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EC number: 240-596-1 | CAS number: 16529-56-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not stated
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to OECD 402. Study performed before GLP statemnt. Substance purity and composition are not stated.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- : Occlusive condition, no details on test material and on test animals
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- no
- Remarks:
- Study performed before GLP statment
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-methyl-3-butenenitrile
- EC Number:
- 240-596-1
- EC Name:
- 2-methyl-3-butenenitrile
- Cas Number:
- 16529-56-9
- Molecular formula:
- C5H7N
- IUPAC Name:
- 2-methylbut-3-enenitrile
- Details on test material:
- - Name of test material (as cited in study report): 2-methyl 3 butenenitrile (2M3BN)
- Substance type: no data
- Physical state: colorless liquid
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: no data
- Isomers composition: no data
- Purity test date: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: no data
- Other: no additional data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: IFFRA CREDO
- Age at study initiation: no data
- Weight at study initiation: between 150 and 230 g.
- Fasting period before study: no data
- Housing: in cage of 37.5 x 23.5 x 16 cm, 2 or 5 rats per cage, with a dusted and sterilized and sawdust litter
- Diet (e.g. ad libitum): IFFARAT food ad libitum
- Water (e.g. ad libitum): ad libitum except a 17/18 hours diet before treatment
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22° ± 1°C
- Humidity (%): 50 ± 10%
- Air changes (per hr): 8 per hour
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: no data
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: no data
- % coverage: no data
- Type of wrap if used: silver foil and adhesive tape
REMOVAL OF TEST SUBSTANCE
- Washing (if done): no
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0, 1.85, 2.16, 2.47, 3.09 mL/kg
- Concentration (if solution): no
- Constant volume or concentration used: no
- For solids, paste formed: no
VEHICLE
No vehicle - Duration of exposure:
- 24 hours
- Doses:
- 0, 1500, 1750, 2000 and 2500 mg/kg
- No. of animals per sex per dose:
- 5 animals/sex/dose
- Control animals:
- yes, concurrent no treatment
- Details on study design:
- - Duration of observation period following administration: 1, 2 and 6 hours after exposure and daily until 14 days of observation.
- Frequency of observations and weighing: on day 0, 1, 2, 4, 7 and 14 after exposure
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- Probit method, Litchfield and Wilcoxon method and Arcsinus method were used.
Results and discussion
- Preliminary study:
- In a preliminary study, 2-methyl-3-butenenitrile was admistered by dermal way to 4 groups of 4 Sprague-Dawley rats (2 males and 2 females) at doses of 100, 500, 1000 and 2000 mg/kg. (See table 7.2.3/1)
At doses of 100 and 500 mg/kg, the test subtance was preliminary diluted in an aqueous dispersion of arabic gumm 10%.
Animals were observed and mortality noted at 1, 2 and 6 hours post-exposure and for 14 days (See table 7.2.3/3).
0% mortality was observed at dose level of 100, 500 and 1000 mg/kg, and 50% mortality was observed at dose level of 2000 mg/kg.
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 849 mg/kg bw
- 95% CL:
- >= 1 533 - <= 2 231
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 560 mg/kg bw
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2 368 mg/kg bw
- Mortality:
- See table 7.2.3/3
At 1500 mg/kg, there was 0% of mortality.
At 1750 mg/kg, there was 20% of mortality.
At 2000 mg/kg, there was 60% of mortality.
At 2500 mg/kg, there was 70% of mortality. - Clinical signs:
- other: At 1500 mg/kg: Reduced spontaneous activity and apathy were observed at 1 hour post-exposure and persist on day 1 post-exposure. On day 2 post-exposure, spontaneous activity still be reduced but on day 4 post-exposure, survivors go back to a normal behavi
- Gross pathology:
- At all dose levels, in animals dead during experiment, haemorrhagic lungs, haemorragic thymus gland, ulcerated stomach and irritated intestine were observed at autopsy.
When survivors were sacrified, no macroscopic abnormalities were detected at all dose levels. - Other findings:
- At all doses tested, no irritation was observed at the application site.
