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EC number: 607-566-3 | CAS number: 25137-84-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 04st 2000 - March 27st 2001
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 2'DEOXY-5-ETHYL-URIDINE, 3', 5'-BIS (4- CHLOROBENZOATE)
- Description : white crystalline powder
- Lot number : β-CEDU 2/98-G
- Date received : 06 December 2000
- Storage conditions : ambient conditions - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from the aeration tank of the facility’ s sewage treatment plant.
- Preparation of inoculum for exposure: Coarse particles were removed by filtration through a fine sieve and the sample was then centrifuged at 1100 g for 10 minutes. The resulting supernatant was discarded. The pellet of sludge was washed with the mineral medium and centrifuged for a second time. The concentrated sludge was then suspended in mineral medium to yield a concentration of 3 g suspended solids/l. This was aerated until required.
- Water filtered: no - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium acc. to OECD 301 F
- Additional substrate: No
- Test temperature: 20°C
- pH: 6 – 8.5
- pH adjusted: no
TEST SYSTEM
- Culturing apparatus: Incubator
- Number of culture flasks/concentration: 6
SAMPLING
- Sampling frequency: daily
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, containing inoculum only.
- Abiotic sterile control: No
- Toxicity control: yes, containing test item, reference item and inoculum
- Positive control: yes, containing reference item and inoculum
STATISTICAL METHODS:
At each time point, the oxygen uptake (mg/l) of the test item was calculated as the mean of the two replicate flasks and corrected against uptake of the inoculum control (blank) at the same time point. Uptake of the inoculum control was similarly calculated as the mean of the two replicate flasks. The value obtained was divided by the concentration of test item in the flask to yield the Biochemical Oxygen Demand (BOD) expressed as mg oxygen/mg test item.
The percentage (biodegradation) was calculated from the observed BOD relative to the ThOD for the substance. Calculation was performed using the ThOD (NH4) as low degradation of the test item was observed. - Reference substance:
- other: sodium benzoate
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 20
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The results of this study indicate that the test item 2’deoxy-5-ethyl-uridine,3’,5’-bis(4-chlorobenzoate) is not readily biodegradable in the manometric respirometry test.
- Executive summary:
The ready biodegradability of 2’deoxy-5-ethyl-uridine,3’,5’-bis(4-chlorobenzoate) in an aerobic aqueous medium was assessed according to OECD Guideline for the testing of Chemicals No. 301F.
A measured volume of inoculated mineral medium, containing the test item at a nominal concentration of 100 mg/l as the nominal sole source of organic carbon, was stirred in a closed flask at constant temperature for 28 days. Evolved carbon dioxide was adsorbed and pressure changes measured by a manometer. Oxygen consumption by the test item was expressed as a percentage of the Theoretical Oxygen Demand (ThOD). The validity of the test System was assessed by concurrent use of a positive control reference item (sodium benzoate).
The observed low oxygen consumption and the derived BOD (Biochemical Oxygen Demand) indicate that no significant degradation has occurred. Values obtained with sodium benzoate, resulted in calculated biodegradation in excess of 60% of the theoretical value demonstrating validity of the test System in assessing biodegradability. The toxicity control (test and reference items combined) indicated that the test item was slightly inhibitory to the test System. Measured pH at the start and end of the 28 day test period was within specified limits.
Reference
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of 2’deoxy-5-ethyl-uridine,3’,5’-bis(4-chlorobenzoate) in an aerobic aqueous medium was assessed according to OECD Guideline for the testing of Chemicals No. 301F.
A measured volume of inoculated mineral medium, containing the test item at a nominal concentration of 100 mg/l as the nominal sole source of organic carbon, was stirred in a closed flask at constant temperature for 28 days. Evolved carbon dioxide was adsorbed and pressure changes measured by a manometer. Oxygen consumption by the test item was expressed as a percentage of the Theoretical Oxygen Demand(ThOD). The validity of the test System was assessed by concurrent use of a positivecontrol reference item (sodium benzoate).
The observed low oxygen consumption and the derived BOD (Biochemical Oxygen Demand) indicate that no significant degradation has occurred. Values obtained with sodium benzoate, resulted in calculated biodegradation in excess of 60% of the theoretical value demonstrating validity of the test System in assessing biodegradability.
The toxicity control (test and reference items combined) indicated that the test item was slightly inhibitory to the test System. Measured pH at the startand end of the 28 day test period was within specified limits.
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