Additional information

Data on aquatic toxicity of test item was available on fish, aquatic invertebrates, aquatic plants and microorganisms .

The test item is soluble in water, i.e. 4.38 g/l at 20 °C and pH 7.30 and it is not ready biodegradable.

Fish

Information from toxicity studies on fish with effect levels in terms of mortality were available. Effects on fish were noted at concentration of 820 mg/l and above 1000 mg/l. As no details on test method and procedure was available, this data was only taken as a qualitative indication of low toxicity to fish.

Aquatic invertebrates

Acute toxicity to daphnia magna was assessed in a 48 -hour study according to OECD 202. Effect concentrations in terms of immobilisation were determined:

EC0 (48h) = 10 - 100 mg/l

EC50 (48h) > 100 mg/l

This result was confirmed by an EC50 value of 209 mg/l, reported with few details on test procedure and results.

Algae

Due to its specific nature, this type of test was considered as not adequate to assess the potential toxicity of test substance, due to the influence of colouration of test substance on test outcome. Therefore, this study was not conducted.

Aquatic plants other than algae

The substance was tested for toxicity on freshwater aquatic plants, i.e.Lemna minor, according to OECD guideline 221 under static exposure conditions.

The EC50 values after 7 days resulted to be:

- > 482 mg/l of active ingredient, referred to the growth rate of frond number,

- > 482 mg/l of active ingredient, referred to the growth rate of dry weight.

Microorganisms

The potential toxicity of the substance to microorganisms was assessed using test samples with purity of 83, 70 to 82 %. No significant inhibition was noted upon exposure up to 2000, 10000 and 300 mg/l, respectively.

Such results gave an indication of low toxicity.

Justification for classification or non-classification

According to the CLP Regulation (EC n. 1272/2008), Part 4: Environmental Hazards, the substances can be classified for hazardous to the aquatic environment (fish toxicity) when the following criteria are met:

A )Acute (short-term) aquatic hazard

Category Acute 1:

96 hr LC 50 (for fish) ≤ 1 mg/l and/or, 48 hr EC 50 (for crustacea) ≤ 1 mg/l and/or, 72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l

B) Long-term aquatic hazard

(iii) Substances for which adequate chronic toxicity data are not available

Category Chronic 1:

96 hr LC 50 (for fish) ≤ 1 mg/l and/or 48 hr EC 50 (for crustacea) ≤ 1 mg/l and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l

and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

Category Chronic 2:

96 hr LC 50 (for fish) > 1 to ≤10 mg/l and/or 48 hr EC 50 (for crustacea) > 1 to ≤10 mg/l and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) > 1 to ≤10 mg/l (Note 2)

and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

Category Chronic 3:

96 hr LC 50 (for fish) > 10 to ≤ 100 mg/l and/or 48 hr EC 50 (for crustacea) > 10 to ≤ 100 mg/l and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) > 10 to ≤ 100 mg/l

and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

Category Chronic 4

Cases when data do not allow classification under the above criteria but there are nevertheless some grounds for concern. This includes, for example, poorly soluble substances for which no acute toxicity is recorded at levels up to the water solubility (note 4), and which are not rapidly degradable in accordance with section 4.1.2.9.5 and have an experimentally determined BCF ≥ 500 (or, if absent, a log K ow ≥ 4), indicating a potential to bioaccumulate, which will be classified in this category unless other scientific evidence exists showing classification to be unnecessary. Such evidence includes chronic toxicity NOECs > water solubility or > 1 mg/l, or other evidence of rapid degradation in the environment than the ones provided by any of the methods listed in section 4.1.2.9.5.

The EC50 value calculated for all species is greater that 100 mg/l therefore the substance is not classified for aquatic toxicity according to the CLP Regulation (EC n. 1272/2008).