Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 290-924-2 | CAS number: 90294-40-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- see document in section 13
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 975
- Report date:
- 1975
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- (no untreated skin areas to serve as the control; exposure period is of 24 h; smaller area of application (i.e., approx. 2.5 cm2 gauze pad))
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Chromate(2-), [2,4-dihydro-4-[(2-hydroxy-4-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)]-, disodium
- EC Number:
- 274-386-6
- EC Name:
- Chromate(2-), [2,4-dihydro-4-[(2-hydroxy-4-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)]-, disodium
- Cas Number:
- 70209-87-9
- Molecular formula:
- C32H21CrN10O11S.2Na
- IUPAC Name:
- Disodium-[2,4-dihydro-4-[(2-hydroxy-4-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)]-chromate(2-)
1
Test animals
- Species:
- rabbit
- Strain:
- other: Russian
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 1.5 to 2 kg
- Housing: The animals were housed individually in V2A wire cages.
- Diet: Standard rabbit food - NAFAG, Gossau SG, rabbit food (ad libitum)
- Water: ad libitum
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- clipped
- Vehicle:
- other: polyethylene glycol (PEG 400)
- Controls:
- not specified
- Amount / concentration applied:
- - Amount(s) applied (volume or weight with unit): 500 mg
- Concentration (if solution): 50 % - Duration of treatment / exposure:
- 24 h
- Observation period:
- 24 and 72 h
- Number of animals:
- 6 (3 males and 3 females)
- Details on study design:
- PROCEDURE:
-The test was carried out according to the method given in the "Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the US Association of Food and Drug Officials (AFDO) which is similar to the OECD Guideline 404.
-2 d prior to the dermal application, six rabbits were shaved on the whole back and flanks with an electric clipper.
-The shaven skin on the left side was slightly scarified immediately before treatment.
-A gauze patch 2.5 x 2.5 cm was soaked with the test substance and immediately applied to the prepared skin. The patch was covered with a plastic film 5 x 5 cm, which was fixed to the body with adhesive tape
-Test substance was applied to each side in quantities of 500 mg. Before application a 50 % polyethylene glycol trituration (PEG 400) was made. The gauze patches were removed 24 h after the application. The reaction of the skin was appraised upon removal and 72 h after it.
SCORING SYSTEM:
Dermal irritation was graded in each animal according to the following scoring scale:
- Erythema and eschar formation:
0 no erythema
1 very slight erythema (barely perceptible)
2 well-defined erythema
3 moderate to severe erythema
4 severe erythema (beet redness) to slight eschar formation (injuries in depth)
- Edema formation:
0 no edema
1 very slight edema (barely perceptible)
2 slight edema (edges of area well-defined by definite raising)
3 moderate edema (raised approximately 1 millimeter)
4 severe edema (raised more than 1 millimeter and extending beyond area of exposure)
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- other: all animals
- Time point:
- other: 24, 72 h mean
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- other: for both intact and abraded skin
- Irritation parameter:
- edema score
- Basis:
- other: all animals
- Time point:
- other: 24, 72 h mean
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- other: for both intact and abraded skin
- Irritation parameter:
- primary dermal irritation index (PDII)
- Basis:
- mean
- Time point:
- other: all the ratings found after 24 and 72 h
- Score:
- 0
- Remarks on result:
- other: for both intact and abraded skin
- Irritant / corrosive response data:
- The primary irritation index as the measure of the acute irritation to the skin of rabbits was found to be 0.
- Other effects:
- none
Any other information on results incl. tables
Evaluation of the skin reactions:
Animal No. (sex) |
Skin Reaction |
24 h after application |
72 h after application |
||
Intact skin |
Scarified skin |
Intact skin |
Scarified skin |
||
1 (M) |
Erythema |
0 |
0 |
0 |
0 |
Edema |
0 |
0 |
0 |
0 |
|
2 (M) |
Erythema |
0 |
0 |
0 |
0 |
Edema |
0 |
0 |
0 |
0 |
|
3 (M) |
Erythema |
0 |
0 |
0 |
0 |
Edema |
0 |
0 |
0 |
0 |
|
4 (F) |
Erythema |
0 |
0 |
0 |
0 |
Edema |
0 |
0 |
0 |
0 |
|
5 (F) |
Erythema |
0 |
0 |
0 |
0 |
Edema |
0 |
0 |
0 |
0 |
|
6 (F) |
Erythema |
0 |
0 |
0 |
0 |
Edema |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Conclusions:
- Under the study conditions, the test substance was considered to be non-irritating to rabbit skin.
- Executive summary:
An in vivo study was conducted to evaluate the skin irritation potential of the analogue substance 2 (at ca. 75% purity) in Russian rabbits according to the method given in the Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the US Association of Food and Drug Officials (AFDO) which is similar to OECD Guideline 404 with few deviations.
In this study, 500 mg of test substance (i.e. 50% polyethylene glycol trituration (PEG 400)) was applied on a 2.5 cm2 gauze pad which was then applied to the abraded as well as intact skin of rabbits for 24 h under occlusive conditions. After the 24 h of application period, the dressing was removed and the intact and abraded application sites were assessed for oedema and erythema. A further assessment was made at 72 h.
No skin reaction was observed in any of the treated animal. The primary irritation index was calculated to be 0.
Under the study conditions, the analogue substance 2 was considered to be non-irritating to rabbit skin.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.