Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-595-4 | CAS number: 97-62-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation, other
- Remarks:
- an in vivo skin sensitization (non-LLNA) study was performed based on ambigious results in the in vitro test battery
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23.03.2016 - 07.07.2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- The aim of the test was to assess the contact sensitizing potential of the test item in follow-up of 3 in vitro skin sensitisation studies that could not give a clear result. Structural similar substances gave stimulation indices (SI) > 3 which were considered not relevant for humans. For that reason, GPMT was performed in order to avoid false positive results.
Test material
- Reference substance name:
- 3-methylbutyl isovalerate
- EC Number:
- 211-536-1
- EC Name:
- 3-methylbutyl isovalerate
- Cas Number:
- 659-70-1
- Molecular formula:
- C10H20O2
- IUPAC Name:
- 3-methylbutyl 3-methylbutanoate
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- other: CrlOri:Ha
- Sex:
- male/female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: male: 255 - 282 g, female: 337 - 395 g
- Age at study initiation: male: 4 weeks, female: 5 weeks
- Housing: 1 animal in stainless wire mesh cages, 210W×350D×180H (mm)
- Diet: ad libitum, Pellet feed for experimental animals (Purina experimental diet for guinea pig 38065)
- Water: ad libitum, public tap water
- Acclimation period: 12 days
- Microbiological status of animals, when known: SPF
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20.0–23.8
- Humidity (%): 33.9–52.1
- Air changes (per hr): 10–15 times/h
- Photoperiod (hrs dark / hrs light): 12 hour light/dark cycle (7 AM to 7 PM via automated timer), 150-300 Lux
Study design: in vivo (non-LLNA)
Induction
- Route:
- intradermal and epicutaneous
- Vehicle:
- olive oil
- Concentration / amount:
- first induction 25% and second induction 100%
- Day(s)/duration:
- 48 hours
- Adequacy of induction:
- highest concentration used causing mild-to-moderate skin irritation and well-tolerated systemically
Challenge
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- 100%
- Day(s)/duration:
- 24 hours
- No. of animals per dose:
- 10
- Details on study design:
- PRELIMINARY TEST:
Intradermal Injection and topical application:
- Solvent: olive oil
- Concentration: 100, 50, 25, 12.5, 6.25, and 3.13%
- Amount animals: 2 x 2 (2 intradermal dosing and 2 topical application)
- Recordings: 48 h after treatment.
- Application method:
-- Sites: shoulder was clipped and shaved, 6 sites: 3 on the left and 3 on the right side
-- Concentration: 100, 50, 25, 12.5, 6.25 and 3.13%
-- Procedure: closed patch by means of an occlusive bandage
-- Recordings: 24 and 48h post application
-- Patches: 2×2 cm patch, animals were wrapped with adhesive tape and secured with surgical tape
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 2
First induction: Intradermal
Second induction and challenge: Dermal (topical application): on Day 7 after first induction
- Exposure period: 48 h
- Site: 2 x 4 cm clipped and shaved, application sites were pretreated with 0.5 mL of 10% SDS
- Concentration: first induction: 25%; second induction: 100%
- Test group:
1. WFI-FCA emulsion
2. 25% Test substance
3. 25% Test substance-FCA emulsion
- Control group:
1. WFI-FCA emulsion
2. Olive oil
3. Olive oil-FCA emulsion
B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge:
- Site: 2 x 2 cm clipped and shaved
- Concentrations:100%
- Evaluation (hr after challenge): 24 hours
- Test and control group: 100% test substance was applied to the left challenge sites of the test substance and control groups, while at the right challenge sites of the test substance and control groups olive oil was applied
EVALUATION
- Clinical signs: daily
- Body weight: on day 0, once weekly and on day 24
- Skin sensitisation: see scoring system
SCORING SYSTEM: Magnusson and Kligman
No reaction: 0
Scattered mild redness: 1
Moderate & diffuse redness: 2
Intense redness & swelling: 3
STATISTICAL ANALYSIS: Statistical analysis was not performed. Mean scores and values are presented. - Challenge controls:
- Olive oil
- Positive control substance(s):
- yes
- Remarks:
- 1-Chloro-2,4-dinitrobenzene (CDNB): A positive control group was not set in this study, a positive control study was conducted periodically
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 100%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 100%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- no indication of skin sensitisation
Any other information on results incl. tables
CLINICAL OBSERVATIONS: No abnormal signs or symptoms were observed in any animal of any group throughout the course of the study.
BODY WEIGHTS: All animals exhibited normal body weight gain.
POSITIVE CONTROL: The sensitization rate of CDNB was 100% and the sensitization grade was classified to be ‘V (Extreme)’. Consequently, the study was considered to be valid.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No animal showed an allergic response, the test article is not sensitising.
- Executive summary:
The aim of the current study was to assess the skin sensitizing potential of the test item, by means of a maximization test using guinea pigs according to OECD 406 and GLP.
In a preliminary test topical application did not reveal any evidence of adverse skin reactions, therefore, the induction sites in the main test for each group was pretreated with 10% sodium dodecyl sulfate prior to the second induction.
There were 2 groups, one for the test substance, this group consisted of 10 animals, while the second group was the control group made of 5 animals. In the test group, a concentration of 25% of the test substance was injected intradermally for the first induction. The second induction was conducted with 100% of the test substance. The sites were occluded for 48 hours, whereafter the challenge was conducted with 100% of the test substance. Hereafter, the sites were occluded for 24 hours. In the control group, the first and second inductions was done with olive oil and the challenges with the 100% of the test substance and olive oil.
Both in the control and the test group no skin reactions such as redness or swelling were observed in any animal at 24 and 48 hours after the challenge patch was removed. During the observation period, no abnormalities in clinical signs or body weight gain were observed in any animal of any group. Based on the result of this study, the test substance did not produce skin sensitization under the conditions of this study.
Therefore, the test article does not have to be classified as sensitising.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.