Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 228-589-1 | CAS number: 6300-50-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- other: Real Decreto 363/1995 (BOE núm. 133 de Junio de 1995)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent from the Urban Water Treatment Plant in Manresa (Spain)
No further details on inoculum given in the study report. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Solution 1:
KH2PO4 8.5 g/L H2O
K2HPO4 21.75 g/L H2O
Na2HPO4 2H2O 33.4g /L H2O
NH4Cl 0.5 g/L H2O
Solution 2: CaCl 2 2H2O 36.4 g/L H2O
Solution 3: MgSO 47H2O 22.5 g /L H2O
Solution 4: FeCl3 6H2O 0.25 g/L H2O
Add to 1000 mL of deionized water:
Solution 1 1 mL
Solution 2 1 mL
Solution 3 1 mL
Solution 4 1 mL
- Test temperature: 20 ± 2 ºC
- pH: not specified
- Aeration of dilution water: not specified
- Suspended solids concentration: not specified
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Erlenmeyer flasks (100 ml) with a cap, fully filled
- Number of culture flasks/concentration: 2
- Measuring equipment: not specified
- Details of trap for CO2 and volatile organics if used: not specified
- Other:
Preparation of stock and test solutions: A stock solution having a concentration of 1 g/L of the material presented (ref: Red P / Paper 8BLP (batch: PAL-025777)) was prepared. Therefore, 0.1 g of the presented material has been dissolved in 100 mL of water in a 100 mL Class A volumetric flask by shaking, so we have a stock solution of 1000 mg / L. To prepare the solution to be tested (5 mg / L), 10 mL of stock solution was diluted in 2 liters of inoculated culture medium.
The test containers have been filled with the solution to overflow, so that no bubbles of air remain inside the bottle.
Concentrations per test: 1 + substance for reference + blank
SAMPLING
- Sampling frequency: at day 0, 3, 7, 10, 14, 21, 28 (Number of measurements: 7)
- Sampling method: not specified
- Sterility check if applicable: not specified
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: not specified
- Toxicity control: not specified - Reference substance:
- acetic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 0 h
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0.9
- Sampling time:
- 3 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2.3
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 6
- Sampling time:
- 10 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 11.1
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 13.7
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 18
- Sampling time:
- 28 d
- Parameter:
- COD
- Value:
- 1.74 g O2/g test mat.
- Results with reference substance:
- Sodium acetate: 0,68 mg O2/mg
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The result obtained in this study indicates that the test item has a biodegradability of 18% at the initial concentration tested (5 mg / L) at 28 days of testing.
- Executive summary:
The determination of the biodegradability has been carried out after 28 days for the test substance DR 254 TEA salt following the OECD Guideline 301D (closed bottle test) (1992) together with the Spanish Royal Decree 363/1995 (BOE No. 133 of June 1995).
Biodegradation is defined as the decomposition of an organic compound by the biological action of microorganisms, which results in the disappearance of the original molecular structure and the formation of smaller organic molecules.The results are expressed as the percentage of biodegradation after 28 days.
Starting from a known concentration of the test substance (5 mg / L), monitoring is carried out by measuring the oxygen consumption at intervals of time over a period of 28 days.
Reference
Description of key information
There is one study available for the substance with CAS 64 683 -40 -5 (Direct Red 254 TEA salt) done according to the OECD 301D (readily biodegradability). This substance has the identical chemical molecular structure in respect to the anionic organic components than the substance with CAS 6300 -50 -1 (Direct Red 254 Na salt).
The study of biodegradation in water assigned as Key Study is the one conducted according to OECD guideline 301D on the substance with CAS 64 683 -40 -5 (Direct Red 254 TEA salt).
The result of 18 % of degradation after 28 days (< 60%), based on the oxygen consumption shows that the substance can not be classified as ready biodegradable.
Both substances CAS 64 683 -40 -5 (Direct Red 254 TEA salt) and CAS 6300 -50 -1 (Direct Red 254 Na salt) have the same anionic structure. This is expected the same biodegradability concerning this organic part.
Whereas the counterion of the substance CAS 6300 -50 -1 (Direct Red 254 Na salt) is the sodium, the one of the substance with CAS 64 683 -40 -5 (Direct Red 254 TEA salt) is the triethanolamine, which is susceptible to be biodegraded too. If the determined biodegradability of this substance, that has a greater contribution of organic structures to be degraded, is so low (18%) it is not expected that the substance with CAS 6300 -50 -1 (Direct Red 254 Na salt) could have a higher degree of biodegradation. Therefore, it is justified to consider that the substance with CAS 6300 -50 -1 will have at most the same degree of degradation of the substance with CAS 64683 -40 -5, that means "non readily biodegradable".
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.