Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 940-332-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 19, 2014 - March 25, 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Biphenyl-2-yl-(9,9-dimethyl-9H-fluoren-2-yl)-(9,9’-spirobifluoren-2-yl)-amine
- EC Number:
- 700-681-0
- Molecular formula:
- C52H37N
- IUPAC Name:
- Biphenyl-2-yl-(9,9-dimethyl-9H-fluoren-2-yl)-(9,9’-spirobifluoren-2-yl)-amine
Constituent 1
- Specific details on test material used for the study:
- Designation: Art. 139222
Synonym: HTM-081
Batch: OS11008922
Purity (HPLC): >99.95% (Area %)
Appearance: Pale yellow powder without visible impurities
Water solubility: 1.7e-12 mg/L
Released until: August 15, 2016
Storage: Tightly closed, dark at room temperature (15 to 25°C)
Sampling and analysis
- Analytical monitoring:
- no
- Remarks:
- The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (<1.7e-12 mg/L) the compound cannot be detected with standard analytical methods.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Vehicle:
Nutrient (mg/L)
NH4Cl 15.0
MgCl2 * 6H2O 12.0
CaCl2 * 2H2O 18.0
MgSO4 * 7H2O 15.0
KH2PO4 1.60
FeCl3 * 6H2O 0.0640
Na2EDTA * 2H2O 0.100
H3BO3 0.185
MnCl2 * 4H2O 0.415
ZnCl2 0.00300
CoCl2 * 6H2O 0.00150
CuCl2 * 2H2O 0.00001
Na2MoO4 * 2H2O 0.00700
NaHCO3 50.0
- pH: 7.86
- Total hardness: 24 mg/L as CaCO3 (calculated)
Preparation of the Test Item:
A stock preparation with a test item concentration of 100 mg/L was freshly prepared. For that purpose, the test item was weighed in a calibrated flask and vehicle was added. The preparation was treated in an ultrasonic device for 30 minutes. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Then the formulation was passed through a filter membrane (pore size: 0.2 µm). The filtrate was used for the study.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Unicellular green algae
- Strain: Desmodesmus subspicatus
- Source (laboratory, culture collection): The test system used was Desmodesmus subspicatus, strain 61.81 SAG (Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen, Germany). This strain was further cultivated in the laboratories of the test facility.
- Method of cultivation: The permanent algae cultures, the pre-culture and the algae cultures of the study were cultivated in an air-conditioned room with a water temperature of 21.0 – 24.0°C, controlled at
± 2°C. For cultivation, 300 mL Erlenmeyer flasks filled with 100 mL algae suspension were covered with air permeable stoppers (Heinz Herenz Medizinalbedarf GmbH, Hamburg, Germany). The cultures were shaken continuously at about 120 rpm (Universalschüttler SM25, Edmund Bühler GmbH, Hechingen, Germany) and lit between 4440 and 8880 Lux. Fluorescent tubes (Lumilux T5 nws FLH1 HO 80W/840, Osram GmbH, München, Germany) installed above the flasks served for lighting.
ACCLIMATION
- Acclimation period: 72h
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- -
Test conditions
- Hardness:
- 24 mg/L as CaCO3 (calculated)
- Test temperature:
- 22.4-23.1°C
- pH:
- 7.68 - 8.62
The pH in the control group did increase more than 1.5 units during the test. This did not influence the outcome of the study. - Nominal and measured concentrations:
- Nominal concentrations: 100.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks
- Type: open
- Material, size, headspace, fill volume: 300 mL Erlenmeyer glas flasks filled with 100 mL cell suspension
- Aeration: no
- Initial cells density: 10 000 cells per ml
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water according to OECD TG 201
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Light intensity and quality: The light intensity measured immediately before the start of the study was 6995 Lux ± 5.6% and 7119 Lux ± 8.3% at the end of the study. Thus, the light intensity was in the anticipated range of 4440 – 8880 Lux within ± 15% from the average.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
- Nominal test concentration: 100 mg/L - Reference substance (positive control):
- no
- Remarks:
- The accuracy and reliability of the test method is demonstrated periodically as recommended by OECD Guideline No. 201 and the Council Regulation (EC) No. 761/2009. Therefore, potassium dichromate (Art. 104864) was tested as positive control (Gado, 2014).
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: EC10 >1.7e-12 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: EC20 >1.7e-12 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: EC50 >1.7e-12 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: EC10 >1.7e-12 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: EC20 >1.7e-12 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: EC50 >1.7e-12 mg/L
- Details on results:
- The EC values exceeded the water solubility of 1.7e-12 mg/L (nominal >100 mg/L) and, thus, could not be determined in this study.
- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Unusual cell shape: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no - Results with reference substance (positive control):
- The accuracy and reliability of the test method is demonstrated periodically as recommended by OECD Guideline No. 201 and the Council Regulation (EC) No. 761/2009. Therefore, potassium dichromate (Art. 104864) was tested as positive control (Gado, 2014).
Under the conditions of the present study, the test item Art. 104864 (Potassium dichromate) showed 72h EC50 of 1.03 mg/L for growth rate and 0.53 mg/L for yield. The results obtained in this study are similar to results of a ring test mentioned in DIN EN ISO 8692.
Any other information on results incl. tables
Objective
The objective of this study was to evaluate the influence of the test item Art. 139222 (HTM-081) on the growth and growth rate of the green algae species Desmodesmus subspicatus.
Study Design
The study design included one control group and one test item group with six replicates, each containing 100 mL reconstituted water or test medium and about 10000 cells/mL at the start of the experimental phase. The study was performed as a limit test in an open static test system. The algae were exposed to a filtrate of nominal 100 mg/L. The growth of the algae was calculated after 24, 48, and 72 hours exposure in the test item group and control group.
Results
The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (1.7e-12 mg/L), the compound cannot be detected with standard analytical methods and the development of an analytical method with a sufficiently low detection and quantification limit is complex.
Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.
An aqueous preparation of a nominal concentration of 100 mg/L of Art. 139222 (HTM-081) revealed no toxic effect in Desmodesmus subspicatus.
The following EC values (Growth rate and Yield) were determined:
Parameter (0 -72h) | Growth Rate | Yield |
EC10 [mg/L] 95% confidence interval |
>1.7e-12 mg/L (nominal >100 mg/L) n.d. |
>1.7e-12 mg/L (nominal >100 mg/L) n.d. |
EC20 [mg/L] 95% confidence interval |
>1.7e-12 mg/L (nominal >100 mg/L) n.d. |
>1.7e-12 mg/L (nominal >100 mg/L) n.d. |
EC50 [mg/L] 95% confidence interval |
>1.7e-12 mg/L (nominal >100 mg/L) n.d. |
>1.7e-12 mg/L (nominal >100 mg/L) n.d. |
Conclusion
Under the conditions of the present study, an aqueous solution of 100 mg/L of Art. 139222 (HTM-081) in an open static system revealed no aquatic toxicity.
The EC50 (0-72h) for Growth rate and Yield were >1.7e-12 mg/L (nominal >100 mg/L) and, thus, could not be determined in this study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study, an aqueous solution of 100 mg/L of Art. 139222 (HTM-081) in an open static system revealed no aquatic toxicity.
The EC50 (0-72h) for Growth rate and Yield were >1.7e-12 mg/L (nominal >100 mg/L) and, thus, could not be determined in this study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)