Any other information on results incl. tables
Table 7.2.3/3: Preliminary study results of dermal acute toxicity on rats
Administration |
Animals |
Cumulated mortality |
|||||||||||
Dose mg/kg |
Volume mL/kg |
Concentration (%) |
Weight (g) |
Number |
1 hr |
2 hr |
6 hr |
1d |
2d |
4d |
7d |
14d |
% |
100 |
1.00 |
10 |
M 168 F 167 |
2 2 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 |
500 |
5.00 |
10 |
M 225 F 185 |
2 2 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 |
1000 |
2.16 |
Undiluted |
M 229 F 179 |
2 2 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 |
2000 |
2.47 |
M 179 F 175 |
2 2 |
0 0 |
0 0 |
0 0 |
1 1 |
1 1 |
1 1 |
1 1 |
1 1 |
50 |
Table 7.2.3/4: Dermal acute toxicity on rats
Administration |
Animals |
Cumulated mortality |
|||||||||||
Dose mg/kg |
Volume mL/kg |
Concentration |
Weight (g) |
Number |
1 hr |
2 hr |
6 hr |
1d |
2d |
4d |
7d |
14d |
% |
Control |
- |
- |
M 173 F 176 |
5 5 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 0 |
0 |
1500 |
1.85 |
Undiluted |
M 169 F 177 |
5 5 |
0 0 |
0 0 |
0 0 |
1 0 |
1 0 |
2 0 |
2 0 |
2 0 |
20 |
1750 |
2.16 |
M 174 F 179 |
5 5 |
0 0 |
0 0 |
0 0 |
2 0 |
2 1 |
3 2 |
4 2 |
4 2 |
60 |
|
2000 |
2.47 |
M 173 F 175 |
5 5 |
0 0 |
0 0 |
0 0 |
3 1 |
3 1 |
4 2 |
4 2 |
4 2 |
60 |
|
2500 |
3.09 |
M 168 F 177 |
5 5 |
0 0 |
0 0 |
0 0 |
3 0 |
5 0 |
5 0 |
5 1 |
5 2 |
70 |
Table 7.2.3/5: Body weight evolution after exposure of female Sprague-Dawley rats to 2 -methyl-3 -butenenitrile
Dose (mg/kg) |
Mean weight (g) |
|||||
Day 0 |
Day 1 |
Day 2 |
Day 4 |
Day 7 |
Day 14 |
|
Control |
176 |
164 |
174 |
184 |
203 |
223 |
1500 |
177 |
161 |
163* |
170* |
192* |
220 |
1750 |
179 |
162 |
158* |
174 |
192 |
217 |
2000 |
175 |
159 |
156* |
161 |
191 |
206 |
2500 |
177 |
156 |
158* |
161* |
179 |
217 |
*Significant at threshold 95%
Table 7.2.3/5: Body weight evolution after exposure of male Sprague-Dawley rats to 2 -methyl-3 -butenenitrile
Dose (mg/kg) |
Mean weight (g) |
|||||
Day 0 |
Day 1 |
Day 2 |
Day 4 |
Day 7 |
Day 14 |
|
Control |
173 |
154 |
170 |
187 |
217 |
270 |
1500 |
169 |
145 |
146 |
148 |
186 |
243 |
1750 |
174 |
156 |
151 |
151 |
185 |
236 |
2000 |
173 |
149 |
145 |
153 |
184 |
231 |
2500 |
168* |
150 |
- |
- |
- |
- |
*Significant at threshold 95%
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category IV
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under the test conditions of this study, the LD50 combined was 1849mg/kg bw, and the LD50 for males was 1560 mg/kg bw. thus 2-methyl-3-butenenitrile was considered in Category 4 (H312: Harmful in contact with skin) according to the Regulation 1272/2008/EC and as harmful in contact with skin (Xn, R21) according to the Directive 67/548/EEC.
- Executive summary:
In an acute dermal toxicity study, 2-methyl-3-butenenitrile was administered by dermal route to 5 groups of 10 Sprague-Dawley rats (5 males and 5 females) at dose levels of 0, 1500, 1750, 2000 and 2500 mg/kg, undiluted. This study was equivalent or similar to OECD 402 guideline. A preliminary study was performed. Animals were observed and mortality was noted at 1, 2 and 6 hours post-exposure and for 14 days, and they were weighed on day 0, 1, 2, 4, 7 and 14.
At 1500, 1750, 2000 and 2500 mg/kg, there was respectively 20, 60, 60 and 70% of mortality. No death was observed in the control group with vehicle alone applied.
At 1500 mg/kg, reduced spontaneous activity and apathy were observed at 1 hour post-exposure and persist on day 1 post-exposure. on day 4 post-exposure, survivors got back to a normal behaviour. At 1750 and 2000 mg/kg, spontaneous activity was reduced or null, apathy and ptosis were observed from 1 hour post-exposure and up to day 6 post-exposure. At the highest dose, the same signs as at lower dose levels were observed. At all doses tested, no irritation was observed at the application site.
For male, significant weight decrease was observed at 1500 mg/kg and at 2500 mg/kg, but at 14 day post-exposure, animals got back to their normal body weight. For females, a significant bodyweight decrease was observed at all dose levels, but on day 14 post-exposure, no decrease was oberved anymore.
At all dose levels, in animals dead during experiment, haemorrhagic lungs, haemorragic thymus gland, ulcerated stomach and irritated intestine were observed at autopsy. When survivors were sacrified, no macroscopic abnormalities were detected at all dose levels.
Under the test conditions of this study, LD50 combined is 1849 mg/kg bw (1532 -2231 mg/kg bw) and the LD50 for males was 1560 mg/kg bw. Therefore, 2 -methyl-3 -butenenitrile is considered in Category 4 (H312: Harmful in contact with skin) according to the Regulation 1272/2008/EC and as harmful in contact with skin (Xn, R21) according to the Directive 67/548/EEC.
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