Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-282-2 | CAS number: 93-84-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-08-01 to 1990-08-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to current OECD-Guidelines and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Adopted: 04 April 1984
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,3-dihydro-5-nitro-2H-benzimidazol-2-one
- EC Number:
- 202-282-2
- EC Name:
- 1,3-dihydro-5-nitro-2H-benzimidazol-2-one
- Cas Number:
- 93-84-5
- Molecular formula:
- C7H5N3O3
- IUPAC Name:
- 5-nitro-1,3-dihydro-2H-benzimidazol-2-one
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): substance was diluted using water, then homegenised using ultraturrax and ultrasonic bath; the so prepared test substance was transferred to the testing vessel with stirring. There the whole liquid was stirred for another 2hrs using a glass rod and kpg-stirrer
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): target concentration: 500mg/L, actual measured: 92,9mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): precipitate on the ground
- Concentration was determined at the beginning of the experiment and after 48hrs and 96hrs using HPLC/UV-vis
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 22+/-1°C
- pH:
- pH = 8,3
- Dissolved oxygen:
- >=80%
- Details on test conditions:
- see below
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 92.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 92.9
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: impaired swimming behaviour
- Other biological observations: no lethality neither in the 92,9mg/L (actual concentration, target was 500mg/L) Nitrolon TF nor in the control group
- Abnormal responses: depending on time and to different extent: reduced activity, swimming near to the water surface, irregular breathing...more details see attached table "Symptomatik"
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: precipitation of test material on the ground
Any other information on results incl. tables
- Sublethal observations / clinical signs:
To each vessel (control, test substance) 10 fishes were randomly distributed to. Fishes were not feeded during the experiment.
At hours 2 - 4 - 24 - 72 and 96 the tanks were rated for adverse reactions. Though no lethality over the test period was observed, the fishes showed altered behaviour, symtomps increased in number and abundance, correlated with increasing duration of the test period.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- with restrictions due to solubility maximum
- Conclusions:
- No lethality at max solubility of 92,9 mg/l after 48 and 96 hours. Therefore LC50 is above 92,9 mg/l.
- Executive summary:
The test of 5 -Nitrobenzimidazolon TF on Danio rerio over 96 hours revealed no lethal effect at a centration of 92,9 mg/ l (mean), together with unsolved substance residue 500 mg/l test substance in the vessel was present. The test solution was a yellow liquid with excess of test substance as sediment.
At test begin, after 48 and 96 hours a probe from the middle of the water column and in the tank middle was analysed for the concentration of the test substance in the solution. Arithmetic mean was below 80% of the reference concentration of 500 mg/l. Due to concistency of the measured value over the test period, the LC50 is given for the present concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.