4-(1,1-dimethylethyl)cyclohexyl acrylate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1981-08-04 to 1981-08-20

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

Based on substance loss (by weight) the concentration was app. 0.1 mg/L, but the calculated saturated vapour concentration is 51.7 mg/L

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Principles of method if other than guideline:

The test was performed in principle as described in OECD test guideline 403. It demonstrates the toxicity of an atmosphere saturated with vapors of the volatile components of a test substance at 20 °C. Young adult laboratory rats, 3 per sex, were exposed sequentially to the vapors generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 7 h. The exposure was subsequently repeated in the same manner. No analytical determination of the atmosphere concentrations was performed. Group-wise documentation of clinical signs was performed over a 14 day study period. Afterwards, the animals were killed and examined by gross pathology. The nominal concentration was calculated as quotient of the amount of test substance weight loss during exposure, and the amount of air used during exposure.

GLP compliance:

no

Test type:

other: Inhalation hazard test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-(1,1-dimethylethyl)cyclohexyl acrylate

EC Number:

282-104-8

EC Name:

4-(1,1-dimethylethyl)cyclohexyl acrylate

Cas Number:

84100-23-2

Molecular formula:

C13H22O2

IUPAC Name:

4-tert-butylcyclohexyl prop-2-enoate

Details on test material:

- Name of the test substance (as cited in study report): 4-tertiaer-Butylcyclohexylacrylat
- Substance type: organic
- Physical state: liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Wiga, Sulzfeld, FRG
- Age at study initiation: 7 - 10 weeks
- Weight at study initiation: males: 180 - 250 g; females: 180 - 250 g
- Fasting period before study: no
- Housing: groups of 3 animals per sex (Wire cage (Becker, D III))
- Diet: ad libitum, Herilan MRH (EGGERSMANN KG, Rinteln, FRG) in the form of pellets
- Water: ad libitum, tap water
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 55 ± 5
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
The product was introduced to a height of 5 cm into a glass bottle (generator) with a sintered glass disk (pore-size 90 - 150 µm, diameter 30 mm), and the weight was determined.
The generator containing the product was placed in a water bath maintained at 20 °C by a thermostat, and a stream of 200 L/h compressed air was supplied to a downstream mixing chamber. The mixture of air and test substance generated in this way was passed through a glass distributor to 6 glass tubes in which 3 male and 3 female animals had been placed. The emerging mixtures of test substance and air were exhausted.
The temperature in the exposure apparatus was between 19 and 25 °C. After 30 minutes, the generator was replaced by a new one containing fresh test substance as described above. This generator was then used for the remainder of the test.

Determination of the nominal concentration:
The amount of test substance used was determined by reweighing the generators. The nominal concentration was calculated from the amount of test substance consumed and the air volume.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

7 h

Concentrations:

0.86 mg/L

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: clinical examinations each work day and lethality each day.
- Necropsy of survivors performed: yes (gross pathology)

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

other: IHT

Remarks on result:

other: No mortality was observed after 7 h exposure

Mortality:

No mortality was observed.

Clinical signs:

other: Restlessness was observed during exposure. No clinical signs were observed after exposure and during the post exposure observation period.

Gross pathology:

No pathological findings were noted.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification