Dicarbonyl(pentane-2,4-dionato-O,O')rhodium

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study does not follow modern guideline, but data are reasonaly well documented and scientifically acceptable. Limited reporting of test material (e.g. purity).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

Principles of method if other than guideline:

A range finding study using groups of two rats (one male, one female) at five dose levels was conducted to identify the highest dose at which no deaths occurred. In the main study, a further group of 10 rats (5 male, 5 female) was treated at this dose.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dicarbonyl(pentane-2,4-dionato-O,O')rhodium

EC Number:

238-947-9

EC Name:

Dicarbonyl(pentane-2,4-dionato-O,O')rhodium

Cas Number:

14874-82-9

Molecular formula:

C7H7O4Rh

IUPAC Name:

Dicarbonyl(pentane-2,4-dionato-O,O')rhodium

Details on test material:

- Name of test material (as cited in study report): CB.23 – Rhodium Dicarbonyl Acetylacetonate
- Substance type: Green metallic powder
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Lot/batch No.: no data
- Other: Supplied in a screw topped glass jar.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Olac, Shaws Farm, Blackthorn, Bicester, Oxon
- Age at study initiation: “young adult”
- Weight at study initiation: 170-235 g
- Fasting period before study: overnight
- Housing: 5 rats (of one sex) in polypropylene cages
- Diet (e.g. ad libitum):ad libitum oxoid maintenance diet supplied by Herbert C. Styles (Bewdley) Ltd
- Water (e.g. ad libitum): ad libitum
- Acclimation period:

ENVIRONMENTAL CONDITIONS
- Temperature (°C): “thermostatically controlled room”
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): “controlled lighting conditions”

IN-LIFE DATES: From: To: no data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

vegetable oil

Details on oral exposure:

A single dose was administed orally by stomach tube using a rubber catheter. Test material was delivered in a total volume of 10 ml vegetable oil.

Doses:

Range finding study: 25, 50, 200, 500 and 2000 mg/kg bw.
Main study: 50 mg/kg bw.

No. of animals per sex per dose:

Range finding study: one rat/sex/dose
Main study: five rats/sex/dose

Control animals:

no

Details on study design:

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- Duration of observation period following administration: 14 days- Frequency of observations and weighing: Animals were weighed immediately prior to dosing. The animals were examined immediately after dosing, four-hours after dosing, and then daily for 14 days for signs of toxicity.
- Necropsy of survivors performed: no
- Other examinations performed: none

Statistics:

No statistical analysis was performed.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

approximate LD50

Effect level:

> 50 - < 500 mg/kg bw

Mortality:

In the range-finding study, both the male and the female rats died (24 hrs and 4 days after dosing, respectively) at 2000 mg/kg bw. The females in the 500 and 200 mg/kg bw dose level groups died (at 4 days and 3 days after dosing, respectively). No deaths were seen at the 50 mg/kg bw dose level or below. In the main study, one of the group of five females died at the selected treatment level of 50 mg/kg bw. The remaining females and all five treated males survived the 14 day observation period.

Clinical signs:

other: Not reported.]

Gross pathology:

Not reported.]

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

The acute oral LD50 value of rhodium dicarbonyl acetylacetonate in rats was established to be within the range of 50-500 mg/kg bw.

Executive summary:

The acute oral toxicity of rhodium dicarbonyl acetylacetonate was assessed in rats, using a method equivalent to OECD Test Guideline 401. In a range finding study, groups of Sprague-Dawley rats (1/sex/group) were administered the test item at doses of 25, 50, 200, 500 or 2000 mg/kg bw by stomach tube. Both rats died in the 500 and 2000 mg/kg bw dose groups, within four days of treatment. The female rat treated at 200 mg/kg bw died three days post-treatment. Therefore, in the main study, groups of five rats/sex were administered a dose of 50 mg/kg bw. One female rat died three days after treatment. The remaining animals survived the 14 day observation period and were reported to show “no overt signs of toxicity”. 

 

The acute oral median lethal dose (LD50) of rhodium dicarbonyl acetylacetonate was determined to be between 50 and 500 mg/kg bw. Based on the results of this study, rhodium dicarbonyl acetylacetonate should be classified for acute oral toxicity (category 3) according to EU CLP criteria (EC 1272/2008).