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EC number: 258-556-7 | CAS number: 53445-37-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-09-01 to 1992-09-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Cited as Directive 87/302/EEC, part C, p. 89 (Algal inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2,4(or 2,4,4)-trimethyladipic acid
- EC Number:
- 258-556-7
- EC Name:
- 2,2,4(or 2,4,4)-trimethyladipic acid
- Cas Number:
- 53445-37-7
- Molecular formula:
- C9H16O4
- IUPAC Name:
- 2,2,4-trimethylhexanedioic acid; 2,4,4-trimethylhexanedioic acid
- Details on test material:
- 2,2,4(or 2,4,4)-Trimethyladipic acid of Hüls AG, produced 25 March 1992. Sample from continuous production, storage tank FA 975.
Purity 90 % w/w, sample ID 3630/81389, sample No. 1324/920707
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Concentration of vehicle/solvent: 1 g test substance/l was dissolved in deionized water. No vehicle or solvent was used.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISMS
- Strain: Scenedesmus subspicatus (CHODAT (86.81 SAG))
- Source/supplier: Origin: Institute "Wasser-, Boden- und Lufthygiene", Berlin; further bred inhouse
- Laboratory culture: A preculture is seeded from a stock culture by transfer 3 days prior to begin of test. Test cultures are seeded from the latter.
- Initial cell concentration: approximately 20,000 cells/ml
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- no data available
- Test temperature:
- 24 +/- 2 °C
- pH:
- pH: 4.2-8.1 (beginning); 4.6-9.7 (end of test)
- Dissolved oxygen:
- no data available
- Nominal and measured concentrations:
- 6; 10; 20; 30; 60; 100 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Renewal of test solution: no
- Exposure vessel type: Sterile-aerated Erlenmeyer flasks on light benches
- Number of replicates: 5 (test substance) or 8 (control)
- Intensity of irradiation: approx. 7000-9000 lux, white
- Photoperiod: light
TEST PARAMETER
- Light absorption at 685 nm, related to cell density via calibration curve
STATISTICAL METHODS
- Probit analysis (Cavalli-Sforza, 1972) - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 25.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 41.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 67 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 29.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 107.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Value extrapolated
Any other information on results incl. tables
Cell density data:
Cells/ml after 0; 24; 48; 72 hours (mean)
Control: 2; 10; 50; 163 (x 10,000)
6 mg/l: 2; 11; 52; 164 (x 10,000)
10 mg/l: 2; 12; 57; 163 (x 10,000)
20 mg/l: 2; 12; 57; 138 (x 10,000)
30 mg/l: 2; 11; 52; 105 (x 10,000)
60 mg/l: 2; 5; 11; 24 (x 10,000)
100 mg/l: 2; 2; 3; 2 (x 10,000)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The lowest EC50 (based on cell numbers) of 2,2,4(or 2,4,4)-trimethyladipic acid for Desmodesmus subspicatus was calculated to be 41.6 mg/L indicating that the test substance may be harmful to algae.
- Executive summary:
The growth inhibition effects of 2,2,4(or 2,4,4)-trimethyladipic acid on Scenedesmus subspicatus were determined by Hüls AG (1992) according to EU-method C.3. Six concentrations ranging from 6 to 100 mg/L were tested over a period of 72 hours. The following endpoints were derived:
EbC50 (72 hours): 41.6 mg/L
ErC50 (72 hours): 56 mg/L
The study was assessed as "reliable without restrictions". However, pH values ranged from 4.2 to 9.7 and may have affected the results.
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