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EC number: 266-797-4 | CAS number: 67633-96-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 June 2015 - 23 July 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: "Testing Methods for New Chemical Substances" Japan (March 31, 2011, No. 0331-7; March 29, 2011, No.5; No. 110331009; April 2, 2012 partial revision, No. 0402-1; March 28, 2012, No.2; No. 120402001)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- ThOD = 60.6 mg O2 per 30 mg test substance added
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: On-site sludge sampling was carried out at 10 locations in Japan (samples were from surface water and surface soil of rivers, lakes, and inland sea; rettun sludge from sewage plants)
- Storage length: sampling period: May, 2015, initiation date of use: June 19, 2015
- Preparation of inoculum for exposure: The activated sludge, which was cultivated for 19 hours after the synthetic sewage was added, was used. The synthetic sewage was prepared according to the following method; glucose, peptone, and potassitun dihydrogenphosphate were dissolved in purified water, and the pH of the solution was adjusted to 7.0 ± 1.0.
- Concentration of sludge: 3050 mg/L suspended solid in the activated sludge
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- BOD is measured
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD 301C
- Test temperature: 25 +/- 1 ºC
- pH: at start: 5.9-7.0, at the end: 6.2-7.3
- Aeration of dilution water: not indicated
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption measuring apparatus
- Number of culture flasks/concentration: 3 (sludge + test item)
- Method used to create aerobic conditions: stirring
- Measuring equipment: a closed system oxygen consumption measuring apparatus, which uses Soda lime No.1 for absorption of carbon dioxide
SAMPLING
- Sampling frequency: continuously
- Sampling method: BOD of the test solutions was measured continuously
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, one replicate
- Abiotic sterile control: water + test item: 1 replicate
- Reference control: sludge + aniline: 1 replicate
- toxicity control: no - Reference substance:
- aniline
- Remarks:
- ThOD = 72.3 mg O2 per 30 mg substance added
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 96 - 105
- Sampling time:
- 28 d
- Details on results:
- average % biodegradation by BOD was 101
average % biodegradation by DOC was 98
average % decrease of test item (by GC) was 100
At day 7 and day 14, the % degradation of the test substance was already 73-78 and 89-95, resp. - Validity criteria fulfilled:
- yes
- Remarks:
- see overall remarks
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance showed 101% biodegradation in the modified MITI test (OECD 301C) and is considered readily biodegradable.
- Executive summary:
The ready biodegradability of Liffarome was investigated in a study conducted in accordance with OECD 301C and GLP. The concentration tested was 100 mg/L test substance, with an activated sludge concentration of 30 mg/L. The test substance biodegraded for 76%, 93% and 101% after 7, 14 and 28 days, respectively, and is thus considered to be readily biodegradable. Cis-hexenol and methanol were detected as remaining metabolites.
Reference
Test solution (water +test item)
From the results of the quantitative analyses in the test solution after 28 days, the percentage residue of the test item was 10%, and the percentage production of cis-3-hexen-1-ol and methanol were 86% and 86%, respectively. It shows that the test item was hydrolyzed. 'The mass balances, which were calculated from these analytical results, the cis-3-hexen-1-ol part and methanol part were 96% and 96%, respectively. These results show that no other converted products except cis-3-hexen-1 -ol, methanol and carbon dioxide were produced. In addition, it was considered that residual amount of DOC derived from the test item methanol, and cis-3-hexen1-ol which were dissolved in the test solution.
Description of key information
The ready biodegradability of Liffarome was investigated in a study conducted in accordance with OECD 301C and GLP. The concentration tested was 100 mg/L test substance, with an activated sludge concentration of 30 mg/L. The test substance biodegraded for 76%, 93% and 101% after 7, 14 and 28 days, respectively, and is thus considered to be readily biodegradable. Cis-hexenol and methanol were detected as remaining metabolites.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
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