Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 813-782-0 | CAS number: 5912-87-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-03-10 to 1999-04-12
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Remarks:
- Test substance ID is incomplete as cis/trans isomers ratio is unspecified.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Remarks:
- Statement of compliance with GLP in date of 1999-04-27
- Specific details on test material used for the study:
- No additional information
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aire) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day,
Concentration of sludge: 3.386 g/L (dry weight of suspended solids); to obtain a concentration of 30 mg/L (dry weight) in a 250 mL flask, 2.22 mL of sludge is needed (inoculum). - Duration of test (contact time):
- 32 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium; compliant with recommendations of TG on mineral medium; the pH is measured and if necessary adjusted to 7.4 ± 0.2 with phosphoric acid or potassium hydroxide.
- Solubilising agent (type and concentration if used): None
- Test temperature: 22 °C
- CEC (meq/100 g): No data
- Aeration of dilution water: No data
- Suspended solids concentration: 30 mg/L
- Continuous darkness: No data
- Water: The water used during this study is deionised water containing less than 10 mg/L dissolved organic carbon.
TEST SYSTEM
- Culturing apparatus: Test flasks of the SAPROMAT
- Number of culture flasks/concentration: 2
- Measuring equipment: The respirometer used during this study is a SAPROMAT D 12, made by J. M. VOITH GmbH, D-7920 Heidenheim.
- Test performed in closed vessels: Yes
- Test performed in open system: No
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, inoculum only
- Procedure control: Yes, with the reference substance
- Toxicity control: Yes, test item and reference substance
OTHER:
Toxicity of the test chemical
As suggested in the OECD 301 F method, the toxicity of the test chemical for the inoculum is checked Therefore, a pair of flasks of the volumetric respirometer (SAPROMAT) are filled with mineral medium + test chemical (100 mg/L) + reference substance (100 mg/L) + inoculum and their respirations are recorded as for the other flasks. If they are lower than those of the flasks containing mineral medium + reference substance (100 mg/L) + inoculum, the test chemical can be assumed to be inhibitory to the inoculum used. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg/L
- Preliminary study:
- None
- Test performance:
- Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked. At the end of the test period (normally 28 days), the pH of each flask is measured again
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 28 d
- Remarks on result:
- other: >60% within the 10d window
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 32 d
- Details on results:
- Test substance undergoes 88% biodegradation after 32 days (also 88% after 28 days) in the test conditions. Biodegradation starts on day 2 and reaches 82% at the end of the 10-day window (days 2 to 12).
The curves obtained with the reference substance alone and with test substance + reference substance show no toxic effect of test substance to the micro-organisms at the test concentration. Test substance at 100 mg/L is not inhibitory to the micro-organisms. - Results with reference substance:
- Degradation of sodium benzoate exceeds 40 % after 7 days and 65 % after 14 days: the activity of the inoculum is thus verified and the test is considered as valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Test substance undergoes 88% biodegradation after 28 and 32 days in the test conditions. Biodegradation starts on day 2 and reaches 82% at the end of the 10-day window (days 2 to 12). Thus, test substance should be regarded as readily biodegradable according to this test. In addition, no toxic effects of the test substance were observed to microorganisms at the test concentration.
- Executive summary:
The ready biodegradability of the test item has been determined by the Manometric Respirometry Test according to the OECD Guideline No. 301F with GLP compliance.
A nominal concentration of test substance (100 mg/L) introduce in the system.
Test substance undergoes 88% biodegradation after 28 and 32 days in the test conditions. Biodegradation starts on day 2 and reaches 82% at the end of the 10-day window (days 2 to 12). Test substance at 100 mg/L is not inhibitory to the micro-organisms. Degradation of sodium benzoate exceeds 40 % after 7 days and 65 % after 14 days: the activity of the inoculum is thus verified and the test is considered as valid.
According to this test, the conclusion is that the test item should be regarded as readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
[further information is included as attachment to Iuclid section 13]
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the structural similarity between the source and the target substances. Their environmental properties are expected to be similar because of this structural similarity and because they are anticipated to follow the same environmental fate.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
The target and the source substances are structurally related, in that each has a 2-methyl ether benzene ring, and a propen-2-yl group in 4- position. The source substance is the mixture of the target (i.e. trans-isomer) and cis-isomer. No impurities were reported above 1% of lower if contributing to the hazard of PBT profile. Therefore, it's expected that the impurities will not affect the read-across.
3. ANALOGUE APPROACH JUSTIFICATION
The source substance is not unequivocally identified in the available study (cis and/or trans isomers, cause for restrictions). However, based on structural similarity, no difference on the biodegradability potential is anticipated between trans and cis isomers. Hence, it is considered suitable and scientifically justified to read-across the data between the two substances to fill the biodegradation endpoint in the present dossier.
