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EC number: 200-562-9 | CAS number: 63-68-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study. The study itself was regarded as reliable without restrictions (Kl.1). However, according to the "Prctical guide 6: How to report read-across and categories" the maximum score for read-across is 2.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- DL-methionine
- EC Number:
- 200-432-1
- EC Name:
- DL-methionine
- Cas Number:
- 59-51-8
- Molecular formula:
- C5H11NO2S
- IUPAC Name:
- methionine
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CD / Crl: CD
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: males 45 days, females 56 days
- Weight at study initiation: males 221-241 g, females 200-217 g
- Housing: individually or X animals/cage
- Diet: Feeding was discontinued approx. 16 hours before exposure
- Water: ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C +/- 3°C
- Humidity (%): 55% +/- 15%
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- The study was carried out using a dynamic inhalation chamber (air changes/h ( >= 12 times)) with a nose-only exposure of the animals according to KIMMERLE & TEPPER. The equipment consists of a cylindrical exposure chamber (volume 40 L) which holds a maximum of 20 animals in pyrex tubes at the edge of the chamber in a radial position.
The dust of the test item was generated with a rotating brush dust generator.
Concentration (mg/L air): 5.25
Air flow entrance (l/h): 900
Air flow exit (l/h): 800
Air change (changes per hour): 22.5
ln the inhalation chamber, close to the animals' noses, the particlas had a mass median aerodynamic diameter (MMAD) of 2.207 µm as determinedwith a cascade impactor. The Geometric Standard Deviation (GSD) of the MMAD was calculated as 3.382. The geometric mean diameter (d50) of the
supplied test substance was 3.2403 µm as determined by the sponsor. - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 5.25 ±0.25 mg D,L-Methionine/L air
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- After completion of exposure, the animals were observed for a period of 14 days.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC0
- Effect level:
- > 5.25 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 5.25 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- No mortality occurred.
- Body weight:
- The animals gained the expected weight throughout the study period.
- Gross pathology:
- Necropsy of all animals was carried out and all gross pathological changes were recorded. No abnormalities were detected at necropsy.
Histopathology was not carried out as no lesions were observed macroscopically.
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- Under the present test conditions, the LC0 and LC50-values for CD® rats following inhalation of D,L-Methionine for 4 hours were determined as
follows (actual concentrations):
LC0 males: > 5.25 mg/L air for 4 hours
females: > 5.25 mg/L air for 4 hours
LC50 males: > 5.25 mg/L air for 4 hours
females: > 5.25 mg/L air for 4 hours
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