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EC number: 201-184-7 | CAS number: 79-19-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Link to relevant study records
- Endpoint:
- in vitro DNA damage and/or repair study
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- The genotoxicity of Thiosemicarbazide was studied in Hepatocyte primary culture/DNA repair test using rat hepatocyte
- GLP compliance:
- no
- Type of assay:
- DNA damage and repair assay, unscheduled DNA synthesis in mammalian cells in vitro
- Species / strain / cell type:
- hepatocytes: ACI/N rats
- Details on mammalian cell type (if applicable):
- - Type and identity of media: The hepatocytes isolated from the liver of ACI/N rats were allowed to attach for 2 hr to plastic coverslips in primary culture using Williams’ Medium E for further experiment to be performed.
- Properly maintained: No data available
- Periodically checked for Mycoplasma contamination: no data
- Periodically checked for karyotype stability: no data
- Periodically "cleansed" against high spontaneous background: no data - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- not specified
- Test concentrations with justification for top dose:
- 10-³,10-⁴,10-⁵M (0.001, 0.0001, 0.00001 M)
- Vehicle / solvent:
- Distilled water
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: N-2-fluorenylacetamide
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Preincubation period: No data
- Exposure duration: 20 hrs
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): 14 days
- Fixation time (start of exposure up to fixation or harvest of cells): No data
SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data
NUMBER OF REPLICATIONS: 2
NUMBER OF CELLS EVALUATED: 50 cells/3 coverslips
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data
OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data
OTHER: - Evaluation criteria:
- The test compound judged positive when the mean nuclear grain count was more than 5 grains or positive cells were more than 33%.
- Statistics:
- No data available
- Species / strain:
- hepatocytes: ACI/N rats
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
- Conclusions:
- Interpretation of results (migrated information):
negative
In the DNA repair test with rat hepatocytes, thiosemicarbazide showed negative response. Therefore, the genotoxicity of thiosemicarbazide is negative in rat hepatocytes. - Executive summary:
The Genotoxicity of thiosemicarbazide was examined in Hepatocyte primary culture/DNA repair test on rat hepatocytes.
The test was performed basically in accordance with the methods of Williams et al. Hepatocytes were isolated from the livers of ACI/N rats weighing 200-250g.The isolated hepatocytes were allowed to attach for two hr to plastic coverslips in primary culture using Williams’ Medium E.The culture were washed and exposed to test compound at 10¯³, 10¯⁴, 10¯⁵M (0.001, 0.0001, 0.00001 M) doses.N-2-fluorenylacetamide used as a positive control substance.
In the DNA repair test, all the numbers of UDS grains/nucleus of test compound or control were negative value. It means thiosemicarbazide have negative DNA repair response.
Therefore, the genotoxicity of thiosemicarbazide is negative in rat hepatocytes.
Reference
The results of DNA repair test:
Chemical |
Dose(M) |
UDS grains/nucleus |
DNA repair |
Thiosemicarbazide |
0.001 |
-1.0 ± 1.5 |
- |
0.0001 |
-1.2 ± 1.4 |
- |
|
0.00001 |
-0.8 ± 1.3 |
- |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Studies were reviewed for genetic toxicity from reliable sources having Klimisch rating 2
The summary of the results are presented below:
Sr.No. |
Endpoint |
Species/ Strain |
Result |
Sources |
1. |
Genetic toxicity |
Rat hepatocytes |
Negative |
Experimental data for target chemical |
2. |
Genetic toxicity |
Mouse hepatocytes |
Negative |
Experimental data for target chemical |
Applying weight of evidence approach to the above studies, the substance thiosemicarbazide is not expected to be an in vitro mutagen.
Justification for selection of genetic toxicity endpoint
In the DNA repair test with rat hepatocytes, thiosemicarbazide showed negative response. Therefore, the genotoxicity of thiosemicarbazide is negative in rat hepatocytes.
Justification for classification or non-classification
On the basis of available information, the substanceThiosemicarbazide is not expected to be mutagenic in nature.
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