4. DATA MATRIX
See attached document in Iuclid section 13 - Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across: supporting information
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 28 d
- Remarks on result:
- other: >60% within 10d window
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 32 d
- Details on results:
- Biodegradation starts on day 2 and reaches 82% at the end of the 10-day window (days 2 to 12).
The curves obtained with the reference substance alone and with test substance + reference substance show no toxic effect of test substance to the micro-organisms at the test concentration. Test substance at 100 mg/L is not inhibitory to the micro-organisms. - Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Based on data available on the source substance (88% biodegradation after 28d within the 10d window), the target and the source substances are readily biodegradable and non-toxic to microorganisms up to 100 mg/L.
- Executive summary:
The ready biodegradability of the target substance has been determined by the Manometric Respirometry Test, performed on the source substance according to the OECD Guideline No. 301F with GLP compliance.
A nominal concentration of test substance (100 mg/L) was introduce in the system. Test substance undergoes 88% biodegradation after 28 and 32 days in the test conditions. Biodegradation starts on day 2 and reaches 82% at the end of the 10-day window (days 2 to 12). Test substance at 100 mg/L is not inhibitory to the micro-organisms. Degradation of sodium benzoate exceeds 40 % after 7 days and 65 % after 14 days: the activity of the inoculum is thus verified and the test is considered as valid. According to this test, the conclusion is that the source and the target substance should be regarded as readily biodegradable.
Referenceopen allclose all
Table 5.2.1/2: Test substance - Biological Oxygen Demand (BOD, mg O2/L, adjusted to nominal concentrations)
|
Days: |
2 |
5 |
12 |
21 |
28 |
32 |
|
BOD sludge |
1st flask |
B1 |
4.0 |
10.0 |
14.0 |
18.0 |
22.0 |
22.0 |
2nd flask |
B2 |
0.0 |
6.0 |
8.0 |
10.0 |
14.0 |
14.0 |
|
mean |
B |
2.0 |
8.0 |
11.0 |
14.0 |
18.0 |
18.0 |
|
BOD test substance |
1st flask |
C1 |
34.8 |
152.8 |
189.6 |
201.3 |
208.2 |
209.2 |
2nd flask |
C2 |
31.3 |
149.6 |
187.8 |
201.5 |
209.4 |
209.4 |
|
1st flask corr. |
C1-B |
32.8 |
144.8 |
178.6 |
187.3 |
190.2 |
191.2 |
|
2nd flask corr. |
C2-B |
29.3 |
141.6 |
176.8 |
187.5 |
191.4 |
191.4 |
|
% biodegradation
|
1st flask |
D1 |
15 |
67 |
82 |
86 |
88 |
88 |
2nd flask |
D2 |
14 |
65 |
81 |
86 |
88 |
88 |
|
mean |
D |
14 |
66 |
82 |
86 |
88 |
88 |
Calculations:
B = (B1 + B2) / 2
D1 = 100 * (C1 - B) / ThOD * [S]
D2 = 100 * (C2 - B) / ThOD * [S]
D = (DI + D2)/2
[S] : Initial test substance concentration (mg/L)
ThOD: 2.17 mg O2/mg
Description of key information
Read-across, OECD Guideline 301F, GLP, key study, validity 2:
88% biodegradation after 28 days within the 10-day window. Readily biodegradable. Non-toxic to microorganisms up to 100 mg/L.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
One valid key study is available (Givaudan, 1999), to assess the readily biodegradation of the registered substance, (E)-2 -Methoxy-4 -prop-1 -en-1 -ylphenyl acetate.
This study was performed on a source substance (ratio of cis/trans isomers), according to OECD Guideline 301F and GLP. Based on chemical similarity, no significant difference on the biodegradability potential is anticipated with the present dossier substance (trans isomer), so the read-across is considered justified for a key study. The test substance at 100 mg/L was inoculated with activated sludge from a biological wastewater treatment plant treating predomenantly domestic sewage. The degradation of the test substance was assessed by the determination of the oxygen consumption. The test substance undergoes 88% biodegradation after 28 and 32 days in the test conditions. Biodegradation starts on day 2 and reaches 82% at the end of the 10 day window (days 2 to 12). Therefore, the test substance is considered readily biodegradable. In the toxicity control, containing both the test substance and the reference substance (sodium benzoate), the test substance had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 100 mg/L. In the procedure controls, the degradation of the reference substance exceeds 40% after 7 days and 65% after 14 days, thus confirming suitability of the activated sludge.
In conclusion, according to this study, the registered substance can be considered readily biodegradable and non-toxic to microorganisms up to 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.