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EC number: 254-599-0 | CAS number: 39711-79-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 2.04 cm
- Weight at study initiation (mean and range, SD): average 0.084 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 185 mg of CaCO3
- Test temperature:
- 23 °C
- pH:
- 7.1
- Dissolved oxygen:
- 7.0 mg/l
- Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Mortality was observed
- Sublethal observations / clinical signs:
9.1. Mortality and symptoms
Nominal Concentration
Number of fish
Mortality (cumulated) after
(mg/l)
3 h
6 h
24h
48h
72h
96h
V1
V1
V1
V1
V1
V1
Control
8
-
-
-
-
-
-
100
8
-
-
1
2
2
2
Visible Symptoms:
Nominal Concentration
Number of fish
3 h
6 h
24h
48h
72h
96h
(mg/l)
V1
V1
V1
V1
V1
V1
Control
8
NS4
NS4
NS4
NS4
NS4
NS4
100
8
NS4
NS4
M3
M2
M2
M2
Explanation of symptoms:
A: apathy
N: Narcotic-like state
S: Surfacing behaviour
C: Swimming near water surface
M: Slow movements compared to control NS: No abnormal symptomsS: Surfacing behaviour
L: Loss of equilibrium SM: Shimming movement H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
E: Exopthalamos SC: Schooling Effect
8.2. pH and oxygen content:
Nominal
Number of fish
pH readings after
24h
48h
72h
96h
Concentration
(mg/l)
V1
V1
V1
V1
Control
8
7.4
7.5
7.6
7.7
100
8
7.9
8.0
8.1
8.2
Nominal
Number of fish
Oxygen content (mg/L) after
Concentration
(mg/l)
24h
48h
72h
96h
V1
V1
V1
V1
Control
8
6.7
6.6
6.5
6.4
100
8
6.5
6.4
6.3
6.3
9.3. Test temperature
Nominal
Number of fish
Temperature (°C) after
Concentration
(mg/l)
24h
48h
72h
96h
V1
V1
V1
V1
Control
8
23.9
23.8
24.1
23.8
100
8
23.9
23.8
24.1
23.8
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test substance on Zebra Fish Danio rerio was determine to be 100 mg/l
- Executive summary:
Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations 100 mg/l, LC50 was determine to be 100 mg/l. Mortality was observed at 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.
Reference
Description of key information
Short-term toxicity to fish:
Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations 100 mg/l, LC50 was determine to be 100 mg/l. Mortality was observed at 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Short-term toxicity to fish:
Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations 100 mg/l, LC50 was determine to be 100 mg/l. Mortality was observed at 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.
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