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EC number: 228-711-3 | CAS number: 6334-18-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,3-dichlorobenzaldehyde
- EC Number:
- 228-711-3
- EC Name:
- 2,3-dichlorobenzaldehyde
- Cas Number:
- 6334-18-5
- Molecular formula:
- C7H4Cl2O
- IUPAC Name:
- 2,3-dichlorobenzaldehyde
- Test material form:
- solid: crystalline
- Details on test material:
- Test item: 2,3-dichlorobenzaldehydeCas No.: 6334-18-5Storage: 15-30 °C
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Species and strain: New Zealand white rabbitSource: S&K LAP Kft.Hygienic level during the study: good conventionalNumber of animal: 3 malesAge of animals: adult rabbits, 13 weeks oldBody weight range at the beginning of the study: 2200 - 2520 gBody weight range at the end of the study: 2250 - 2580 gAcclimatisation time: 10 days in first animal and 11 days in second and third animalHusbandryAnimal health: Only animals in acceptable health condition were used for the test.Housing: Animals were housed individually in metal cage.Light: 12 hours daily, from 6.00 a.m. to 6.00 p.m.Temperature: 20 ± 3 °CRelative humidity: 30-70 %Ventilation: 10-15 air exchanges/hour by central air-condition system
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- 0.1 g of the finely grounded test item was used for the study, as a single dose.
- Duration of treatment / exposure:
- The eyes of the test animals were not washed out 24 hour after test item application, because the test item had been removed from the eye of test animal by physiological mechanisms.
- Observation period (in vivo):
- The eyes were examined at 1, 24, 48 and 72 hours, then 1week after the application.
- Number of animals or in vitro replicates:
- Three male animals in acceptable health condition were selected for the test.
- Details on study design:
- Procedure:The test item was applied in a single dose to the left eye of animals. The degree of irritation was observed and scored at specified intervals. Information derived from this test served to indicate the existence of possible hazards likely to arise from exposure of the eyes and associated mucous membranes to the test item. At termination animals were sacrificed.A Single Animal Test:In the first step an initial test was performed using one animal. The test item was placed into the conjunctival sac of the left eye. The eyelids were held closed gently for about one second to prevent any loss of the test item. The contralateral eye served as control.In the initial test no irritant effect was observed 24 hours after the treatment. The confirmatory test was conducted using a second and third animal.Application of the Test Item:The test item was instilled into the conjunctival sac of the left eye of each animal. The eye lids were gently held closed for about one second to prevent the loss of the test item. The contralateral eye serves as control.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1.66
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Irritation parameter:
- iris score
- Basis:
- animal: 1; 2; 3
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: 2; 3
- Time point:
- 24/48/72 h
- Score:
- 1
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Irritation parameter:
- other: conjunctival discharge
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- other: conjunctival discharge
- Basis:
- animal: 2; 3
- Time point:
- 24/48/72 h
- Score:
- 0.66
- Other effects:
- No systemic toxicity was observed on the day of the treatment and during the 1-week observation period. The body weight of animals corresponded to their species and age. Sign of pain and distress as discharge was observed in all animals. This symptom was detected between the treatment day and first observation of Day 1.
Applicant's summary and conclusion
- Interpretation of results:
- Category 2B (mildly irritating to eyes) based on GHS criteria
- Conclusions:
- In conclusion, test item 2,3-DICHLORO-BENZALDEHYDE (CAS 6334-18-5) applied to the rabbits' eye mucosa caused slight to severe conjunctival and slight to moderate cornea irritant effects which were fully reversible within 1 week.The test item can be classified into hazard Category 2B for eye irritation.
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