Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 265-529-3 | CAS number: 65151-26-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Remarks:
- According to OECD 301B but limited documentation regarding the test material
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- no
Test material
- Reference substance name:
- Disodium 7-[(4-amino-3-methoxyphenyl)azo]naphthalene-1,3-disulphonate
- EC Number:
- 265-529-3
- EC Name:
- Disodium 7-[(4-amino-3-methoxyphenyl)azo]naphthalene-1,3-disulphonate
- Cas Number:
- 65151-26-0
- Molecular formula:
- C17H15N3O7S2.2Na
- IUPAC Name:
- disodium 7-[(4-amino-3-methoxyphenyl)diazenyl]naphthalene-1,3-disulfonate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal biological sewage treatment plant, not adapted, not pre-conditioned; ARA Werdhoelzli, 8048 Zuerich, Switzerland
- Concentration of sludge: 0.2 g/L dry matter in the final mixture
- Preparation of inoculum for exposure: prior to the test the sludge was washed twice with tap water - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 156 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Test sample: the test material was diluted with mineral salts medium to give a final DOC concentration of about 50 mg/L
TEST CONDITIONS
- Composition of medium: as specified in OECD 302B
- Test temperature: 22 ± 2°C
- Aeration of dilution water: yes, with CO2-free air
- Concentration reference substance: 49.6 mg/L DOC/L
TEST SYSTEM
- Culturing apparatus: 1200 mL closed glass bottles containing a total volume of test solution of 600 mL
- Number of culture flasks/concentration: 2 replicates per test item concentration; 2 replicates per blank control, 1 replicate per positive control
- Measuring equipment: DOC was determined with Shimadzu 5050 TOC-Analyzer using the NPOC-mode; each analysis was carried out in duplicate
- Details of trap for CO2: 120 mL of 0.5 m sodium hydroxide solution respectively
- Sampling: samples were taken on days 1, 4, 7, 11,14, 18, 21, 25, 27 and 28
Reference substance
- Reference substance:
- diethylene glycol
Results and discussion
- Preliminary study:
- not applicable
- Test performance:
- not applicable
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Based on the data of the individual DOC determinations no biodegradation of the test item was observed after 28 days of incubation.
BOD5 / COD results
- Results with reference substance:
- The positive control, diethyleneglycol, showed 99 % biodegradation after 14 days of incubation thus confirming suitability of inoculum and test conditions.
The test was considered valid, since the degradation of the reference compound reached more than 70 % within 14 days of incubation.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- No biodegradation of the test item was observed after 28 days.
- Executive summary:
In the Zahn-Wellens test on the test substance according to OECD 302B, no biodegradation was observed after 28 days. The substance has to be regarded as not inherently biodegradable.
Diethyleneglycol attained 99 % degradation after 14 days thereby confirming the suitability of the inoculum and test conditions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.